Gene Information

Name : lysS (EpC_29250)
Accession : YP_002649906.1
Strain : Erwinia pyrifoliae Ep1/96
Genome accession: NC_012214
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 3224422 - 3225942 bp
Length : 1521 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCTGAACAACAACCGCAGGCCGCTGACGCCGCACTTGAGCTGAACAACGAACTGAAAGCACGGCGCGAAAAGCTGGT
GGCACTGCGCGAAACCGGCGTGGCGTTCCCGAACGATTTCCGCCGTGACCGCACATCTGACCAGCTGCACGCCGAATTTG
ACGGCAAAGAGAACGATGAGCTGGAAGCGCTGGGCATTGAGGTCAGCGTTGCGGGCCGTATGATGACGCGTCGTATCATG
GGCAAAGCCTCTTTCGTCACCCTGCAGGATGTCGGTGGCCGTATTCAGCTGTACGTGTCCCGGGACGATCTGGCGGATGG
CATCTACAACGAACAGTTCAAAAAATGGGATCTCGGCGATATCCTCGGCGCGCGCGGCAAGTTGTTCAAGACCAAAACTG
GCGAGCTGTCGATCCACTGCAGCGAACTGCGTCTGCTGACCAAAGCTCTTCGCCCGTTACCGGACAAGTTCCACGGCCTG
GCCGATCAGGAAACCCGTTATCGTCAGCGTTACCTTGATCTGATCGCCAACGACGATTCACGTAAAACCTTTAAGATCCG
CTCACAGATCATGGCCGGTATCCGCAACTTTATGGTCGGCCGTGACTTTATGGAAGTGGAAACCCCGATGATGCAGGCGA
TCCCTGGCGGCGCTTCTGCCCGGCCGTTTATCACTCACCACAATGCGCTGGATCTTGATATGTATCTGCGTATTGCCCCG
GAGCTGTATCTGAAGCGTCTGGTGGTGGGTGGCTTCGACCGCGTGTTTGAAATCAACCGTAACTTCCGTAACGAAGGGAT
TTCGCCGCGCCATAACCCAGAGTTCACCATGATGGAACTCTATATGGCGTATGCCGACTACAAAGACCTGATTGAACTGA
CCGAAAGCCTGTTCCGTACGCTGGCGCAGGACGTGCTGGGCAGCACCGTGGTGCCTTACGGCGACCAGCAGTTCGACTTC
GGTAAGCCTTTCGCACAACTGACGATGAAAGAAGCGATACTGAAATATCGTCCGCAGACCGATCTGGCCGATCTTGCTGA
CTTCGAAAAATCAGTCGCCATCGCCCAGTCTCTGGGGATTAACGTTGAGAAGAGCTGGGGGCTGGGCCGTCTGGTGACCG
AGATCTTCGAAGAAACGGCGGAAGCGCATCTGATCCAGCCGACCTTTATTACCGAATACCCGGCTGAAGTCTCCCCGCTG
GCGCGTCGTAACGATGTCGATCCGGAAATTACCGATCGTTTTGAGTTCTTTATCGGCGGCCGTGAAATCGGCAACGGCTT
CTCGGAGCTGAACGATGCACAAGACCAGGCAGAACGTTTCCTGCAGCAGGTGAATGCCAAAGACGCGGGTGACGACGAAG
CGATGTTCTACGACGAAGATTACGTTACCGCGCTGGAGCACGGCCTGCCGCCAACCGCCGGTCTGGGCGTTGGCATTGAC
CGTATGGTCATGCTGTTTACCAACAGCCATACCATTCGCGACGTGATCCTGTTCCCGGCGCTGCGTCCAGGCAGCAAGTA
A

Protein sequence :
MSEQQPQAADAALELNNELKARREKLVALRETGVAFPNDFRRDRTSDQLHAEFDGKENDELEALGIEVSVAGRMMTRRIM
GKASFVTLQDVGGRIQLYVSRDDLADGIYNEQFKKWDLGDILGARGKLFKTKTGELSIHCSELRLLTKALRPLPDKFHGL
ADQETRYRQRYLDLIANDDSRKTFKIRSQIMAGIRNFMVGRDFMEVETPMMQAIPGGASARPFITHHNALDLDMYLRIAP
ELYLKRLVVGGFDRVFEINRNFRNEGISPRHNPEFTMMELYMAYADYKDLIELTESLFRTLAQDVLGSTVVPYGDQQFDF
GKPFAQLTMKEAILKYRPQTDLADLADFEKSVAIAQSLGINVEKSWGLGRLVTEIFEETAEAHLIQPTFITEYPAEVSPL
ARRNDVDPEITDRFEFFIGGREIGNGFSELNDAQDQAERFLQQVNAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGVGID
RMVMLFTNSHTIRDVILFPALRPGSK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 5e-169 69

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_002649906.1 lysyl-tRNA synthetase VFG1668 Protein 3e-169 69