Gene Information

Name : polA (CLJ_B3273)
Accession : YP_002864017.1
Strain : Clostridium botulinum 657
Genome accession: NC_012658
Putative virulence/resistance : Unknown
Product : DNA polymerase I
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0749
EC number : 2.7.7.7
Position : 3279067 - 3281694 bp
Length : 2628 bp
Strand : -
Note : has 3'-5' exonuclease, 5'-3' exonuclease and 5'-3'polymerase activities, primarily functions to fill gaps during DNA replication and repair

DNA sequence :
ATGAATAAAGAAAGATTATTAATATTAGATAGTAATAGTTTATTAAATAGAGCTTTTTATGCATTACCTGATCTTATGAC
TGGAGAGGGAATTCATACTAATGCTATATATGGTTTTGTTAATATGCTTTTAAAAATGAAGGAAGAGATAAAGCCAGATT
ATATAGTTGCAGCTTTTGATAGAAAGGCACCTACCTTTAGACATGAAGAATATAAGGACTATAAAGCAGGTAGAAAAAAA
ATGCCAGAGGAACTTGCACAGCAGTTTCCTATAGTAAAGGATCTTTTAGCCGCATTGGCTATAAATATATTCGAAATTGA
TGGATTTGAAGCAGACGATCTAATAGGAACTTTATCTGTATTTGCAGAGGAAAAAGGAATAGAAGTTTACATAGTTACAG
GAGATAAAGATGCTCTACAATTGGCTACGGACAACGTAAAAGTAGTTATAACTAAAAAGGGTATTACAGAAAAAGAAATT
TATGATAAAAATAGAATGATAGAGGAGTTTGGAGTTACTCCTAAAGAATTTATAGATGTAAAAGGTCTTATGGGAGATAC
CTCTGACAATATACCTGGAGTACCTGGCATAGGAGAAAAAACTGCCTTTAAGCTTATAAAAGAATATAAAAGTATAGAAA
CAGTACTTGAAAACATAGAGAATATAAAAGGGAAAAAATTAAAGGAGAATTTACATGAATATAGAGAGCAGGCTATATTT
AGCAAAAAATTAGCTACTATTATATGTAATGTTCCTATAGATATGAGTTTAGAAGATATAAAATCAAAGGAAGAATATGA
TATAAACAAAGTTAGAAAAATGTTTGAAAGATTAGAATTTAAATCTTTAATGAATAAAGTAGGCAAAAGTGATGAGGAAG
AAACTGTAGAAGAGGAAGTTTCTTATAAAAATATAGTTTCTCTAGAAGAATTTAAAGTTCTAAAAAATAATATAATAAAA
TATAAAGAAAATGATTTATATCTGTATTTTGAATTAGAAGATATAGTTTTATTTTCTAAATCTAGAATAAAAACATTATA
TGCTAATTTTAAAGATGAAGTTTATAGAATAGACTTTGAAATGTTGCTAAAGAATACTAAAGAACAATTTATAGAAGTAT
GTAAAGAAATATTTGAAAGTAAAGAAATTAAAAAAATTACCTATGATGCAAAAAATCCAAGGACTATACTAAGAAAATTA
GGAATAGAGTTTAATGGTATAAAATTTGATATAAATTTAGCTGCATATCTAATAGATCCAGTGAGGAAAGAATATGAGAT
ATCTAGTCTAGCTTCAGAATATTTGTTTAAAAATATAAACAGAGAAGATGAAATTTTAAAAATAAAAGAAGTTAATATGA
TGCCTAAGTTATATAAAGTGCTAGAAGAAAAAATAAAAGAGCAAGATATGGAAGAATTGCTATATAAGGTAGAACAACCT
CTTACAGAAACATTATCAGCTATGGAGACAGAAGGTTTTAAAGTTGATAAAGATATACTTCTACAGCTAGAGAAAAAATT
TAAAGGGGAAATAGAAAGTACTCAGCTAGAAATATATTCAATGTCTGAAGAAGAATTTAATATAAATTCTCCTAAACAGT
TAGGGAAAATACTCTTTGAAAAATTAGATTTACCCGTTATAAAGAAAACAAAGACAGGATATTCTACTAACGCAGAGGTA
CTGGATAAATTAAGAGATAAACATCCAATAATAGATAAAATAACTTATTATAGACAATTAACAAAAATATACTCTACTTA
TATAGAGGGGCTAAAAGCTGCTATAGATGAGGATGGAAAAATACATTCAAATTTTAATCAAACTGTTACAGCTACAGGAA
GATTATCTAGTACAGAGCCTAATCTTCAAAATATACCTATAAAATATGAAATGGGAAGAGAAATAAGAAAGGTATTTATC
CCTAATACAGGGGATTCTGTAATACTTTCAGCGGATTATTCACAAATAGAATTAAGAGTATTAGCACATATATCTGATGA
TAAAAATATGATAAGTGCCTTTAATGAACATGATGATATTCATACTAAAACTGCATCAGAAGTGTTTAAAGTTCCAATAG
AAGAAGTAACACCTCTTATGAGAGGTAATGCTAAAGCAGTTAATTTTGGTATAGTTTATGGTATAGGAGACTTTAGCTTA
TCTCAAGATTTAAATATAACTAGAAAAGAAGCAAAGCAGTATATAGATGCTTATTTGGAGAGATATCCTAATGTAAAACT
TTATTTAGAAAATATAGTAGAAGAAGCCGTTGAAAAAGGTTATGTAAATACTATATTAAATAGAAGAAGATATATAAAAG
AGGTTAAGTCTTCAAATAAAATAGTTAAAGCTGCAGGAGAAAGGCTAGCTATGAATAGTCCAATACAAGGAAGTGCAGCG
GATATAATAAAATTAGCTATGGTAAATGTTCATAGAAAATTAAAAGAAGATAATTTTAAAAGTAGTATAATATTACAAGT
ACATGATGAATTAATATTAAATGTTTATAAAGATGAACTAGAAAAAATCAAAGCTTTAGTAAAAAAAGAAATGGAACAGG
TTTTAGAATTAAAAGTTGGGCTAGACGTGGATATAAACATAGGTAATAATTGGTATGAAGCAAAGTAG

