Gene Information

Name : fliI (CLM_3025)
Accession : YP_002805163.1
Strain : Clostridium botulinum Kyoto
Genome accession: NC_012563
Putative virulence/resistance : Virulence
Product : flagellar protein export ATPase FliI
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 3023866 - 3025182 bp
Length : 1317 bp
Strand : -
Note : identified by match to protein family HMM PF00006; match to protein family HMM PF02874; match to protein family HMM TIGR01026; match to protein family HMM TIGR03496; match to protein family HMM TIGR03497

DNA sequence :
ATGTCCATGTTAGATATAGATTTTGAGAAAATAGAAAAAAGAATTAAAGAAACTAATTTTAACTACATGGAAGGTACCGT
TAGAAAAGTTATAGGATTAACTGTAGAAGTTGAAGGGATAAAAGCTTTTGTAGGAGAGGTTTGTATTATATATAACGAGA
AAAATCAAACAATTCCTTGTGAAGTAGTTGGCTTTAAAGAAGAAAATGTTATATTGATGCCTTTAGGAGAGCTTATAGGT
ATAGCTCCAGGATGCAAAGTGATACCTCAAGGAAAACCTCTTAGTGTTATATGCTCAGAGAATATATTAGGAAAAGTTTT
AGATGGACTTGGTAATCCACTGGAGGGAGAAGAATTAATGGAGGGTAGTCCATATGCTTTGGACAGCGACCCTCCAGATC
CTATGAAAAGAAGAAGAATAAAAGATGTTATACCTACGGGAATAAAAGCCATAGATGGATTTATAACTGTAGGGGAAGGA
CAGAGAATAGGTATATTTGCAGGTAGTGGAGTGGGTAAGAGTACTACTTTAGGTATGATTGCAAAATATGCAGAAGCAGA
TATAAACGTTATAGCTCTAATAGGAGAAAGAGGTAGAGAAGTAAGGGATTTTATAGAAAAAGATTTAGGAGAAGAAGGCC
TTAAAAAGTCTGTTATAGTTTGTGCTACCTCTGATAAGCCTGCATTAGTTAGATTAAAGGGGGCCTTTACAGCTACAGCT
ATAGCGGAATATTTTAGAGACCAAGGGAAAAAGGTAATTCTTATGATGGATTCTGTAACAAGGTTTGCCATGGCTCAAAG
AGAAATAGGACTAGCTATAGGGGAGCCGCCGGCAACCAAAGGTTATACACCTTCAGTATTTGCAAAGTTACCAAGGCTTA
TGGAAAGATCAGGTATGTCTAACAAAGGATCTATAACAGCCTTTTATACAGTGCTAGTTGATGGAGATGATTTTAATGAA
CCTATTGCAGATGCTGTAAGAGGTATATTAGATGGACATATAGTTTTATCTAGAAGTTTAGCTGCTAAAAACCATTATCC
AGCTATAGATATTTTAAGTAGTGTAAGCAGACTTATGTCAGAAATTGCTTCAAAGGAACATAAGAATGCAGCGGGAATGG
CAAGAGATCTTTTGGCTACTTATAAAAACTCAGAGGATCTTATAAACATAGGAGCTTATGTTAAGGGAAGTAATCCTAAA
GTTGATATGGCTATACATTATCATGAATCAATACTATCTTTCTTAAAACAGGGTATTAGTGAGCAACATTCCTTTGAGGA
AAGTACATCTATTCTTCAAGATATGTTTAAAAATTAA

Protein sequence :
MSMLDIDFEKIEKRIKETNFNYMEGTVRKVIGLTVEVEGIKAFVGEVCIIYNEKNQTIPCEVVGFKEENVILMPLGELIG
IAPGCKVIPQGKPLSVICSENILGKVLDGLGNPLEGEELMEGSPYALDSDPPDPMKRRRIKDVIPTGIKAIDGFITVGEG
QRIGIFAGSGVGKSTTLGMIAKYAEADINVIALIGERGREVRDFIEKDLGEEGLKKSVIVCATSDKPALVRLKGAFTATA
IAEYFRDQGKKVILMMDSVTRFAMAQREIGLAIGEPPATKGYTPSVFAKLPRLMERSGMSNKGSITAFYTVLVDGDDFNE
PIADAVRGILDGHIVLSRSLAAKNHYPAIDILSSVSRLMSEIASKEHKNAAGMARDLLATYKNSEDLINIGAYVKGSNPK
VDMAIHYHESILSFLKQGISEQHSFEESTSILQDMFKN

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-106 54
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-105 52
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 1e-105 52
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 4e-86 52
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-106 51
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-106 51
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-106 51
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-105 51
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-87 51
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-87 51
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-87 51
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 4e-87 51
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-87 51
escN YP_003236088.1 translocator EscN Virulence LEE Protein 9e-85 51
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 6e-85 51
escN NP_290268.1 hypothetical protein Virulence LEE Protein 8e-85 51
escN CAI43874.1 EscN protein Virulence LEE Protein 6e-85 51
ECs4568 NP_312595.1 EscN Virulence LEE Protein 8e-85 51
escN YP_003223475.1 translocator EscN Virulence LEE Protein 8e-85 51
escN AAK26715.1 EscN Virulence LEE Protein 1e-84 51
escN AAL57542.1 EscN Virulence LEE Protein 1e-84 51
escN CAC81862.1 EscN protein Virulence LEE II Protein 1e-84 51
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-84 51
escN AAC31513.1 L0034 Virulence LEE Protein 6e-85 51
unnamed AAL06369.1 EscN Virulence LEE Protein 4e-83 51
escN AAC38383.1 EscN Virulence LEE Protein 5e-85 51
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-105 50
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-105 50
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-105 50
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-83 50
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-83 50
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 5e-80 47
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 4e-80 47
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 6e-79 46
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 7e-77 46
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 2e-82 46
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 2e-82 46
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 8e-83 46
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 5e-83 46
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 1e-87 46
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 3e-79 45
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 2e-77 44
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 2e-77 44
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 2e-68 41
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-68 41
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 2e-68 41
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-68 41
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-68 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_002805163.1 flagellar protein export ATPase FliI VFG2144 Protein 6e-114 53
fliI YP_002805163.1 flagellar protein export ATPase FliI VFG0390 Protein 9e-106 53
fliI YP_002805163.1 flagellar protein export ATPase FliI VFG0192 Protein 8e-105 53
fliI YP_002805163.1 flagellar protein export ATPase FliI VFG0048 Protein 4e-102 52
fliI YP_002805163.1 flagellar protein export ATPase FliI VFG1254 Protein 7e-101 51
fliI YP_002805163.1 flagellar protein export ATPase FliI VFG0515 Protein 1e-87 51
fliI YP_002805163.1 flagellar protein export ATPase FliI VFG0812 Protein 3e-85 51
fliI YP_002805163.1 flagellar protein export ATPase FliI VFG0730 Protein 3e-85 51
fliI YP_002805163.1 flagellar protein export ATPase FliI VFG2331 Protein 6e-90 48
fliI YP_002805163.1 flagellar protein export ATPase FliI VFG2499 Protein 1e-91 48
fliI YP_002805163.1 flagellar protein export ATPase FliI VFG1778 Protein 2e-70 42
fliI YP_002805163.1 flagellar protein export ATPase FliI VFG2459 Protein 9e-72 41
fliI YP_002805163.1 flagellar protein export ATPase FliI VFG0555 Protein 9e-69 41
fliI YP_002805163.1 flagellar protein export ATPase FliI VFG1008 Protein 1e-64 41