Gene Information

Name : cadA-1 (LHK_00449)
Accession : YP_002794453.1
Strain : Laribacter hongkongensis HLHK9
Genome accession: NC_012559
Putative virulence/resistance : Resistance
Product : CadA-1
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : 3.6.1.-
Position : 418430 - 420628 bp
Length : 2199 bp
Strand : -
Note : Heavy metal-(Cd/Co/Hg/Pb/Zn)-translocating P-type ATPase:Heavy metal translocating P-type ATPase

DNA sequence :
ATGCACAACGACACACCGCTCAAGCTCCGTCCGGCGACCTCCGGCACCTCTTGTCAGGGAGGCTGCGTGTGCAGCAGCAC
CGCGCCTGTCCAGCCTGATCCTGCCCCCGCCGCCACTGACGGCCAGATCCTGTCACGCTGGCGCATCGACGAAATGGACT
GCCCGACCGAAGAAGGCCAGATCCGCCGGGCACTGGCCCGGCTGGACGGCGTCGGCGCCCTGTCCTTCAACCTGCTCAAG
CGCGAGCTGGCCGTCGCCCACGTCCCCGGGCAGGACGCCGCCATCATCGACGCCATCCGCGCGCTGGGCATGACACCGCA
GCCGCTGGACCAGCCCGCAGCCGCACCGGACACGGCCCGGCACTGGGGCTGGCTGGCACTGGCCATCGCACTGGCCCTGC
TGGCCGAAGGGCTGCACTGGTTCGGCGCGCCGGACGCGCTCGGGGCTGCGGCAGCCGTGGCCGCCATTGCTGCCGCCGGC
ACCTCGACCTACCGCAAGGGCTGGATTGCCCTGCGCCATGCCGACCTCAACATCCACGCCCTGATGACGCTGGCGGTGAC
CGGTGCCTTTGCCATCGGCCAGTGGCCGGAAGCCGCCATGGTCATGACCCTGTTTGCACTGGCCGAACGGCTGGAGGCAC
GCTCGCTCAGCCGCGCCCGCGATGCCATCGGCCGCCTGCTGACCCTGACGCCCGCTACCGCCACGGTCGAACGCGAGGGA
CAGTGGTGCAGCGTGCCGGCAGACAGCGTCACCCCCGGCGAGCGCCTGCGCGTGGCGCCAGGCGAACGGGTGGCACTGGA
CGCCCGTATCGAGGCCGGCCACAGCGCCCTGGACCAGTCCTCGCTGACCGGCGAGAGCCTGCCGGTTGACAAGGAGCCAG
GCGACAGCGTGTTTGCCGGCAGCATCAACCAGAGCGGCGAACTGGTCTGCCGGGTGACTGCCGTGGCCGGAGACTCGACC
CTGGCGCGGGTGATCCGCGCCGTGGAAGGCGCCCAGGCTGCGCGGGCGCCGATCCAGCAAACGGTGGACCGTTTTGCCCG
CATCTATACGCCGGTCGTGGTCGGACTGGCCGTGCTGCTGGCGCTGGTGCCGCCCCTCTTTGACGGCGGCTGGCTCGACT
GGCTGTACCGGGCGCTGGTGCTGCTGGTGATCGCCTGCCCGTGCGCGCTGGTGATCGCCACGCCGGTGACCATGGTCAGC
GCCCTGACCAACGCGGCCCGCCACGGCATTCTGGTGCGCGGCGGTGCGGTGCTCGACGCCGGACGCCGGCTGACCGTGAT
GGCGCTCGACAAGACCGGCACGCTGACCGCCGGCCGGCCGGTCGCCACCGACTTCGTGCCGCTGGCCGACGCAGGCGCCG
GGGAGCTGCGCAGCATTGCCGTCGCCCTCGCTGCCCGCTCCGACCACCCGGTGTCGCGCGCCATCGCCGCGGCGGCCCGG
CAGGACGGCCTGACGGCCACGGAAGCCGCCGGGCTGACGGCCCTGCCCGGTCGCGGCGTGCGCGGCGAGGTTGACGGACA
GGCCTACTGGCTCGGCAACCCGCGCCTGCTGGCCGAACTGGGCGTCAGCGTCCCGGCGGCCCGCATCGAAGCACTGGAAG
CCGGTGGCAAGACGGTGGTGACGCTCAGCGACGGCCGCCAGGCGCTGGCCCTGTTTGCCGTCGCCGATCCGGTGCGGCCG
GAAAGCCGCCAGGCGGTGGACGACCTGCACGCACTGGGCATCCGGACCGTCATGCTGACTGGCGACAACCCGCAGGCAGC
GCGCAGCGTGGCCACTACCCTCGGCATTGATGACGTACGCGCCGGCCTGCTGCCGGAAGACAAGCTCGCCGCCATCGAAG
GCCTGATGCAGTCTGCTGGCGCCCATGCCGGCATGGTGGGCGACGGCATCAACGATGCGCCGGCGCTGGCACGGGCGCAG
GTGGGCTTTGCCATGGGTGCGGCCGGCAGCGATACCGCCATCGAAACCGCCGACGTGGCGCTGATGGACGACGATCCGCG
CAAGCTGGCGCGCTTTGTCCGGCTGGCGCGTCAGGCCGGCCGGGTGCTGCGGCAGAACCTGGTGCTGGCGCTGGGCATCA
AGCTGGCGTTCCTGGCCCTGACGGTGGCGGGGCTGGGCAGCATGTGGATGGCGGTGTTTGCCGACGTCGGCGCCAGCCTC
TTGGTGATCGCCAACGGCCTGCGCCTGCTGCGCGCCTGA

