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Name : lysS (PFLU1228) Accession : YP_002870881.1 Strain : Pseudomonas fluorescens SBW25 Genome accession: NC_012660 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 1362578 - 1364080 bp Length : 1503 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGAGCGACCAACAACTCGACCCGCAAGCCCTGCAACAGGAAGAAAACTCCCTGATCGCCCTGCGCAAGGAAAAGCTGGC TGCCGAGCGCGCCAAGGGCAATGCCTTCCCCAACGACTTCCGCCGCGAAAACTACTGCGATGCCTTGCAGAAGCAATACG CGGACAAGACCAAGGAAGAGCTGGCAGAGGCTGCGATTCCGGTCAAGGTGGCAGGTCGCATCATGCTTAACCGTGGCTCG TTCATGGTGATCCAGGACATGACCGGGCGTATCCAGGTCTACGTCAACCGCAAGACCCTGTCCGAAGACACCCTGGCCTC GGTGAAAACCTGGGACATGGGCGACATCATCGCCGCCGAAGGCACCCTGGCCCGTTCCGGCAAGGGCGACCTGTACGTGG AAATGACCAGCGTGCGGCTGCTGACCAAATCCCTGCGCCCGCTGCCGGACAAGCACCACGGCCTGACCGACACCGAACAG CGCTATCGCCAGCGCTACGTTGACCTGATCGTCAACGAAGACGTGCGCCAGACCTTCCGCGTGCGTTCGCAAGTCATCGC CCACATCCGCAGCTTCCTGATGCAGCGTGACTTCCTGGAAGTGGAAACCCCGATGCTGCAAACCATCCCCGGTGGTGCCG CAGCCAAGCCGTTCGAAACCCACCACAACGCGCTGGACCTGGAAATGTTCCTGCGTATTGCGCCTGAGCTGTACCTCAAG CGCCTTGTTGTTGGCGGCTTCGAGAAGGTGTTCGAGATCAACCGCAACTTCCGTAACGAAGGCGTCTCGACCCGTCACAA CCCAGAATTCACCATGTTGGAGTTCTACCAGGCCTACGCCGACTACGAAGACAACATGGACCTGACCGAAGAACTGTTCC GCGAGCTGGCACAGCTGGTTCTGGGCAGCACCGACGTGCCGTACGGCGACAAGGTGTTCCACTTCGGCGAGCCGTTCGTG CGTCTGTCGGTATTCGATTCGATCCTCAAATACAACCCTGAGCTGACCGCCGACGACCTGAACGACATCGACAAGGCTCG TGCGATCGCCAAGAAAGCCGGCGCCAAGGTGCTGGGCTTCGAAGGCCTGGGCAAATTGCAGGTGATGATTTTCGAAGAGC TGGTGGAGCACAAGCTGGAGCAGCCGCACTTCATTACTCAGTACCCGTTCGAAGTGTCGCCGCTGGCCCGTCGCAACGAT GACAACCCGAACGTCACCGACCGTTTCGAGCTGTTCATCGGCGGCCGCGAAATCGCCAACGCCTACTCCGAGCTCAACGA CGCGGAAGACCAGGCCGAGCGCTTCATGGCCCAGGTGGCCGATAAGGACGCCGGCGATGACGAAGCCATGCACTACGATG CTGACTTCGTACGCGCCCTGGAATACGGCATGCCGCCCACCGCCGGTGAAGGTATCGGCATCGACCGCCTGGTGATGCTG CTGACCAACTCGCCGTCGATCCGTGACGTGATCTTGTTCCCGCACATGCGGCCGCAAGCGTAA Protein sequence : MSDQQLDPQALQQEENSLIALRKEKLAAERAKGNAFPNDFRRENYCDALQKQYADKTKEELAEAAIPVKVAGRIMLNRGS FMVIQDMTGRIQVYVNRKTLSEDTLASVKTWDMGDIIAAEGTLARSGKGDLYVEMTSVRLLTKSLRPLPDKHHGLTDTEQ RYRQRYVDLIVNEDVRQTFRVRSQVIAHIRSFLMQRDFLEVETPMLQTIPGGAAAKPFETHHNALDLEMFLRIAPELYLK RLVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYEDNMDLTEELFRELAQLVLGSTDVPYGDKVFHFGEPFV RLSVFDSILKYNPELTADDLNDIDKARAIAKKAGAKVLGFEGLGKLQVMIFEELVEHKLEQPHFITQYPFEVSPLARRND DNPNVTDRFELFIGGREIANAYSELNDAEDQAERFMAQVADKDAGDDEAMHYDADFVRALEYGMPPTAGEGIGIDRLVML LTNSPSIRDVILFPHMRPQA |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 8e-134 | 60 |
| lysS | YP_005163386.1 | lysyl-tRNA synthetase | Not tested | Not named | Protein | 3e-77 | 43 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_002870881.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 5e-134 | 60 |
