Gene Information

Name : murD (PFLU0945)
Accession : YP_002870607.1
Strain : Pseudomonas fluorescens SBW25
Genome accession: NC_012660
Putative virulence/resistance : Unknown
Product : UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG0771
EC number : 6.3.2.9
Position : 1048901 - 1050247 bp
Length : 1347 bp
Strand : +
Note : UDP-N-acetylmuramoylalanine--D-glutamate ligase; involved in peptidoglycan biosynthesis; cytoplasmic; catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine during cell wall formation

DNA sequence :
GTGTCCCTGATCGCTTCAGACCACTTCCGCATCGTTGTCGGCCTCGGCAAGAGCGGCATGTCCCTGGTTCGCTTCCTGGC
GAACCGGGGCAAGCCGTTTGCTGTGGCCGATACGCGGGAAAATCCACCGGAGCTGGTCACGCTGCGCCGTGACTACCCGC
ACGTGGAAGTGCGTTGTGGCGAGCTGGATGTCGAGTTTCTGTGCCGTGCCGATGAGCTCTACGTGAGCCCCGGCCTGGCG
TTGGCGACACCAGCCCTGCAAGCCGCAGCGGCGCGTGGCGTGAAACTGTCCGGCGATATCGACCTGTTCGCGCGTCACGC
GAAGGCGCCGATCGTGGCGATCAGTGGTTCCAACGCAAAAAGCACCGTCACCACCCTGGTTGGCGAGATGGCTGTGGCGG
CCGGTAAGCGTGTCGCCGTGGGCGGCAACCTCGGTACGCCGGCGCTGGACCTGCTCAGCGACGACATCGAGTTGTACGTG
ATGGAGCTGTCGAGCTTCCAGCTGGAAACGACTCACGACCTTGGTGCCGAAGTTGCCACCGTCTTGAACGTCAGCGAAGA
TCACATGGACCGCTACAGCGGCCTGCCGGCCTATCACCTGGCCAAGCACCGAATCTTCCGCGGCGCCAAGCAAGTGGTGG
TCAACCGCCAGGATGCGCTGAGCCGTCCGCTGATGGGCGAGGGCCTGCCGTGCTGGACCTTCGGCCTGAGCAAACCCGAT
TTCAAAGCCTTCGGCATCCGCGAAGAGCACGGCGAGAAGTACCTGGCCTTCGAATTCCAGAACCTGATGCCGGTGCGCGA
GCTGAAAATTCGTGGCGCGCATAACCAGTCCAACGCCCTTGCGGCCTTGGCGCTCGGGCATGCCGTGGGCTTGCCGTTCG
ACGCCATGCTCTCGAGCCTGCGCACCTTCGGCGGCCTCGAGCATCGCTGCCAGTGGGTGCGTGACCTCGACGGTGTCAGC
TATTACAACGATTCCAAGGCCACCAACGTCGGTGCGGCCCTGGCTGCCATCGAAGGCCTTGGCGCCGATATTGAAGGCAA
GCTGGTGCTGATCGCCGGTGGCGACGGCAAGGGCGCCGACTTCAAGGACCTTAAAGGCCCGGTGGCCACGCATTGCCGTG
CCGTGGTGCTGATCGGGCGCGATTCTGACTTGATCGCCGCCGCCCTGGGCGATGCCGTGCCGCAAGTCCGCGCCACGTCG
CTGGACGACGCCATCGCCCACTGCAAAGCCCTGGCCCAGCCGGGCGATGCGGTGCTGCTGTCGCCGGCGTGCGCCAGCTT
CGACATGTTCAAGAACTACGAAGAGCGCGGCCAGCTGTTCGCCCGCGCCGTGGAGGCCTTGGCATGA

Protein sequence :
MSLIASDHFRIVVGLGKSGMSLVRFLANRGKPFAVADTRENPPELVTLRRDYPHVEVRCGELDVEFLCRADELYVSPGLA
LATPALQAAAARGVKLSGDIDLFARHAKAPIVAISGSNAKSTVTTLVGEMAVAAGKRVAVGGNLGTPALDLLSDDIELYV
MELSSFQLETTHDLGAEVATVLNVSEDHMDRYSGLPAYHLAKHRIFRGAKQVVVNRQDALSRPLMGEGLPCWTFGLSKPD
FKAFGIREEHGEKYLAFEFQNLMPVRELKIRGAHNQSNALAALALGHAVGLPFDAMLSSLRTFGGLEHRCQWVRDLDGVS
YYNDSKATNVGAALAAIEGLGADIEGKLVLIAGGDGKGADFKDLKGPVATHCRAVVLIGRDSDLIAAALGDAVPQVRATS
LDDAIAHCKALAQPGDAVLLSPACASFDMFKNYEERGQLFARAVEALA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
BJAB07104_00311 YP_008207776.1 UDP-N-acetylmuramoylalanine-D-glutamate ligase Not tested AbaR25 Protein 5e-80 54
BJAB0868_00315 YP_008211641.1 UDP-N-acetylmuramoylalanine-D-glutamate ligase Not tested AbaR26 Protein 5e-80 54