Protein sequence :
MHNDTPLKLRPATSGTSCQGGCVCSSTAPVQPDPAPAATDGQILSRWRIDEMDCPTEEGQIRRALARLDGVGALSFNLLK
RELAVAHVPGQDAAIIDAIRALGMTPQPLDQPAAAPDTARHWGWLALAIALALLAEGLHWFGAPDALGAAAAVAAIAAAG
TSTYRKGWIALRHADLNIHALMTLAVTGAFAIGQWPEAAMVMTLFALAERLEARSLSRARDAIGRLLTLTPATATVEREG
QWCSVPADSVTPGERLRVAPGERVALDARIEAGHSALDQSSLTGESLPVDKEPGDSVFAGSINQSGELVCRVTAVAGDST
LARVIRAVEGAQAARAPIQQTVDRFARIYTPVVVGLAVLLALVPPLFDGGWLDWLYRALVLLVIACPCALVIATPVTMVS
ALTNAARHGILVRGGAVLDAGRRLTVMALDKTGTLTAGRPVATDFVPLADAGAGELRSIAVALAARSDHPVSRAIAAAAR
QDGLTATEAAGLTALPGRGVRGEVDGQAYWLGNPRLLAELGVSVPAARIEALEAGGKTVVTLSDGRQALALFAVADPVRP
ESRQAVDDLHALGIRTVMLTGDNPQAARSVATTLGIDDVRAGLLPEDKLAAIEGLMQSAGAHAGMVGDGINDAPALARAQ
VGFAMGAAGSDTAIETADVALMDDDPRKLARFVRLARQAGRVLRQNLVLALGIKLAFLALTVAGLGSMWMAVFADVGASL
LVIANGLRLLRA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ORF C97 AAN62190.1 putative metal-transporting P-type ATPase Not tested PAGI-2(C) Protein 2e-161 55
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 1e-109 44
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 1e-109 44
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 5e-109 42

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
cadA-1 YP_002794453.1 CadA-1 BAC0298 Protein 9e-160 57
cadA-1 YP_002794453.1 CadA-1 BAC0455 Protein 2e-84 43
cadA-1 YP_002794453.1 CadA-1 BAC0092 Protein 1e-76 42
cadA-1 YP_002794453.1 CadA-1 BAC0461 Protein 9e-87 42
cadA-1 YP_002794453.1 CadA-1 BAC0124 Protein 9e-71 42
cadA-1 YP_002794453.1 CadA-1 BAC0100 Protein 6e-57 41