Gene Information

Name : pyk (ROP_07330)
Accession : YP_002777925.1
Strain : Rhodococcus opacus B4
Genome accession: NC_012522
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 766677 - 768095 bp
Length : 1419 bp
Strand : -
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
GTGAACCGACGCACGAAGATCGTATGCACACTTGGACCTGCTACCGCCACCGGTGACCGTATTCGCGAGCTCGTCGAGAG
CGGTATGGATGTGGCGCGGCTGAATTTCAGCCACGGCGAGCACGCCGATCACGAGGAGAACTACAAACGTGTGAGGGCGG
CCTCGGACGCGACGGGTAAGGCGGTCGGTGTCCTGGCCGACCTGCAGGGGCCGAAGATCCGGTTGGGCCGGTTCGCGGAG
GGGCGCACCACGTGGGCGAACGGTGAGGAGGTGCGGATCACCGTCGAGGAGTGTGAGGGCACCCACGACCGGGTCTCGAC
CACGTACAAGCAGCTGGCCGAGGACGCCAAGCCGGGGGACCGGTTGCTGGTGGACGACGGCAAGGTCGGTCTGGTGGTCT
CCGGGGTGGAGGGCAATGACGTGATCTGCCGGGTCACCGAGGGCGGCCCGGTGTCGAACAACAAGGGTGTGTCGCTGCCG
GGGATGAATGTGTCGGTTCCGGCGCTGTCGGAGAAGGACATCGCGGATCTGGAGTTCGCGCTCGGGTTGGGTGTCGATTT
CATCGCGTTGTCGTTCGTGCGGTCGCCGGCGGATGTGGAGTTGGTGCATGCGGTGATGGACCGGGTGGGTCGGCGGATTC
CGGTGATCGCGAAGTTGGAGAAGCCGGAGGCGATCGACAATCTCGAGGCGATCGTGCTGGCGTTCGATGCGGTGATGGTG
GCGCGGGGTGATCTGGGTGTGGAGTTGCCGCTCGAGCAGGTGCCGTTGGTGCAGAAGCGGGCGATTCAGATTGCGCGGGA
GAACGCGAAGCCGGTGATCGTGGCGACGCAGATGCTGGAGTCGATGATCGAGAACTCGCGGCCGACCCGGGCGGAGGCCT
CGGATGTGGCGAACGCGGTCCTCGACGGGGCGGATGCGGTGATGCTGTCCGGGGAGACGTCGGTGGGTAAGTACGTGATG
GAGACGGTGCGCACGATGGCGCGGATCGTGGAGGCGGTGGAGAACGAGTCCACGCAGGTGCCGCCGCTGACGCATGTGCC
GCGGACCAAGCGGGGGGTGATCTCGTACGCGGCCCGCGACATCGGGGAGCGCCTGGATGCGAAGGCGTTGGTGGCGTTCA
CGCAGTCGGGGGACACGGTGCGCCGGTTGGCGCGGTTGCATACGCCGTTGCCGTTGCTGGCGTTCACGCCGTTGCCGGAG
GTGCGCAGCCAGTTGTCGTTGACGTGGGGGACGGAGACGTTCATCGTCGACCCGGTCGACAGCACGGATGCGATGGTGCG
GCAGGTCGACAACGCGCTGCTCGGGTTGGGCCGGTACCAGAAGGGTGAACTCGTGGTGATCGTGGCGGGGTCGCCGCCGG
GCACCGTAGGCTCGACCAACCTGATCCACGTCCACAGAATCGGCGAAGAGGACCACTAG

Protein sequence :
MNRRTKIVCTLGPATATGDRIRELVESGMDVARLNFSHGEHADHEENYKRVRAASDATGKAVGVLADLQGPKIRLGRFAE
GRTTWANGEEVRITVEECEGTHDRVSTTYKQLAEDAKPGDRLLVDDGKVGLVVSGVEGNDVICRVTEGGPVSNNKGVSLP
GMNVSVPALSEKDIADLEFALGLGVDFIALSFVRSPADVELVHAVMDRVGRRIPVIAKLEKPEAIDNLEAIVLAFDAVMV
ARGDLGVELPLEQVPLVQKRAIQIARENAKPVIVATQMLESMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKYVM
ETVRTMARIVEAVENESTQVPPLTHVPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVRRLARLHTPLPLLAFTPLPE
VRSQLSLTWGTETFIVDPVDSTDAMVRQVDNALLGLGRYQKGELVVIVAGSPPGTVGSTNLIHVHRIGEEDH

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 1e-66 41
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 5e-67 41
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 5e-67 41
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 1e-66 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk YP_002777925.1 pyruvate kinase VFG0479 Protein 2e-67 41