Gene Information

Name : polA (CLM_3411)
Accession : YP_002805528.1
Strain : Clostridium botulinum Kyoto
Genome accession: NC_012563
Putative virulence/resistance : Unknown
Product : DNA polymerase I
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0749
EC number : 2.7.7.7
Position : 3440204 - 3442831 bp
Length : 2628 bp
Strand : -
Note : has 3'-5' exonuclease, 5'-3' exonuclease and 5'-3'polymerase activities, primarily functions to fill gaps during DNA replication and repair

DNA sequence :
ATGAATAAAGAAAGATTATTAATATTAGATAGTAATAGTTTATTAAATAGAGCTTTTTATGCATTACCTGATCTTATGAC
TGGAGAGGGAATTCATACTAATGCTATATATGGTTTTGTTAATATGCTTTTAAAAATGAAAGAAGAGATAAACCCAGATT
ATATAGTTGCAGCTTTTGATAGAAAGGCACCTACCTTTAGACATGAAGAATATAAGGACTACAAAGCAGGTAGAAAAAAG
ATGCCAGAGGAACTTGCACAGCAGTTTCCTATAGTAAAAGAGCTTTTGTCCGCATTAGCCATAAATATATTCGAAATTGA
TGGATTTGAAGCAGACGATCTAATAGGAACTTTATCTGTATTTGCAGAGGAAAAAGGAATAGAAGTTTACATAGTTACAG
GAGATAAAGATGCCCTACAATTGGCTACAGATAATGTAAAAGTAGTTATAACTAAAAAGGGTATTACAGAAAAAGAAATT
TATGATAAAAATAGAATGATAGAAGAGTTTGGAGTTACTCCTAAAGAATTTATAGATGTAAAAGGTCTTATGGGAGACAC
ATCTGACAATATACCTGGAGTACCTGGAATAGGTGAAAAAACCGCCTTTAAGCTTATAAAAGAATATAAAAGTATAGAAA
CAGTACTTGAAAACATAGAGAATATAAAAGGGAAAAAATTAAAGGAAAATTTACATGAATATAGAGAACAGGCTATATTT
AGCAAAAAATTAGCTACTATTATATGTAATGTTCCTATAGATATGAGTTTAGAAGATATAAAATCAAAGGAAGAATATGA
TATAAACAAAGTTAGAAAAATGTTTGAAAGGTTAGAATTTAAATCTTTAATGAATAAAGTAAGCAAAAGTGATGAGGAAG
AAACTGTAGAAGAGGAAGTTTCTTATAAAAATATAGTTTCTCTAGAAGAATTTAAAGATCTAAAAAATAATATAATAAAA
TATAAAGAAAATGATTTATATCTGTATTTTGAATTAGAAGATATAGTTTTATTTTCTAAATCTAGAGTAAAAACATTATA
TGCTAATTTTAAAGATGAAGTTTATAGAATAGACTTTGAAATGTTGCTAAATAATACTAAAGAACAATTTATAGAAGTAT
GCAGGGAAATGTTTGAGAGTAAAGAAATTAAAAAAATTACCTATAATGCAAAAAATCCAAGGACTATACTAAGAAAATTA
GGAATAGATTTTAATGGTATAAAATTTGATATAAATTTAGCTGCATACCTAATAGATCCAGTGAGGAAAGAATATGAGAT
ATCTAGTTTAGCTTCAGAATATTTGTTTAAAAATATAAATAGAGAAGATGAATTTTTAAAAATAAAAGAAGTTAATATAA
TGCCTAATTTATATAAAGTGCTGGAAGAAAAAATAAAAGATCAAGATATGGAAGAATTGCTATATAAGGTAGAACAACCT
CTTACAGAAACATTATCAGCTATGGAAACAGAAGGTTTTAAAGTTGATAAAGATATACTTTTACAGCTAGAGAAAAAATT
TAAAGGGGAAATAGAAAGTACTCAGCTAGAAATATATTCAATGTCTGAAGAAAAATTTAATATAAATTCTCCTAAACAGT
TAGGGAAAATACTCTTTGAAAAATTAGATTTACCTGTTATAAAGAAAACAAAGACAGGATATTCTACTAATGCAGAGGTA
CTGGATAAATTAAGAGATAAACATCCAATAATAGATAAAATAACTTATTATAGACAATTAACAAAAATATACTCTACTTA
TATAGAAGGGCTAAAAGCTGCTATAGATGAGGATGGAAAAATACATTCAAATTTTAATCAAACTGTTACAGCTACAGGGA
GATTATCTAGTACAGAGCCTAATCTTCAAAATATACCTATAAAATATGAAATGGGAAGAGAAATAAGAAAGGTATTTATC
CCTAATACAGGGGATTCTGTAATACTTTCAGCGGATTATTCACAAATAGAATTAAGAGTATTAGCACATATATCTGATGA
TAAAAATATGATAAGTGCATTTAATGAACATGATGATATTCATACTAAAACTGCATCAGAAGTGTTTAAAGTTCCAATAG
AAGAAGTAACACCTCTTATGAGAAGTAACGCTAAAGCAGTTAATTTTGGTATAGTTTATGGTATAGGAGACTTTAGCTTA
TCTCAAGATTTAAATATAACTAGAAAAGAAGCAAAGCAGTATATAGATGCTTATTTGGAGAGATATCCTAATGTAAAACT
TTATTTAGAAAATATAGTAGAAGAAGCCGTTGAAAAAGGGTATGTAAGTACTATATTAAATAGAAGAAGATATATAAAAG
AGGTTAAGTCTTCAAATAAAATAGTTAAAGCTGCAGGGGAAAGGCTAGCTATGAATAGTCCAATCCAAGGAAGTGCAGCA
GATATAATAAAATTAGCTATGGTAAATGTTCATAGAAAATTAAAAGAAGATAATTTTAAAAGTAGTATAATATTACAAGT
ACATGATGAATTAATATTAAATGTTCATAAAGATGAACTAGAAAGAATCAAAGCTTTAGTAAAAAAAGAAATGGAACAGG
TTTTAGAATTAAAAGTTGGGTTAGACGTGGATATAAACATAGGTAATAATTGGTATGAAGCAAAGTAG

