Gene Information

Name : topB (PFLU1972)
Accession : YP_002871597.1
Strain : Pseudomonas fluorescens SBW25
Genome accession: NC_012660
Putative virulence/resistance : Unknown
Product : DNA topoisomerase III
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0550
EC number : 5.99.1.2
Position : 2143376 - 2145343 bp
Length : 1968 bp
Strand : -
Note : decatenates replicating daughter chromosomes

DNA sequence :
ATGCGCCTCTTCCTCTGCGAAAAACCTTCCCAGGGTCGGGACATCGCCACGGTACTCGGGGCCACTCGGCGTGGTGATGG
TTGCCTGATCGGCACAGAGACAACCGTCACCTGGTGTATCGGCCACCTACTGGAGACAGCACCGCCTGAAGCCTATGGCA
TTCAGTACAAGAACTGGTCGTTGGATCATCTACCGATCATTCCCGCGCAGTGGAAGGTCGAGGTCAAACACAAAACTGCG
GCACAGTTCAAAGTCATCAAGCGCCTGCTCAGTGAAGCCTCTGCTGTCGTCATCGCGACCGACGCCGATCGCGAAGGTGA
GATGATTGCCCGCGAGTTACTGGAACTTTGCAACTATCGAGGTCCCGTTGAGCGCCTCTGGTTGTCCGCACTCAACGAGG
TGTCGATTCTCAAAGCGCTGTCCTCGCTGAAGTCTGGTCAGGAGACCTTCCCGCTCTACCACTCAGCCCTCGCCCGCAGC
CGGGCCGACTGGCTGATCGGCATGAACCTCAGTCGTTTGTTTACCCTGCTCGGTCGACGAGCGGGCTACGACGGTGTGCT
GTCAGTGGGACGCGTCCAGACACCCACCTTACGTCTGGTGGTCGATCGGGATCGTGCGATTGCCAGCTTCGTCTCGGTGC
CCTACTGGGACGTGGAGGTGCACCTGTCCTTCATGGGGCAATCATTCATCGCGTCGTGGTTACCACCCAGTTCAGGACGA
GATGAGGCCTGTCGTTGCCTGCAGCAGGCGCTTGCCCGTCATGCTGGCCAAACAATCACTGACGGTAAGACCGCCACAGT
ACTCTCGTTGCAGACTGAGCATTTCCGAGAACCGCCGCCTCTGCCCTTCGACCTGAGCACACTGCAAGAAGTCTGCTCGC
GCAAATTGGGGCTAGGGGTCCAGGAAACCCTGAACATCGCCCAAGCCCTGTATGAAACCCACAAAGCCACCACCTACCCG
CGCAGCGATTGCCGCTATTTGCCAGCGAGTATGTTCGACGAGGTACCCACGGTATTCGATGCCCTGCTCAAAACGGATTC
CGCACTACAGCCCGCATTTGAAAAACTTGATCGGTCATTACGTTCCCGCGCCTGGAACGATGCCAAAGCCACCGCGCACC
ACGGCATCATCCCCACCACCGAGCCGGCAAACCTGGCGCGGATGTCCGAGCAAGAACGCCAAGTCTACGAACAGATTCGT
AACCACTACCTCGCGCAATTTCTGCCGCACCATGAATTTGATCGAACCCAGGTCGAACTGGAGTGTGGCAAAGAACGATT
GACCGCGGTTGGCAAACAGATCCTGGTCCAAAGCTGGAAAGGCTTGCTCTACGACAACACCGAGGACGATGAGCCCAGTC
AAAAATTCCAAGTCTTACCCGTCTTGCAACAGGGTACGCAGTGCGCAGTAAACGACGTCGAACTCAAGTCTATGCGCACC
ACAGCTCCCAAACCGCTGACCGAGGGCGATCTGATCAAGGCGATGAAAAACGTGGCCAAGCTGGTCAACGACCCGCGTCT
GAAACAGAAGCTTCGGGACACCACCGGCATCGGCACCGAAGCCACGCGTGCCGGCATCATCAAGGGCTTGATCGATCGCG
GTTATTTGCTGAAGAAAAAACGCGCCTTAATGGCCTCCGCCGCGGCCCATACCCTGATTGAGGCGGTACCCGCGGCCATC
GCAGATCCGGGCATGACCGCGATTTGGGAGCAGGCTCTGGACGAAATCGAAGCGGGACGTCTGAGCCTGGATGCATTCGT
CGCCAAGCAGGCGAGTTGGATTGCGCAATTGGTCGAACACTGCCGAGCGCTCACCTTGGCGTTACCGGTCGAAACCGGAC
CGGCTTGCCCCATTTGCAACGCCTCTATGCTCAGACGTACGGGAAAATCAGGGGCGTTCTGGTCGTGCTCCCACTACCCC
GACTGCAAGGGCACTGTGCCGATCAGCAAAGATATACGGCGCCCATGA

Protein sequence :
MRLFLCEKPSQGRDIATVLGATRRGDGCLIGTETTVTWCIGHLLETAPPEAYGIQYKNWSLDHLPIIPAQWKVEVKHKTA
AQFKVIKRLLSEASAVVIATDADREGEMIARELLELCNYRGPVERLWLSALNEVSILKALSSLKSGQETFPLYHSALARS
RADWLIGMNLSRLFTLLGRRAGYDGVLSVGRVQTPTLRLVVDRDRAIASFVSVPYWDVEVHLSFMGQSFIASWLPPSSGR
DEACRCLQQALARHAGQTITDGKTATVLSLQTEHFREPPPLPFDLSTLQEVCSRKLGLGVQETLNIAQALYETHKATTYP
RSDCRYLPASMFDEVPTVFDALLKTDSALQPAFEKLDRSLRSRAWNDAKATAHHGIIPTTEPANLARMSEQERQVYEQIR
NHYLAQFLPHHEFDRTQVELECGKERLTAVGKQILVQSWKGLLYDNTEDDEPSQKFQVLPVLQQGTQCAVNDVELKSMRT
TAPKPLTEGDLIKAMKNVAKLVNDPRLKQKLRDTTGIGTEATRAGIIKGLIDRGYLLKKKRALMASAAAHTLIEAVPAAI
ADPGMTAIWEQALDEIEAGRLSLDAFVAKQASWIAQLVEHCRALTLALPVETGPACPICNASMLRRTGKSGAFWSCSHYP
DCKGTVPISKDIRRP

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
topB AAN62194.1 putative DNA topoisomerase III Not tested PAGI-2(C) Protein 0.0 65
topB AAN62317.1 putative DNA topoisomerase III Not tested PAGI-3(SG) Protein 0.0 65
pph56 CAI36097.1 Topoisomerase IA Not tested PPHGI-1 Protein 0.0 64
topB NP_807836.1 DNA topoisomerase III Not tested SPI-7 Protein 4e-169 51
topB AAF14809.1 topoisomerase B Not tested SPI-7 Protein 3e-169 51
topB NP_458624.1 topoisomerase B Not tested SPI-7 Protein 4e-169 51
PMI2630 YP_002152332.1 DNA topoisomerase III Not tested Not named Protein 1e-161 50
topB1 YP_005176228.1 DNA topoisomerase III Not tested ICEPmu1 Protein 2e-163 50
topB AAW83109.1 TopB Not tested GGI Protein 2e-122 41