Gene Information

Name : entE (BMEA_B0017)
Accession : YP_002733855.1
Strain :
Genome accession: NC_012442
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 1.2.3.5
Position : 12098 - 13717 bp
Length : 1620 bp
Strand : -
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence : 
ATGACCATCGAATTTACCCATTGGCCACCGGCACGCGAACGCCTCTATCGCGAAAAGGGCTACTGGATCGACAAACCCCT
CACCCGCGCACTGGAAGAACAGGCCGCCATGCGGCCCGATGCGCCCGCTATCCTCTGCGGCGAGCGGCGTTTCACCTATG
CCGAACTCGACCGTCTGTCTTCCAACCTGGCCTCCCGGCTTGCGGCAGCCGGCATAGGCAAAGGCGACACGGCACTGGTG
CAACTGCCCAATATCGCCGAATTCTACATCGTGTTCTTCGCACTCATGAAAGCGGGCATCGCGCCCGTCAATGCGCTTTT
CAGCCATCGCAAGCTGGAGCTTGGCGCCTATGCCAGCCAGATCGAACCACGGCTGCTGATCGGCTCACGCCAGCATGAAC
TCTTCATGGATGACGCCTTTGCCCGCGACCTTGGCAAAAATCTTTCCGCACCGCTGCTAACACTTTTTGCAGGCGAGGCC
GACCCTGCGTCGAGCCTCGACCACTGGATCGCCACGCCTGCGGACAAAGCCGTTCCCTTCTCGCCAACCGGCGCTGGCGA
AGTCGCCTTTTTCCAGCTTTCCGGCGGCAGCACCGGCACGCCGAAACTCATCCCGCGCACGCATAATGATTATGATTACA
GCGTGCGCGCCAGCGCGGAAATCTGCGCGCTGACACCGCAGACCCGTTTTCTCTGCGCCCTGCCCACCGCGCATAATTAT
CCAATGAGTTCGCCCGGCGCGCTCGGCGTTTTCCATGCGGGCGGCTGTGTCGTCATGGCCCCCAACCCGGAACCGCTCAA
CTGCTTCTCCATCATCGAACGTCATGGCGTAAACATGGTGGCACTGGTGCCTCCGGCAGTGGCGCTCTGGCTACAGGCAG
CCCCCGATCATCTGGCAGCACTCAGCTCGCTGAAACTCGTGCAGGTGGGTGGCGCAAGCTTTGCGGAGGCGCTTGCCCGG
CAGGTGCCACAGGTTCTGGGCTGCAAGCTGCAACAGGTCTTCGGCATGGCCGAGGGGCTGGTCAACTATACCCGCCTTGA
CGACCCGGACGAGATCGTCTTCACCACCCAGGGCCGTCCGATCAGCCCGGACGATGAAATCAGGATCGTCGATGAGGATG
GCGAACCCGTGGCCGAAGGCCAGCCAGGAATGCTGGCCACGCGCGGGCCTTACACGTTCTGCGGCTATTATCGCAGCCCC
GAACATAATGCGCAGGTCTTCGACAGTGAAGGCTTTTATTATTCGGGCGACGTGGTCCAGCGCACGCCGGAAGGCTATCT
GCGCGTCGTGGGCCGCGTGAAGGACCAGATTAATCGCGGCGGCGAGAAAGTTGCCGCCGAGGAGATTGAAAACCTAATCC
TCCTCCATCCCGATGTGACGCACGCCGCACTTGTCGCCATGCAGGATGAATTGCTGGGCGAGAAAAGCTGCGCTTTCATC
GTCTCGCGCAATCCCGATCTCAAGCCGCCTGTTCTGCGGCGTCACCTGCTGGCGCTGGGCGTTGCCGAATACAAGCTTCC
AGACCGCATCAGGCTCATTGAAACCATGCCGCTCACCGCAGTTGGCAAGATCGACAAAAAGCATCTGCGCAAGCTTTCAA
GCCCATCCTCCCCCGGTTAG

Protein sequence : 
MTIEFTHWPPARERLYREKGYWIDKPLTRALEEQAAMRPDAPAILCGERRFTYAELDRLSSNLASRLAAAGIGKGDTALV
QLPNIAEFYIVFFALMKAGIAPVNALFSHRKLELGAYASQIEPRLLIGSRQHELFMDDAFARDLGKNLSAPLLTLFAGEA
DPASSLDHWIATPADKAVPFSPTGAGEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICALTPQTRFLCALPTAHNY
PMSSPGALGVFHAGGCVVMAPNPEPLNCFSIIERHGVNMVALVPPAVALWLQAAPDHLAALSSLKLVQVGGASFAEALAR
QVPQVLGCKLQQVFGMAEGLVNYTRLDDPDEIVFTTQGRPISPDDEIRIVDEDGEPVAEGQPGMLATRGPYTFCGYYRSP
EHNAQVFDSEGFYYSGDVVQRTPEGYLRVVGRVKDQINRGGEKVAAEEIENLILLHPDVTHAALVAMQDELLGEKSCAFI
VSRNPDLKPPVLRRHLLALGVAEYKLPDRIRLIETMPLTAVGKIDKKHLRKLSSPSSPG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-86 44
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-86 44
ybtE CAA21394.1 - Virulence HPI Protein 2e-86 44
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-86 44
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 2e-86 44
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 1e-86 44
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-86 44
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 2e-86 44
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 2e-86 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_002733855.1 enterobactin synthase subunit E VFG0932 Protein 1e-143 58
entE YP_002733855.1 enterobactin synthase subunit E VFG0168 Protein 1e-107 47
entE YP_002733855.1 enterobactin synthase subunit E VFG1817 Protein 4e-88 41