Gene Information

Name : lysS (ROP_35510)
Accession : YP_002780743.1
Strain : Rhodococcus opacus B4
Genome accession: NC_012522
Putative virulence/resistance : Unknown
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 3850317 - 3853661 bp
Length : 3345 bp
Strand : -
Note : catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA

DNA sequence :
ATGTCAGCTAGCACCGAGACACACCACGCGTCCGAGGCGGCCGTCCCCACCGCACCACGGCCCCGACCCGCCCTCGGCTC
GAAGAGCGGGCGCCTGCACCAGGTTCCCCACATCGCGGGACTGATCCTGGGCGTCTTCTCGGTGCTCGTATTCCTGTGGA
GCATCTCCCCGGTGCTGCGCTACTACGTGCACGTGCCGCGGGAGTACATCGACACGTACTACTTCGACGCCCCCGACACC
AGCCTGTCGTGGGCGCTGGTAGTCGCGCTGCTCGCGGCGGCGCTGGCCAGCCGCAAACGCATCGCCTGGTGGCTGCTGAC
GATCTATCTGGTGCTGATCCTGATCACCAACGTGATCGTGAGCATCACCGACCGGAACGTGAACGCGATGGTCGCCGCCG
TGGTCCAGGTGGTGCTGATCGGCATCCTCGTCGCGGCCCGGCCCGAGTTCTACACCCGGGTCCGGCGCGGCGCCGGCTGG
AAGGCGCTGGGCGTGCTCATCGTCGGGCTCGCGATCGGCACTCTCGTCGGGTGGGGACTCGTCGAATTGTTCCCCGGCAC
CCTGCCGCAGGGCGAGCGATTCCTGTGGGCTCTCAACCGCGTGACCGCGCTGGCCGCCGCCGACAACGAACAGTTCAGCG
GACGACCGCACGGATTCGTCAACACCCTCCTCGGCCTGTTCGGCGCCATGGCCCTGCTCGCCGCCGTCATCACCCTGTTC
CGCGCCCAGCGCTCCCACAACGCGCTCACCGGCAACGACGAATCGGCGCTGCGCGGACTGCTGCTGCAGTACGGCGCGGA
CGACTCCCTCGGCTACTTCGCCACCCGCCGCGACAAGGCCGTCGTGTTCGCGCCGAGCGGGAAGGCGGCCATCACCTACC
GCGTCGAACTCGGCGTCTGCCTCGCCAGCGGTGATCCGATCGGCGACCCCGAGGCCTGGCCACACGCGATCGAGGCGTGG
CAGACGCTCGCGTCCCAATACGGTTGGGCCACCGCCGTGATGGGCGCCAGCGAAACCGGGGCCACCGCCTACAACAAGGC
CGGTCTCACCGTGCTCCAGCTCGGCGACGAGGCGATCCTCCGCACCCGCGAGTTCAACCTCAGCGGTCGCGACATGCGGC
AGGTCCGCCAGGCCGTCACCCGCGTCCGCCGCCAGGGCGTCACCGTCCGGATCCGCAGGCACCGCGACGTCCCCCCCGAG
GAAATGGCCGAGGCCATCCGGCTCGCGGACGCCTGGCGCGACACCGAGACCGAGCGCGGATTCTCGATGGCGCTCGGCCG
GCTCGGCGACCGCCTCGACGGCGACTGCCTGCTGGTGGAGGCCATCGCCGAGGACGGTGAGATCGACGGCATCCTGTCGC
TGGTTCCGTGGGGACCGACCGGTGTCTCGCTCGACCTGATGCGCCGCAAGCCGACCTCCCCCAACGGGGTCGTCGAACTG
ATGGTGTCCGAACTGGCCACCACCTCCGACCAATTCGGCATCACCAAGGTGTCGCTGAACTTCGCGGTGTTCCGCTCGGT
CTTCGAGGAGGGCTCCCGCATCGGGGCCGGACCCATTCTGCGAATCTGGCGCTCGATCCTCGTCTTCTTCTCCCGATGGT
GGCAGCTCGAGGCGCTCTACCGATCCAACGTGAAGTACCAGCCGGAGTGGGTGCCGCGGTTCCTCTGCTTCGACGACAAC
AGGGAACTGCTGCGCGTCGGATTCGCCTCGGCCGTCGCGGAAGGATTCGTGACGCTGCCGCGGTTCGGCCGTTCCGGGAC
CCACGACGCCATCGAGCACACCGGCCACCACGCCGCGGTTCCCGCCGCACTCGTCGCCGCCGAGGGATTGCACTCCGACG
GCAGCGCCCCCGGTGAGGGCCTCGCACCGACGGCGACCGGACCCAAACGTCCCGAGCAGGTCCGCGTCCGGCTCGACAAG
TTGACCGGCCTGGCGGAACAGGGCATCGACCCCTATCCGGTCGCGTACCCGCCCAGCCACACCGTGACCGAGGCAGTGGA
ATCCCCCGAGGGGACGACGGTCCGCATCGCGGGACGCCTGCTCCGGATCCGCGACTACGGCGGCGTCGTGTTCGCGGTGG
TCCGGGACTGGTCCGGAGACATCCAGGTCCTCGTCGACGAAGCCCGGGTGGGAACCGATCGGATCCGGGCGTTCGCCGCC
GAATTCGACCTCGGCGACCTCGTCGAGGTCGCGGGTGTCATCGGCTACAGCCGCCGGGGAGCGCTGTCGCTGCTGGCGAA
CGAGTGGCGCATGACCGGCAAGTGCCTGCACCCCCTGCCCGACAAGTGGAAGGGACTGTCCGACCCGGAGACCCGGGTGC
GGCAGCGGTACGTCGACCTCGCGATCAACACCGATGCCCGGCGCCTGCTCGAGGCCCGCAGCGCCGTCGTGAAGTCGTTG
CGGGATTCGCTCGGCGGCCGCGGCTTCCTCGAGGTGGAGACCCCGATCCTGCAGCAGGTCCACGGCGGCGCGAACGCGGC
CCCGTTCCTCACCCACATCAACGCCTACAACCTGGACCTCTACCTGCGGATCGCGCCGGAGCTGTACCTCAAGCGGCTCT
GCGTCGCCGGCATGGAGAAGGTGTTCGAGATCGGCCGCGTCTTCCGGAACGAGGGCGTCGATTTCAAGCACAACCCGGAG
TTCACCATCCTCGAGGCGTACGAGGCGCACAGCGACTACGAGAAGATGATGGTGCTGTGCCGGGAACTCATCCAGACCGC
GGCGGTCGCCGCGTACGGACGCGAGATCATCATGCGCCCCGGACCCGACGGCACGCTGGTGGAGGTCGACATCTCCGGCG
AATGGCCCGTCAAGACGATGCATCAGGCGGTCGCGGAAAAACTCGGCGTCGACGTCTCCCCCGAAACACCGCTCGCGGAA
CTGCAACGGCTGTGCGACGAACACGAGATCCCGTACCAGTCGACCTGGGACGCGGGCGCCGTCGCGCAGGAAATGTACGA
GCACCTCGTCGAGGACTACACCGAATTCCCCACGTTCTACACCAACTTCCCGACCTCGATGTCACCGCTGACGCGTCCGC
ACCCCACCATTCCGGGGGTCGCGGCGAAGTGGGACCTCGTCGCGTGGGGCGTCGAACTCGGCACCGCGTACAGCGAACTC
ACCGACCCCGTCGATCAGCGGAACCGGCTCACCGAGCAGTCGATGCTCGCCGCCGGCGGCGACGAGGAGGCGATGGAACT
CGACGAGGACTTCCTGCAGGCCCTCGAGCACGCCATGCCCCCGACCGGTGGGCTCGGCATGGGCGTCGACCGTGTCGTCA
TGCTCATCACCGGCGGCAGCATCCGCGAAACCCTCGCCTTCCCCCTGGCCAAGCCGAGGCAATGA

