Gene Information

Name : lysS (BAMEG_0089)
Accession : YP_002812760.1
Strain : Bacillus anthracis CDC 684
Genome accession: NC_012581
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 80424 - 81923 bp
Length : 1500 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGGATAACATGAACCACGAAGAATTAAACGACCAATTGCTTGTTCGTCGTGAAAAGCTACATAACTTACGTGAGCAAGG
AATCGATCCTTTCGGTAAACGATTTGAACGTACAAATGCAACAAATGACTTATTAAGCCTATATGGAGAGTTCTCTAAAG
AAGAATTAGAAGAGAAAGAAATCTCTGTTTCTATCGCTGGTCGTATTATGACAAAGCGTGGTAAAGGAAAAGCTGGATTT
GCACATATTCAAGATTTACATGGACAAGTTCAAATTTATGTTCGTAAAGATGCTGTTGGAGATGAAGAATACGAATTATT
TAAAACAGCAGATTTAGGTGACTTAGTAGGTATTGAAGGTAAAGTTTTTAAAACGAACGTTGGAGAACTTTCAGTGAAAG
CAACAGGCTTTACACTATTAACGAAATCTCTTCGTCCATTACCGGATAAATATCATGGGTTAAAAGATGTTGAACAACGC
TACCGTCAACGTTACTTAGACTTAATTACAAGTATGGAAAGTCGTGAAACATTCGTCACTCGCAGTAAGATCATTCGCGA
AATGAGAAGATACTTAGATGACAACGGTTATCTTGAAGTGGAAACACCTATGATGCATGCAATTGCAGGTGGAGCTTCTG
CTCGTCCTTTCATCACGCATCATAATGCGTTAGATATGGAATTATATATGCGTATCGCAATTGAACTTCATTTAAAACGC
CTTATTGTGGGTGGATTAGAAAAAGTTTATGAAATCGGCCGTGTATTCCGTAATGAGGGTGTATCAACTCGTCATAATCC
TGAATTTACGATGATTGAATTATATGAAGCGTATGCTGATTATAAAGATATTATGAAACTAACAGAAAATATGGTTGCTC
ATATCGCGAAGCAAGTACTAGGTACAACAACAATCCAATATGGTGATTATGAAATTAATCTAGAACCAGAATGGACACGC
CTTCATATGGTAGATGCAATTAAAGAACATTCTGGAGCAGATTTCTGGAATCCGATGAGTGTAGAAGAAGCGCGTGAACT
TGCAAAAGAACATAATGTAGAAATTAAGGATACAATGGAAGTTGGTCATATTATTAATGAATTCTTCGAGCAAAAAGTAG
AAGATAAATTAATCCAACCAACATTTATTTACGGTCATCCGGTAGAAATTTCGCCACTTGCGAAAAAGAATGATGAAGAT
CCACGATTCACAGATCGTTTCGAATTGTTTATCGTTGCACGTGAACATGCAAATGCGTTCACTGAGCTAAATGATCCAAT
CGATCAGAAAGAACGTTTTGAAGCGCAATTAAAAGAACGCGAACAAGGTAATGACGAAGCTCACATGATGGATGATGATT
ATATCGAAGCTCTTGAGTATGGTATGCCTCCTACAGGTGGACTAGGAATTGGTATTGATCGCCTTGTTATGTTATTAACG
AATGCACCATCTATTCGTGATGTATTATTATTCCCTGCTATGCGTCATAAACAAGACTAA

Protein sequence :
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTNATNDLLSLYGEFSKEELEEKEISVSIAGRIMTKRGKGKAGF
AHIQDLHGQVQIYVRKDAVGDEEYELFKTADLGDLVGIEGKVFKTNVGELSVKATGFTLLTKSLRPLPDKYHGLKDVEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFITHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTENMVAHIAKQVLGTTTIQYGDYEINLEPEWTR
LHMVDAIKEHSGADFWNPMSVEEARELAKEHNVEIKDTMEVGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDED
PRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NAPSIRDVLLFPAMRHKQD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-121 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_002812760.1 lysyl-tRNA synthetase VFG1668 Protein 1e-121 51