Gene Information

Name : PFLU2558 (PFLU2558)
Accession : YP_002872146.1
Strain : Pseudomonas fluorescens SBW25
Genome accession: NC_012660
Putative virulence/resistance : Resistance
Product : methionine gamma-lyase
Function : -
COG functional category : E : Amino acid transport and metabolism
COG ID : COG0626
EC number : 4.4.1.11
Position : 2823017 - 2824252 bp
Length : 1236 bp
Strand : -
Note : catalyzes the formation of methanethiol and 2-ocobutanoate from L-methionine

DNA sequence :
ATGAACAATAAACACAACACATTCGGCTTTTCCACCCGCGCCATCCACCACGGCTACGACCCCAAGGACCATCACGGCGC
GCTCGTACCGCCGATTTACCTGTCTGCCACCTTCGCATTCCCCACCGCCGAATACGGCGCCGCGTGCTTTGCCGGCGAAG
CCGCCGGCCACTTTTATACGCGCATTTCCAACCCGACCCTGGCACTGCTGGAGTCACGCATGGCGACGTTGGAAAACGGC
GAGGCGGCAGTGGCTTTCAGTTCCGGCATGGGGGCAATCGCCGCCACGTTCTGGACCCTGCTGCGTCCCGGTGATGAAGT
AATCGTCAGTCAGACGCTGTACGGCTGCACCTTCGCGCTACTGCACCACGGCATCGGCGAGTTCGGTATCAAGGTGCGGC
ATGTCGACCTCACCGACCTGGCCGCCCTGCAAGCGGCACTCTGCCCCGCCACCCGTATGATCTACTGCGAAACCCCGGCC
AACCCCACCTTGCAACTGGTGGATATCGCCGCCGTGGCCGCCCTCGCCCATCAGCAGCCCAACGTCACCGTGGTGATCGA
CAACACCTACTGCACCCCCTATCTGCAACGCCCCCTCGACCTGGGCGCCGACGTGGTGGTGCATTCGGCCACCAAGTACC
TCAGCGGCCACGGCGATATCACCGCGGGCATCGCCGTGAGCAGCCAGGCCCTGGCCCAGCGTATTCGCCTGCAAGGCCTC
AAGGACCTGACCGGCGCGGTGATGTCGCCCCAGGACGCCTCGCTATTGATGCGCGGCCTCAAGACCCTGGCCCTGCGCAT
GGACCGCCATTGCAGCAATGCCCAGGCGGTGGCCGAAGCCTTGCAGGCGCACCCGGCCGTGCAGTGGGTGACTTACCCCG
GTCTGCGCTCTTTCGCCCAATACGAACTGGCGACACGGCAGATGAAACTGCCTGGCGGCATGATTGCCTTCGAACTCAAG
GGCGGGATCGACACCGGCCGGCGCTTCATGAATGCGCTCACAGTGTTCACGCGAGCGGTCAGCCTAGGGGATGCAGAGTC
ACTGGCCCAGCACCCGGCGAGCATGACCCATTCCACCTACACACCGGAGGAGCGTGCGGCGCACGGCATCAGCGAAGGGT
TGGTGCGTTTGTCGGTGGGGCTGGAAGATATCGCCGACCTGCTGGCGGATGTGCAACAGGCGCTGGCCAAGTGCGCCCGT
ACTGTTACGCGTCCTTCTAAGCTTGTGAATATTTAA

Protein sequence :
MNNKHNTFGFSTRAIHHGYDPKDHHGALVPPIYLSATFAFPTAEYGAACFAGEAAGHFYTRISNPTLALLESRMATLENG
EAAVAFSSGMGAIAATFWTLLRPGDEVIVSQTLYGCTFALLHHGIGEFGIKVRHVDLTDLAALQAALCPATRMIYCETPA
NPTLQLVDIAAVAALAHQQPNVTVVIDNTYCTPYLQRPLDLGADVVVHSATKYLSGHGDITAGIAVSSQALAQRIRLQGL
KDLTGAVMSPQDASLLMRGLKTLALRMDRHCSNAQAVAEALQAHPAVQWVTYPGLRSFAQYELATRQMKLPGGMIAFELK
GGIDTGRRFMNALTVFTRAVSLGDAESLAQHPASMTHSTYTPEERAAHGISEGLVRLSVGLEDIADLLADVQQALAKCAR
TVTRPSKLVNI

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SAR0460 YP_039909.1 Cys/Met metabolism PLP-dependent enzyme Not tested vSa¥á Protein 4e-54 42
SAS0418 YP_042545.1 Cys/Met metabolism PLP-dependent enzyme Not tested vSa¥á Protein 3e-55 42

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
PFLU2558 YP_002872146.1 methionine gamma-lyase BAC0207 Protein 5e-140 75