Gene Information

Name : fliI (NGR_c02720)
Accession : YP_002824823.1
Strain : Sinorhizobium fredii NGR234
Genome accession: NC_012587
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 313488 - 314888 bp
Length : 1401 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGGAACCCCAAGGCTCTGACACCATTCCAGCTTCGACCTCGCTGGCGGCGCTCGCCGGCCTTGTCGAGCGCTATGCCAA
TCCCGATGTTTCGATCGCCCCTGGCGGTCATGTCCAGACGATCTCGCCGGGGCACTATACCGTCAGCGGCCTGTCCCGGC
ATGTGCGGCTGGGGGATTTCGTGGCGCACAAAAGTGCCACCGGCACCCATCTCGGCGAAGTGGTCCGCGTCGAACCGGAT
CGGGTCGTCGTCTGCCCGATCGAGCCGGGCGATCCGATCGGCATCCATGACACCGTCATCCGCAAGGGCGCCTTCCGTAT
CGCCCCGACGGACAACTGGTGCGGCCGGACGATCAACGCGCTGGCCGAACCGATCGATGGCCGCGGCCCCCTCATGCAGG
GCGACATGCGTCGTTCGATCTCCAATACCGCGCCGCCGTCGATGACCCGCAGACGGGTCGAACAGGGTTTCAAGACCGGC
GTGCGCGCCGTCGACATCTTCTCGCCGCTCTGCCTCGGCCAGCGCCTCGGCATCTTTGCCGGCTCTGGTGTCGGCAAGTC
GACGCTGCTGTCGATGCTGGCGCGCGCCGACGCCTTCGACAAGGTGGTGATCGCGCTGGTCGGCGAACGCGGCCGCGAAG
TGCGTGAATTCATCGAGGATACGCTTGGTGACAATCTCGCCAAATCCGTTGCCGTCGTCGCCACCAGTGACGAGAGCCCG
ATGCTCCGGAAAATGGCGCCGCTGACGGCGGTGACGATCGCCGAACACTATCGCGACAAGGGCGACAACGTCCTCTTGAT
CGTCGACAGCGTGACGCGTTTTGCCCATGCGATCCGCGAGGTGGCGACAGCGGCCGGCGAGCCGCCGATCGCCCGCGGCT
ATCCGGCTTCGGTCTTTACCGAATTGCCGCGCCTGCTCGAACGCGCCGGCCCTGGCGCCGAGGGGACCGGGACGATCACC
GCGATCATCTCGATCCTGGTCGACGGCGACAATCACAACGATCCGGTTGCCGATTCGGCACGCGGCATCCTCGACGGCCA
CATCGTCCTCGACCGCAGCCTGGCCGAAGAGGGGCGCTATCCGCCGATCGACCCGCTGGCCTCCATCTCGCGCCTGGCAA
GGAAAGCCTGGACGCCGGATCAGGAAAAGCTCGTGGCGCGGCTGAAATCGCTCATCCATCGCTTCGAGGAGACCCGCGAC
CTCAGGCTGATCGGCGGCTACCGCGCCGGCGGCGACCCGGACCTCGACATGGCGATCAAACAGGTGCCCGTCATCTATGA
CGTCCTCAAGCAGACCCCTGGCGAGCGATCCGTCGCCGATGCCTTCACCGACCTTGCAAACGCGCTGAAGGCAGCCGCCA
TGGGTAACGCGCCCGCCGCGGCTATCCGGGGGAAGGGGTAG

Protein sequence :
MEPQGSDTIPASTSLAALAGLVERYANPDVSIAPGGHVQTISPGHYTVSGLSRHVRLGDFVAHKSATGTHLGEVVRVEPD
RVVVCPIEPGDPIGIHDTVIRKGAFRIAPTDNWCGRTINALAEPIDGRGPLMQGDMRRSISNTAPPSMTRRRVEQGFKTG
VRAVDIFSPLCLGQRLGIFAGSGVGKSTLLSMLARADAFDKVVIALVGERGREVREFIEDTLGDNLAKSVAVVATSDESP
MLRKMAPLTAVTIAEHYRDKGDNVLLIVDSVTRFAHAIREVATAAGEPPIARGYPASVFTELPRLLERAGPGAEGTGTIT
AIISILVDGDNHNDPVADSARGILDGHIVLDRSLAEEGRYPPIDPLASISRLARKAWTPDQEKLVARLKSLIHRFEETRD
LRLIGGYRAGGDPDLDMAIKQVPVIYDVLKQTPGERSVADAFTDLANALKAAAMGNAPAAAIRGKG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
escN AAC31513.1 L0034 Virulence LEE Protein 4e-59 45
escN NP_290268.1 hypothetical protein Virulence LEE Protein 6e-59 45
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 4e-59 45
ECs4568 NP_312595.1 EscN Virulence LEE Protein 6e-59 45
escN AAC38383.1 EscN Virulence LEE Protein 3e-59 45
escN YP_003236088.1 translocator EscN Virulence LEE Protein 6e-59 45
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-59 45
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-50 45
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 6e-51 45
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 4e-58 44
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-58 44
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-59 44
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-59 44
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-59 44
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 2e-48 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_002824823.1 flagellum-specific ATP synthase VFG0192 Protein 9e-62 48
fliI YP_002824823.1 flagellum-specific ATP synthase VFG2144 Protein 6e-65 47
fliI YP_002824823.1 flagellum-specific ATP synthase VFG0812 Protein 3e-59 45
fliI YP_002824823.1 flagellum-specific ATP synthase VFG0730 Protein 2e-59 45
fliI YP_002824823.1 flagellum-specific ATP synthase VFG0515 Protein 1e-59 44