Gene Information

Name : GAU_3221 (GAU_3221)
Accession : YP_002762733.1
Strain : Gemmatimonas aurantiaca T-27
Genome accession: NC_012489
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 2.7.7.58
Position : 3815716 - 3817392 bp
Length : 1677 bp
Strand : -
Note : -

DNA sequence : 
GTGCCTGGAGTGCCCACGCAGGAATGGCCGGTCGCCGATGCGACCCGGTATCGCGACGCGGGCTACTGGCAGGGCGAGTC
CATCGGCGCCTGGTTGCGCGCGCGCGCCGCGCAGCATGCTGAGCGCACCGCCGTTGTGGGCGGCAGCACCCGATGGAGCT
ACGCGCGTCTCGATCAGGAAGCGGACCGCGTGGCGGCGGGCTTTTTAGCGCGTGGTGTGCAACCGGGGGAACGGGTGGTG
GTGCAGTTGCCCAATGTCCCCGAGTTCCTGGCAGTGATGCTTGGGCTCATTCGGGCGGGTATCGTGCCCGTATTTGCCCT
GCCGGCCCATCGTCAGACCGAGATCGACAACATCGCCACGGTGTCCGGTGCGGTGGCGTATGTCATTCCGGCGGTACATG
AGAAGTTCGACTATCGCACGCTGGGGCGATCGCTGCGGGCTTCGCATGCGACCGTGCGCCACGTGGTCGTTGCCGGCGTC
GTGGAGCAGGACGATCCGGATCTCGAGTCGCTGGACGCGTTCCGTGGCGACGATCTGTCGTTGCTGATCGATCCGCCGTC
CTCGTCGGTGGCGTTCCTGCAATTGTCGGGTGGAAGCACAGGACTGTCGAAATTGATCCCTCGCACCCACGACGACTATC
TGTACAGCGTACGGGAGAGCGCGACGATCTGCGGTCTCTCACCGGCGTCGGTGTATCTGGTCGTGCTGCCGGTGGCGCAC
AATTTTCCGATGAGTTCACCCGGGTTTCTCGGCGCATTGGACGCTGGCGCCTGCATTGTGCTCAGTCCATCGCCGGCACC
CGACGTGGCCTTTGCATTGATCGAACAGGAACGGGTGACCATCGCTGCTGCCGTGCCACCCATCGCATTGATGTGGCTGG
AGGCCAAGGCGATCACATCGGCAGACCTGTCGAGCCTCGAAGTGTTGCAGGTGGGAGGGGCGAAGCTGACGCCGGCGATT
GCGGCACGGGTCACGCCGACGCTCGGCGCCACGTTGCAGCAGGTGTTCGGCATGGCAGAGGGATTGGTGAACTACACTCG
TCTCGACGACGATGTCGACACCATCTGCAACACACAGGGGCGCCCCATCAGTGCGGACGATGAACTGCGCATCGTGGACG
ATGATGATGTACCGGTCGCACCGGGTGCCATCGGTCACCTGCAGACCCGTGGCCCCTATACCATCCGCGGTTACTACCAG
AATGCCGCGGCCAATGCGCGCTCCTTCACCGATGACGGCTTCTATCGCACCGGTGATCTCGTGAGCCGTACGGCTGCCGG
GTATCTCGTGGTGCATGGCCGAGCCACCGATCAGATCAATCGGGGAGGCGAGAAGGTGTCTCCCGAGGAAGTGGAAGATC
ACCTCGTGGCGCACCCGGCCGTCCTCGATGCCGTGGTGGTATCGGTGCCCGACGACTATCTCGGCGAGCGATCCTGCGCG
TTCGTGATCCCACGCGCCGAACGCGTGCCCCCGCCGGTGCTCAAGTCGTGGATCCGCAATCGGGGGCTGGCCGCGTACAA
GGTGCCTGATCAAATCGTGTTCGTGGACGCGTTCCCGTCCACCGGTGTCGGCAAGACCAGTCGCAAGGATCTGCGTGGTG
CGCTGCGCGCACTCGCGGCAGAGCAGGCCACGGCCGCCGCAGCACCTGTTTCCACTCCTGATTCTCGCGTGTCATGA

Protein sequence : 
MPGVPTQEWPVADATRYRDAGYWQGESIGAWLRARAAQHAERTAVVGGSTRWSYARLDQEADRVAAGFLARGVQPGERVV
VQLPNVPEFLAVMLGLIRAGIVPVFALPAHRQTEIDNIATVSGAVAYVIPAVHEKFDYRTLGRSLRASHATVRHVVVAGV
VEQDDPDLESLDAFRGDDLSLLIDPPSSSVAFLQLSGGSTGLSKLIPRTHDDYLYSVRESATICGLSPASVYLVVLPVAH
NFPMSSPGFLGALDAGACIVLSPSPAPDVAFALIEQERVTIAAAVPPIALMWLEAKAITSADLSSLEVLQVGGAKLTPAI
AARVTPTLGATLQQVFGMAEGLVNYTRLDDDVDTICNTQGRPISADDELRIVDDDDVPVAPGAIGHLQTRGPYTIRGYYQ
NAAANARSFTDDGFYRTGDLVSRTAAGYLVVHGRATDQINRGGEKVSPEEVEDHLVAHPAVLDAVVVSVPDDYLGERSCA
FVIPRAERVPPPVLKSWIRNRGLAAYKVPDQIVFVDAFPSTGVGKTSRKDLRGALRALAAEQATAAAAPVSTPDSRVS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 4e-93 48
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 7e-93 48
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-93 48
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 6e-93 48
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-93 48
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-93 48
ybtE CAA21394.1 - Virulence HPI Protein 6e-93 48
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-91 47
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-91 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
GAU_3221 YP_002762733.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 2e-113 49
GAU_3221 YP_002762733.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 4e-95 45
GAU_3221 YP_002762733.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 7e-97 42