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Name : lysS (ROP_35510) Accession : YP_002780743.1 Strain : Rhodococcus opacus B4 Genome accession: NC_012522 Putative virulence/resistance : Unknown Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 3850317 - 3853661 bp Length : 3345 bp Strand : - Note : catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA DNA sequence : ATGTCAGCTAGCACCGAGACACACCACGCGTCCGAGGCGGCCGTCCCCACCGCACCACGGCCCCGACCCGCCCTCGGCTC GAAGAGCGGGCGCCTGCACCAGGTTCCCCACATCGCGGGACTGATCCTGGGCGTCTTCTCGGTGCTCGTATTCCTGTGGA GCATCTCCCCGGTGCTGCGCTACTACGTGCACGTGCCGCGGGAGTACATCGACACGTACTACTTCGACGCCCCCGACACC AGCCTGTCGTGGGCGCTGGTAGTCGCGCTGCTCGCGGCGGCGCTGGCCAGCCGCAAACGCATCGCCTGGTGGCTGCTGAC GATCTATCTGGTGCTGATCCTGATCACCAACGTGATCGTGAGCATCACCGACCGGAACGTGAACGCGATGGTCGCCGCCG TGGTCCAGGTGGTGCTGATCGGCATCCTCGTCGCGGCCCGGCCCGAGTTCTACACCCGGGTCCGGCGCGGCGCCGGCTGG AAGGCGCTGGGCGTGCTCATCGTCGGGCTCGCGATCGGCACTCTCGTCGGGTGGGGACTCGTCGAATTGTTCCCCGGCAC CCTGCCGCAGGGCGAGCGATTCCTGTGGGCTCTCAACCGCGTGACCGCGCTGGCCGCCGCCGACAACGAACAGTTCAGCG GACGACCGCACGGATTCGTCAACACCCTCCTCGGCCTGTTCGGCGCCATGGCCCTGCTCGCCGCCGTCATCACCCTGTTC CGCGCCCAGCGCTCCCACAACGCGCTCACCGGCAACGACGAATCGGCGCTGCGCGGACTGCTGCTGCAGTACGGCGCGGA CGACTCCCTCGGCTACTTCGCCACCCGCCGCGACAAGGCCGTCGTGTTCGCGCCGAGCGGGAAGGCGGCCATCACCTACC GCGTCGAACTCGGCGTCTGCCTCGCCAGCGGTGATCCGATCGGCGACCCCGAGGCCTGGCCACACGCGATCGAGGCGTGG CAGACGCTCGCGTCCCAATACGGTTGGGCCACCGCCGTGATGGGCGCCAGCGAAACCGGGGCCACCGCCTACAACAAGGC CGGTCTCACCGTGCTCCAGCTCGGCGACGAGGCGATCCTCCGCACCCGCGAGTTCAACCTCAGCGGTCGCGACATGCGGC AGGTCCGCCAGGCCGTCACCCGCGTCCGCCGCCAGGGCGTCACCGTCCGGATCCGCAGGCACCGCGACGTCCCCCCCGAG GAAATGGCCGAGGCCATCCGGCTCGCGGACGCCTGGCGCGACACCGAGACCGAGCGCGGATTCTCGATGGCGCTCGGCCG GCTCGGCGACCGCCTCGACGGCGACTGCCTGCTGGTGGAGGCCATCGCCGAGGACGGTGAGATCGACGGCATCCTGTCGC TGGTTCCGTGGGGACCGACCGGTGTCTCGCTCGACCTGATGCGCCGCAAGCCGACCTCCCCCAACGGGGTCGTCGAACTG ATGGTGTCCGAACTGGCCACCACCTCCGACCAATTCGGCATCACCAAGGTGTCGCTGAACTTCGCGGTGTTCCGCTCGGT CTTCGAGGAGGGCTCCCGCATCGGGGCCGGACCCATTCTGCGAATCTGGCGCTCGATCCTCGTCTTCTTCTCCCGATGGT GGCAGCTCGAGGCGCTCTACCGATCCAACGTGAAGTACCAGCCGGAGTGGGTGCCGCGGTTCCTCTGCTTCGACGACAAC AGGGAACTGCTGCGCGTCGGATTCGCCTCGGCCGTCGCGGAAGGATTCGTGACGCTGCCGCGGTTCGGCCGTTCCGGGAC CCACGACGCCATCGAGCACACCGGCCACCACGCCGCGGTTCCCGCCGCACTCGTCGCCGCCGAGGGATTGCACTCCGACG GCAGCGCCCCCGGTGAGGGCCTCGCACCGACGGCGACCGGACCCAAACGTCCCGAGCAGGTCCGCGTCCGGCTCGACAAG TTGACCGGCCTGGCGGAACAGGGCATCGACCCCTATCCGGTCGCGTACCCGCCCAGCCACACCGTGACCGAGGCAGTGGA ATCCCCCGAGGGGACGACGGTCCGCATCGCGGGACGCCTGCTCCGGATCCGCGACTACGGCGGCGTCGTGTTCGCGGTGG TCCGGGACTGGTCCGGAGACATCCAGGTCCTCGTCGACGAAGCCCGGGTGGGAACCGATCGGATCCGGGCGTTCGCCGCC GAATTCGACCTCGGCGACCTCGTCGAGGTCGCGGGTGTCATCGGCTACAGCCGCCGGGGAGCGCTGTCGCTGCTGGCGAA