Gene Information

Name : metB (ROP_59060)
Accession : YP_002783098.1
Strain : Rhodococcus opacus B4
Genome accession: NC_012522
Putative virulence/resistance : Resistance
Product : cystathionine gamma-synthase
Function : -
COG functional category : E : Amino acid transport and metabolism
COG ID : COG0626
EC number : 2.5.1.48
Position : 6460501 - 6461673 bp
Length : 1173 bp
Strand : +
Note : catalyzes the formation of cystathionine from L-cysteine and O-succinyl-L-homoserine

DNA sequence :
ATGAGTGAGCAGCGCAGCAAAGCGGACAACATCAGCTGGCAGGGGTTCTCCACCAAGGCCGTGCACGCAGGATTCGACCC
GGACCCGCAGACGGGTGCCGTCAACGTCCCCATCTACGCCAGTTCGACGTTCGCGCAGGACGGCGTCGGCGGCATGCGGA
ACGGATTCGAGTACGCCCGCACCGGCAACCCCACCCGACGACCGCTCGAGGCCAACCTCGCCGCCCTCGAATCGGGCACG
TACGGTCGCGCCTTCGGCTCGGGCATGGCCGCCACCGACTGCCTGCTCCGTGCCGCGCTCCGCCCCGGCGACCACCTCGT
CATCCCCAACGACGCCTACGGCGGAACGTTCCGCCTGATCGACAAGGTCTTCACCCAGTGGGGCATCGAGTACACGCCCG
CCGCGGTGTCCGACGTGGACGCGGTCCGGGCCGCGATCCGTCCCAACACCAAGCTCGTGTGGGTCGAGACGCCCACCAAC
CCGCTGCTCAACATCGGTGACATCGAGGCGCTGTCCGACATCGCGCACGAGGCCGGCGCGAAGATCGTCGTCGACAACAC
GTTCGCCTCGCCCTACCTGCAGCAGCCGCTGCAACTCGGCGCCGACATCGCGCTGCACTCGACCACCAAATACATCGGCG
GACACTCCGACGTCGTCGGCGGCGCCCTGATCACCAACGACGAAGAACTCGACACGGCGTTCGCGTTCCTGCAGAACGGT
GCCGGCGCGGTCCCCGGACCGTTCGACGCGTTCCTCACCCTCCGCGGCATCAAGACCCTCTCGCTGCGGATGGAGCGGCA
CAGCGACAACGCCGAGAAGGTCGTCGAACTGCTCACCGGGCACCCCGCGGTGTCGCAGGTGATCTACCCCGGACTCGAGT
CACACCCCGGCCACAAGATCGCCGAGAAGCAGATGCGCCGCTTCGGCGGCATGATCTCGGTCCGCCTCACCGGCGGCAAG
CAGGCCGCCCTGGACTTCTGCTCGCGCACCGAGATCTTCACCCTCGCCGAATCGCTCGGCGGCGTCGAATCACTGATCGA
GCACCCGGGCGCCATGACCCACGCCTCCACCGCAGGTTCGCTCCTCGAGGTCCCCGACGACCTGGTGCGCCTGTCCGTCG
GCATCGAGGACGCCGCCGACCTGGTGGGCGACATCGAGCAGGCGCTCAAGTAG

Protein sequence :
MSEQRSKADNISWQGFSTKAVHAGFDPDPQTGAVNVPIYASSTFAQDGVGGMRNGFEYARTGNPTRRPLEANLAALESGT
YGRAFGSGMAATDCLLRAALRPGDHLVIPNDAYGGTFRLIDKVFTQWGIEYTPAAVSDVDAVRAAIRPNTKLVWVETPTN
PLLNIGDIEALSDIAHEAGAKIVVDNTFASPYLQQPLQLGADIALHSTTKYIGGHSDVVGGALITNDEELDTAFAFLQNG
AGAVPGPFDAFLTLRGIKTLSLRMERHSDNAEKVVELLTGHPAVSQVIYPGLESHPGHKIAEKQMRRFGGMISVRLTGGK
QAALDFCSRTEIFTLAESLGGVESLIEHPGAMTHASTAGSLLEVPDDLVRLSVGIEDAADLVGDIEQALK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SAS0418 YP_042545.1 Cys/Met metabolism PLP-dependent enzyme Not tested vSa¥á Protein 2e-77 48
SAR0460 YP_039909.1 Cys/Met metabolism PLP-dependent enzyme Not tested vSa¥á Protein 2e-76 47
metB YP_281510.1 cystathionine beta-lyase Not tested Not named Protein 2e-58 43

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
metB YP_002783098.1 cystathionine gamma-synthase BAC0207 Protein 4e-65 42