Protein sequence :
MNKERLLILDSNSLLNRAFYALPDLMTGEGIHTNAIYGFVNMLLKMKEEINPDYIVAAFDRKAPTFRHEEYKDYKAGRKK
MPEELAQQFPIVKELLSALAINIFEIDGFEADDLIGTLSVFAEEKGIEVYIVTGDKDALQLATDNVKVVITKKGITEKEI
YDKNRMIEEFGVTPKEFIDVKGLMGDTSDNIPGVPGIGEKTAFKLIKEYKSIETVLENIENIKGKKLKENLHEYREQAIF
SKKLATIICNVPIDMSLEDIKSKEEYDINKVRKMFERLEFKSLMNKVSKSDEEETVEEEVSYKNIVSLEEFKDLKNNIIK
YKENDLYLYFELEDIVLFSKSRVKTLYANFKDEVYRIDFEMLLNNTKEQFIEVCREMFESKEIKKITYNAKNPRTILRKL
GIDFNGIKFDINLAAYLIDPVRKEYEISSLASEYLFKNINREDEFLKIKEVNIMPNLYKVLEEKIKDQDMEELLYKVEQP
LTETLSAMETEGFKVDKDILLQLEKKFKGEIESTQLEIYSMSEEKFNINSPKQLGKILFEKLDLPVIKKTKTGYSTNAEV
LDKLRDKHPIIDKITYYRQLTKIYSTYIEGLKAAIDEDGKIHSNFNQTVTATGRLSSTEPNLQNIPIKYEMGREIRKVFI
PNTGDSVILSADYSQIELRVLAHISDDKNMISAFNEHDDIHTKTASEVFKVPIEEVTPLMRSNAKAVNFGIVYGIGDFSL
SQDLNITRKEAKQYIDAYLERYPNVKLYLENIVEEAVEKGYVSTILNRRRYIKEVKSSNKIVKAAGERLAMNSPIQGSAA
DIIKLAMVNVHRKLKEDNFKSSIILQVHDELILNVHKDELERIKALVKKEMEQVLELKVGLDVDINIGNNWYEAK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
polA YP_281523.1 DNA polymerase I Not tested Not named Protein 2e-165 45