Protein sequence :
MSASTETHHASEAAVPTAPRPRPALGSKSGRLHQVPHIAGLILGVFSVLVFLWSISPVLRYYVHVPREYIDTYYFDAPDT
SLSWALVVALLAAALASRKRIAWWLLTIYLVLILITNVIVSITDRNVNAMVAAVVQVVLIGILVAARPEFYTRVRRGAGW
KALGVLIVGLAIGTLVGWGLVELFPGTLPQGERFLWALNRVTALAAADNEQFSGRPHGFVNTLLGLFGAMALLAAVITLF
RAQRSHNALTGNDESALRGLLLQYGADDSLGYFATRRDKAVVFAPSGKAAITYRVELGVCLASGDPIGDPEAWPHAIEAW
QTLASQYGWATAVMGASETGATAYNKAGLTVLQLGDEAILRTREFNLSGRDMRQVRQAVTRVRRQGVTVRIRRHRDVPPE
EMAEAIRLADAWRDTETERGFSMALGRLGDRLDGDCLLVEAIAEDGEIDGILSLVPWGPTGVSLDLMRRKPTSPNGVVEL
MVSELATTSDQFGITKVSLNFAVFRSVFEEGSRIGAGPILRIWRSILVFFSRWWQLEALYRSNVKYQPEWVPRFLCFDDN
RELLRVGFASAVAEGFVTLPRFGRSGTHDAIEHTGHHAAVPAALVAAEGLHSDGSAPGEGLAPTATGPKRPEQVRVRLDK
LTGLAEQGIDPYPVAYPPSHTVTEAVESPEGTTVRIAGRLLRIRDYGGVVFAVVRDWSGDIQVLVDEARVGTDRIRAFAA
EFDLGDLVEVAGVIGYSRRGALSLLANEWRMTGKCLHPLPDKWKGLSDPETRVRQRYVDLAINTDARRLLEARSAVVKSL
RDSLGGRGFLEVETPILQQVHGGANAAPFLTHINAYNLDLYLRIAPELYLKRLCVAGMEKVFEIGRVFRNEGVDFKHNPE
FTILEAYEAHSDYEKMMVLCRELIQTAAVAAYGREIIMRPGPDGTLVEVDISGEWPVKTMHQAVAEKLGVDVSPETPLAE
LQRLCDEHEIPYQSTWDAGAVAQEMYEHLVEDYTEFPTFYTNFPTSMSPLTRPHPTIPGVAAKWDLVAWGVELGTAYSEL
TDPVDQRNRLTEQSMLAAGGDEEAMELDEDFLQALEHAMPPTGGLGMGVDRVVMLITGGSIRETLAFPLAKPRQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lysX YP_003784331.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 3e-159 42
lysS1 YP_005686365.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 3e-159 42
lysS1 YP_005682181.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 1e-158 42
lysS1 YP_005684273.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 3e-159 42