CGAGTGGCGCATGACCGGCAAGTGCCTGCACCCCCTGCCCGACAAGTGGAAGGGACTGTCCGACCCGGAGACCCGGGTGC GGCAGCGGTACGTCGACCTCGCGATCAACACCGATGCCCGGCGCCTGCTCGAGGCCCGCAGCGCCGTCGTGAAGTCGTTG CGGGATTCGCTCGGCGGCCGCGGCTTCCTCGAGGTGGAGACCCCGATCCTGCAGCAGGTCCACGGCGGCGCGAACGCGGC CCCGTTCCTCACCCACATCAACGCCTACAACCTGGACCTCTACCTGCGGATCGCGCCGGAGCTGTACCTCAAGCGGCTCT GCGTCGCCGGCATGGAGAAGGTGTTCGAGATCGGCCGCGTCTTCCGGAACGAGGGCGTCGATTTCAAGCACAACCCGGAG TTCACCATCCTCGAGGCGTACGAGGCGCACAGCGACTACGAGAAGATGATGGTGCTGTGCCGGGAACTCATCCAGACCGC GGCGGTCGCCGCGTACGGACGCGAGATCATCATGCGCCCCGGACCCGACGGCACGCTGGTGGAGGTCGACATCTCCGGCG AATGGCCCGTCAAGACGATGCATCAGGCGGTCGCGGAAAAACTCGGCGTCGACGTCTCCCCCGAAACACCGCTCGCGGAA CTGCAACGGCTGTGCGACGAACACGAGATCCCGTACCAGTCGACCTGGGACGCGGGCGCCGTCGCGCAGGAAATGTACGA GCACCTCGTCGAGGACTACACCGAATTCCCCACGTTCTACACCAACTTCCCGACCTCGATGTCACCGCTGACGCGTCCGC ACCCCACCATTCCGGGGGTCGCGGCGAAGTGGGACCTCGTCGCGTGGGGCGTCGAACTCGGCACCGCGTACAGCGAACTC ACCGACCCCGTCGATCAGCGGAACCGGCTCACCGAGCAGTCGATGCTCGCCGCCGGCGGCGACGAGGAGGCGATGGAACT CGACGAGGACTTCCTGCAGGCCCTCGAGCACGCCATGCCCCCGACCGGTGGGCTCGGCATGGGCGTCGACCGTGTCGTCA TGCTCATCACCGGCGGCAGCATCCGCGAAACCCTCGCCTTCCCCCTGGCCAAGCCGAGGCAATGA Protein sequence : MSASTETHHASEAAVPTAPRPRPALGSKSGRLHQVPHIAGLILGVFSVLVFLWSISPVLRYYVHVPREYIDTYYFDAPDT SLSWALVVALLAAALASRKRIAWWLLTIYLVLILITNVIVSITDRNVNAMVAAVVQVVLIGILVAARPEFYTRVRRGAGW KALGVLIVGLAIGTLVGWGLVELFPGTLPQGERFLWALNRVTALAAADNEQFSGRPHGFVNTLLGLFGAMALLAAVITLF RAQRSHNALTGNDESALRGLLLQYGADDSLGYFATRRDKAVVFAPSGKAAITYRVELGVCLASGDPIGDPEAWPHAIEAW QTLASQYGWATAVMGASETGATAYNKAGLTVLQLGDEAILRTREFNLSGRDMRQVRQAVTRVRRQGVTVRIRRHRDVPPE EMAEAIRLADAWRDTETERGFSMALGRLGDRLDGDCLLVEAIAEDGEIDGILSLVPWGPTGVSLDLMRRKPTSPNGVVEL MVSELATTSDQFGITKVSLNFAVFRSVFEEGSRIGAGPILRIWRSILVFFSRWWQLEALYRSNVKYQPEWVPRFLCFDDN RELLRVGFASAVAEGFVTLPRFGRSGTHDAIEHTGHHAAVPAALVAAEGLHSDGSAPGEGLAPTATGPKRPEQVRVRLDK LTGLAEQGIDPYPVAYPPSHTVTEAVESPEGTTVRIAGRLLRIRDYGGVVFAVVRDWSGDIQVLVDEARVGTDRIRAFAA EFDLGDLVEVAGVIGYSRRGALSLLANEWRMTGKCLHPLPDKWKGLSDPETRVRQRYVDLAINTDARRLLEARSAVVKSL RDSLGGRGFLEVETPILQQVHGGANAAPFLTHINAYNLDLYLRIAPELYLKRLCVAGMEKVFEIGRVFRNEGVDFKHNPE FTILEAYEAHSDYEKMMVLCRELIQTAAVAAYGREIIMRPGPDGTLVEVDISGEWPVKTMHQAVAEKLGVDVSPETPLAE LQRLCDEHEIPYQSTWDAGAVAQEMYEHLVEDYTEFPTFYTNFPTSMSPLTRPHPTIPGVAAKWDLVAWGVELGTAYSEL TDPVDQRNRLTEQSMLAAGGDEEAMELDEDFLQALEHAMPPTGGLGMGVDRVVMLITGGSIRETLAFPLAKPRQ |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| lysS1 | YP_005682181.1 | lysyl-tRNA synthetase | Not tested | PiCp 7 | Protein | 1e-158 | 42 |
| lysS1 | YP_005684273.1 | lysyl-tRNA synthetase | Not tested | PiCp 7 | Protein | 3e-159 | 42 |
| lysX | YP_003784331.1 | lysyl-tRNA synthetase | Not tested | PiCp 7 | Protein | 3e-159 | 42 |
| lysS1 | YP_005686365.1 | lysyl-tRNA synthetase | Not tested | PiCp 7 | Protein | 3e-159 | 42 |