Protein sequence :
MNKERLLILDSNSLLNRAFYALPDLMTGEGIHTNAIYGFVNMLLKMKEEIKPDYIVAAFDRKAPTFRHEEYKDYKAGRKK
MPEELAQQFPIVKDLLAALAINIFEIDGFEADDLIGTLSVFAEEKGIEVYIVTGDKDALQLATDNVKVVITKKGITEKEI
YDKNRMIEEFGVTPKEFIDVKGLMGDTSDNIPGVPGIGEKTAFKLIKEYKSIETVLENIENIKGKKLKENLHEYREQAIF
SKKLATIICNVPIDMSLEDIKSKEEYDINKVRKMFERLEFKSLMNKVGKSDEEETVEEEVSYKNIVSLEEFKVLKNNIIK
YKENDLYLYFELEDIVLFSKSRIKTLYANFKDEVYRIDFEMLLKNTKEQFIEVCKEIFESKEIKKITYDAKNPRTILRKL
GIEFNGIKFDINLAAYLIDPVRKEYEISSLASEYLFKNINREDEILKIKEVNMMPKLYKVLEEKIKEQDMEELLYKVEQP
LTETLSAMETEGFKVDKDILLQLEKKFKGEIESTQLEIYSMSEEEFNINSPKQLGKILFEKLDLPVIKKTKTGYSTNAEV
LDKLRDKHPIIDKITYYRQLTKIYSTYIEGLKAAIDEDGKIHSNFNQTVTATGRLSSTEPNLQNIPIKYEMGREIRKVFI
PNTGDSVILSADYSQIELRVLAHISDDKNMISAFNEHDDIHTKTASEVFKVPIEEVTPLMRGNAKAVNFGIVYGIGDFSL
SQDLNITRKEAKQYIDAYLERYPNVKLYLENIVEEAVEKGYVNTILNRRRYIKEVKSSNKIVKAAGERLAMNSPIQGSAA
DIIKLAMVNVHRKLKEDNFKSSIILQVHDELILNVYKDELEKIKALVKKEMEQVLELKVGLDVDINIGNNWYEAK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
polA YP_281523.1 DNA polymerase I Not tested Not named Protein 4e-165 44