Gene Information

Name : pykA (CLJ_B3675)
Accession : YP_002864383.1
Strain : Clostridium botulinum 657
Genome accession: NC_012658
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 3695402 - 3697159 bp
Length : 1758 bp
Strand : -
Note : identified by match to protein family HMM PF00224; match to protein family HMM PF00391; match to protein family HMM PF02887; match to protein family HMM TIGR01064

DNA sequence :
ATGCAAAAGACTAAAATGATATTTACAATAGGTCCTGCAAGCTCGACAGAAGAAATTGTATCAAAGTTAATAGAAGCTGG
AATGAGTGTATCAAGACACAATTTTTCTCACGGATCACATCCAGAACACAAAGAAAGAATGATGATGATTAAGAAATTAA
GAGAAAAGCATAACAAACACATAGCTATCATGTTAGACACCAAAGGACCAGAAATAAGAACGGGTAATTTTTCAGTGGAT
AAAGTGGAATTAAAAGAAGGGGCAGAGTTTACAATATATTGTAGAGAAGACATAATAGGTGACGAAACAAAATGTTCTAT
TACTTATGATGAACTATATAAAGATGTAGTTAAAGGAAATAAAATACTGATAGATGATGGATTAGTAGAGTTAGAGGTTC
AATCAGTAGAAGATAATAAAATTCATACAGTAGTTAAAAATTCAGGAACTGTTTCAAATCACAAGGGAGTAAATGTTCCA
GGAGTTTCAGTATCACTTCCAGCAGTTACTGAAAAAGATATAGAAGATTTAAAATTTGGATGTGAAGTAGGAGTAGATTT
AATAAGTGCATCCTTTATAAGAAAAGCCTCTGATGTATTAGCTATAAGAAAAATATTAGAAGAAAATGGTGGAAATGAAA
TACAGATAATCTCTAAGATAGAAAATCAAGAGGGCGTTGACAATATAGATGAGATAATAAAATTTTCTGATGGAATAATG
GTAGCTAGAGGAGATATGGGAGTTGAAATTCCTATAGAAGAGGTTCCAATAGTACAAAAAAGAATAATAGAAAAATGTAA
TAAGGCAGGTAAGCCTGTAATTACAGCAACTCAAATGTTAGATTCTATGATGAGAAACCCTAGACCAACAAGAGCTGAAG
CCTCAGACATAGCCAATGCAATATTTGATGGAACAGACGCCATAATGTTAAGTGGAGAATCAGCAAATGGAAAATATCCG
GTAGAAGCTGCAAGAACTATGTCTAGAATAGCTAAAACAGCAGAAGCAAAATTAAATTATGATGCTATATTAAATAAAAT
GAGAGAAAGTCATATATTAAATGTTCCTAATGCTATAAGCTTATCAGCGTGTACTACAGCATCAGAATTAAATGCTACAG
CTATAATCACAGCAACTCAAAGCGGACATACTGCAAAAATGGTATCAAAATATAGACCACAATGCCCTATTATAGCAGTA
ACACCAAATGAAATAGTTGCTAGAAAATTAGCATTAAATTGGGGAGTAGTGCCACTATTAACAGAAACATTTAATTCTAC
AGATGAGCTTATAGATAAATCTGTTAATAAATCTTTAGAAGAAGGATATGTTAAAAATGGCGACTTAGTTGTTATAGCAG
GAGGAATACCAGTGTCTTACTCAGGAACAACTAATATGCTAAAAGTTCATATAGTAGGAGATATATTATCTCAAGGTAGA
GGCTCAGGAACTAGACCAACTTATGGAAATGTAAAAGTAGTAAAAAATCCTGCGGAGGCAGAAGATATAGTTGAAAGAGA
TGACATATTAGTAGTTAAAAATTTAGATAGAAGATATATAACTATTTTAGACAGAGTATCAGGCATAGTGGCTGAAGAAG
GTGGAATAACATCACACTTAGCTATAGAATGCATAGCAAGAGATATTCCATTTATATGTAATGCAGGTGGAGCTATGGAT
GTTTTAAAAACAGGAACATATGTAACACTTGATACAGTAAGAGGAATAGTATACAACGGAAGAGCTAATATAGTTTAA

Protein sequence :
MQKTKMIFTIGPASSTEEIVSKLIEAGMSVSRHNFSHGSHPEHKERMMMIKKLREKHNKHIAIMLDTKGPEIRTGNFSVD
KVELKEGAEFTIYCREDIIGDETKCSITYDELYKDVVKGNKILIDDGLVELEVQSVEDNKIHTVVKNSGTVSNHKGVNVP
GVSVSLPAVTEKDIEDLKFGCEVGVDLISASFIRKASDVLAIRKILEENGGNEIQIISKIENQEGVDNIDEIIKFSDGIM
VARGDMGVEIPIEEVPIVQKRIIEKCNKAGKPVITATQMLDSMMRNPRPTRAEASDIANAIFDGTDAIMLSGESANGKYP
VEAARTMSRIAKTAEAKLNYDAILNKMRESHILNVPNAISLSACTTASELNATAIITATQSGHTAKMVSKYRPQCPIIAV
TPNEIVARKLALNWGVVPLLTETFNSTDELIDKSVNKSLEEGYVKNGDLVVIAGGIPVSYSGTTNMLKVHIVGDILSQGR
GSGTRPTYGNVKVVKNPAEAEDIVERDDILVVKNLDRRYITILDRVSGIVAEEGGITSHLAIECIARDIPFICNAGGAMD
VLKTGTYVTLDTVRGIVYNGRANIV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 7e-105 51
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 5e-105 51
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 5e-105 51
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 4e-104 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykA YP_002864383.1 pyruvate kinase VFG0479 Protein 2e-105 51