Gene Information

Name : topB (PFLU2157)
Accession : YP_002871773.1
Strain : Pseudomonas fluorescens SBW25
Genome accession: NC_012660
Putative virulence/resistance : Unknown
Product : DNA topoisomerase III
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0550
EC number : 5.99.1.2
Position : 2335944 - 2337893 bp
Length : 1950 bp
Strand : -
Note : decatenates replicating daughter chromosomes

DNA sequence :
ATGCGGCTGTACCTCTGTGAAAAACCCTCCCAGGCCAAGGATATTGCGGCCGTGCTCGGCGCCAGCCGCCGGGGCGACGG
TTGCTGGCTGGGCAATGGGATCACAGTCACCTGGTGCATCGGCCACCTGTTGGAAACCGCCCCGCCCGATGCGTACGATG
CCAAGTACAAACGCTGGGTGCTGGCCGACCTGCCCATCGTCCCGGAAAAATGGAAGATGCTGGTCAAACCCAAGACCGCC
AGCCAATTCAAGGTGGTCAAGCGACTGCTGGGCGAAGCGCAGGAATTGGTGATCGCCACCGACGCCGACCGTGAAGGCGA
GATGATCGCGCGCGAGTTGGTGGAGCATTGCCGCTATCGCGGGCCGATCCAGCGGCTGTGGCTGTCGGCGCTCGACGATG
CCTCGATTCGCAAGGCCCTGGCGGCCCTCAAACCCGGCGCCGAGACCTTCAGCCTGTATCACTCGGCCCTGGGCCGCTCC
CGCGCCGACTGGTTGATCGGCATGAACATGAGCCGCCTGTTCACGCTGCTCGGGCGCCAGTCCGGTTACCAGGGCGTATT
GCCCGTAGGTCGGGTGCAAACGCCGACCCTGCGCCTGGTGGTGGACCGCGACCGCAGCATTGCGGATTTCGTGCCGGTGG
CTTACTGGGCCATCGATGTCGACCTGCACCACGAGCGCATGACCTTCACCGCCCAATGGCGCGCGGCCGAGGAGGCCTGT
GATGACCAGGGCCGCTGCCTCAACCCGCAACTGGCGCGTGACGCCGCCGATGCCATGGCCAATGCCGCCACGGCGCGACT
GGTGAAGCTACGCACCGAACGCATGCGTGAGGTCGCGCCCCTGCCCTTCGACCTCGGCACCCTGCAGGAAATCTGCTCCA
AGAAACTCGGCCTCGGCGCTCAGGAAACCCTGGATATTGCCCAGTCGCTGTATGAAACCCACAAAGTCATCACCTACCCC
CGCAGCGATTGCGGCTACCTGCCGGTCAGTCAGCACAGCGAAGCGCCGAAAATTCTGGCTGCGCTGGGACGCGCTGATGC
AGCGGTAAACGCATTGATGCCGCATATCGATCCCCAGCGTCGCTCGCGGGCGTGGAACGACGCCAAGGTCAGCGCCCACC
ACGGCATCATCCCGACCGGTGCCGGCAAGGAGGTTGGGCAACTGACCGGTAAGCATCGTGCGGTGTACACATTGATTCGC
GCGCGCTACCTGGCGCAGTTCCTGCCCAACCATGAATACGATCGCACCCAAGCGGATTTCGACTGTGCGGGCCAAGCATT
GCGTGCGGTGGGCAAAGTGGTGATCGAGCCGGGTTGGAAACGCGCCCTGCCCGAAGCACTCACACCGGCTAAGGGCCGTG
AAGCGCCTGTGCCGCAGGCATTGCCGACATTGGTGCAGGGCCAGGATTACGCGGTGGCCAAGGTCAACCTCAAGGACCTC
TGGACCCAACCACCCAAGCCCTTCACCGAAGGCGACCTGATCAAGGCGATGAAAAACGTCGCCAAGCTGGTGGAAGACCC
GCTGCTCAAGCAAAAGCTCAAGGACACCACCGGCATCGGCACCGAAGCCACCCGCGCCGGGATTATCCAAGGGTTGCTCG
ACCGAGGTTACCTGGTGAAGAACGGCAAGGCGCTGTCGGCGACACCGGCGGCGTTCAGCCTGATCGACGCGGTGCCCCGC
GCCATCGCCGACCCTGGCACTACCGCGATCTGGGAGCAGGCGCTGGACATGGTACAAAGCGGCGAAATGAGCCTGGAAGA
ATTCGTCGCCAAACAGGCGGCCTGGATGAGCAAGCAGGTGGCGCGCTGCAATGGCATGCGCATGACCATCACCGGCCCGG
CCAGCCCGGCAGGTCGAGGCGCTACGCCGTGGAAAAACAAACGCAAGAGCGCGCCACGCAAGACGACAGCCAGCCCCAAG
CGGGCAAAAAAGCCGACAAATGCTGGGTGA

Protein sequence :
MRLYLCEKPSQAKDIAAVLGASRRGDGCWLGNGITVTWCIGHLLETAPPDAYDAKYKRWVLADLPIVPEKWKMLVKPKTA
SQFKVVKRLLGEAQELVIATDADREGEMIARELVEHCRYRGPIQRLWLSALDDASIRKALAALKPGAETFSLYHSALGRS
RADWLIGMNMSRLFTLLGRQSGYQGVLPVGRVQTPTLRLVVDRDRSIADFVPVAYWAIDVDLHHERMTFTAQWRAAEEAC
DDQGRCLNPQLARDAADAMANAATARLVKLRTERMREVAPLPFDLGTLQEICSKKLGLGAQETLDIAQSLYETHKVITYP
RSDCGYLPVSQHSEAPKILAALGRADAAVNALMPHIDPQRRSRAWNDAKVSAHHGIIPTGAGKEVGQLTGKHRAVYTLIR
ARYLAQFLPNHEYDRTQADFDCAGQALRAVGKVVIEPGWKRALPEALTPAKGREAPVPQALPTLVQGQDYAVAKVNLKDL
WTQPPKPFTEGDLIKAMKNVAKLVEDPLLKQKLKDTTGIGTEATRAGIIQGLLDRGYLVKNGKALSATPAAFSLIDAVPR
AIADPGTTAIWEQALDMVQSGEMSLEEFVAKQAAWMSKQVARCNGMRMTITGPASPAGRGATPWKNKRKSAPRKTTASPK
RAKKPTNAG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
topB AAN62194.1 putative DNA topoisomerase III Not tested PAGI-2(C) Protein 0.0 62
pph56 CAI36097.1 Topoisomerase IA Not tested PPHGI-1 Protein 0.0 62
topB AAN62317.1 putative DNA topoisomerase III Not tested PAGI-3(SG) Protein 0.0 61
topB NP_458624.1 topoisomerase B Not tested SPI-7 Protein 7e-150 49
topB NP_807836.1 DNA topoisomerase III Not tested SPI-7 Protein 7e-150 49
topB AAF14809.1 topoisomerase B Not tested SPI-7 Protein 5e-150 49
PMI2630 YP_002152332.1 DNA topoisomerase III Not tested Not named Protein 7e-149 49
topB1 YP_005176228.1 DNA topoisomerase III Not tested ICEPmu1 Protein 7e-145 48
topB AAW83109.1 TopB Not tested GGI Protein 1e-113 43