Gene Information

Name : lysS (Avin_39390)
Accession : YP_002801054.1
Strain : Azotobacter vinelandii DJ
Genome accession: NC_012560
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 3988146 - 3989648 bp
Length : 1503 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGAGCGAACAACCGCTGAACCAGCATGCCCTCCACGAGGAAGAAAACAAGCTGATCGCCCTGCGCAAGGAGAAACTCGC
CGCCGAGCGCGCGAAAGGCAATGCCTTCCCCAACGACTTCCGCCGCGACAGCTACTGCGCCGACCTGCAGAAGCGCTATG
CCGAGGCGGGCAAGGAGGAGCTGGAAGCGGCCGCGATCCCGGTCAAAGTGGCCGGGCGCATCATGCTCAACCGCGGCGCC
TTCATGGTCATCCAGGACATGACCGGGCGCATCCAGGTCTACGTCGACCGCAAGGGCCTGCCGGCCGAGACGCTGGAGGC
GGTCAAGCACTGGGACCTGGGCGACATCATCGCCGCCGAGGGCACCCTGGCGCGCTCCGGCAAGGGCGATCTCTACGTGC
ACATGAGCACGGTGCGCCTGCTGACCAAGTCGCTGCGCCCGCTGCCGGACAAGCATCATGGCCTGACCGACACCGAGCAG
CGCTACCGCCAGCGTTACGTCGACCTGATGGTCAACGAGGAAACCCGCCACACCTTCCGCGTGCGTTCGCAGATCATCGC
CCACATTCGCAGGTTCCTCGCCGAGCGCGACTTCCTCGAGGTGGAAACGCCGATGCTCCAGGTCATCCCCGGCGGCGCCG
CGGCCAAGCCCTTCCAGACCCACCACAACGCGCTGGACCTGCCGATGTTCCTGCGCATCGCACCCGAGCTCTACCTCAAG
CGCCTGGTGGTCGGCGGCTTCGAGCGGGTCTTCGAGATCAACCGCAACTTCCGCAACGAAGGCGTCTCGACCCGCCACAA
CCCCGAATTCACCATGCTCGAGTTCTACCAGGCCTACGCCGACTACGAGGACAACATGGACCTCACCGAGCAGTTGTTCC
GCGAACTGGCGCAACTGGTGCTGGGGACCACCGACGTGCCCTACCAGGGTAAGGTGTTCCACTTCGGCGAGCCCTTCGCC
CGCCTGTCGGTGTTCGACTCGATCCTCTACTACAACCCGGAAGTCTCCGCCGCGGACCTGCGCGACATCGAGAAATGCCG
CGCCATCGCCAGGAAGGCCGGCGCCGACGTCAAGGGTTTCGAGGGACTGGGCAAGCTGCAGACGATGATCTTCGAGGAGT
TGGTCGAGCACCGCCTGGAACAGCCGACCTTCATCACCCGCTATCCCTTCGAGGTTTCGCCGCTGGCGCGGCGCAGCGAC
GACGATCCGAGCGTCACCGACCGCTTCGAGCTGTTCATCGGCGGCCGCGAGATCGCCAACGCCTACTCCGAGCTGAACGA
CGCCGAGGACCAGGCCGAGCGCTTCATGCAGCAGGTCCGGGACAAGGATGCCGGCGATGACGAGGCCATGCACTTCGACG
CCGACTTCGTGCGCGCCCTGGAATACGGCATGCCGCCGACCGCCGGCGAAGGCATCGGCATCGACCGTCTGGTGATGCTG
CTGACCGATTCGCCGTCGATCCGCGACGTGATCCTTTTCCCGCACATGCGTCCGGAAGTCTGA

Protein sequence :
MSEQPLNQHALHEEENKLIALRKEKLAAERAKGNAFPNDFRRDSYCADLQKRYAEAGKEELEAAAIPVKVAGRIMLNRGA
FMVIQDMTGRIQVYVDRKGLPAETLEAVKHWDLGDIIAAEGTLARSGKGDLYVHMSTVRLLTKSLRPLPDKHHGLTDTEQ
RYRQRYVDLMVNEETRHTFRVRSQIIAHIRRFLAERDFLEVETPMLQVIPGGAAAKPFQTHHNALDLPMFLRIAPELYLK
RLVVGGFERVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYEDNMDLTEQLFRELAQLVLGTTDVPYQGKVFHFGEPFA
RLSVFDSILYYNPEVSAADLRDIEKCRAIARKAGADVKGFEGLGKLQTMIFEELVEHRLEQPTFITRYPFEVSPLARRSD
DDPSVTDRFELFIGGREIANAYSELNDAEDQAERFMQQVRDKDAGDDEAMHFDADFVRALEYGMPPTAGEGIGIDRLVML
LTDSPSIRDVILFPHMRPEV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 6e-132 59
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 7e-78 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_002801054.1 lysyl-tRNA synthetase VFG1668 Protein 4e-132 59