Gene Information

Name : lysS (Sca_0169)
Accession : YP_002633267.1
Strain : Staphylococcus carnosus TM300
Genome accession: NC_012121
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 171510 - 173003 bp
Length : 1494 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCAGAAGAAATGAATGACCAAATGCAGGTCCGCCGTCAAAAATTACAAGAATTAATCGATTTAGGAATTGATCCATT
CGGTAAACGTTTTGATCGTACAGCAAGTGCTGAAGAATTAAAAGAAGAATGGGATCAATATTCTAAAGAAGAGTTGCACG
AAAAAGAAGATGAAGAAGCAGCGCATGTAGCAATTGCAGGCCGTTTAATGACTAAACGCGGTAAAGGTAAAGCTGGATTT
GCGCATATTCAAGATTTATCTGGACAAATTCAAATTTATGTACGTAAAGACCAAGTCGGCGAAGAACAATTTGCAATTTG
GAAAATGGCTGACTTAGGAGACATCGTAGGTATTGAAGGCGTTATGTTTAAAACAAACACAGGTGAGTTAAGTGTCAAAG
CTAAATCATTTACTTTGTTATCAAAATCATTACGTCCATTACCAGACAAATTCCATGGTTTGCAAGATGTTGAACAACGT
TATCGTCAACGTTATTTAGATTTAATTACAAATCAAGAAAGTACACAAACATTCATTAACCGCAGTAAAATCATTCAAGA
AATGCGTAACTATTTAAATAGTAAAGGTTTCTTAGAAGTTGAAACACCTATGATGCACCAAATCGCAGGTGGTGCTGCTG
CACGCCCATTCGTAACACATCACAATGCATTAGATGCAACGTTATATATGCGTATTGCGATTGAACTACACTTGAAACGT
TTGATTGTAGGCGGATTAGAAAAAGTATATGAAATTGGACGTGTATTCCGTAACGAAGGTGTATCAACACGCCATAACCC
TGAATTTACTATGATCGAATTATATGAAGCTTATGCTGATTTCCATGACATCATGGATTTAACAGAAGGCACAATTCGCC
ATATTTCTAAAAAAGTACTAGGCACTGCAGTAGTTCCTTATGGTGAATACGAAGTAGATTTAGATTCACCTTGGAAACGA
GTTCATATTGTAGATGCTGTTAAAGAAGCAACTGGTGTAAACTTCTTTGATGTTAAATCTGATGAAGAAGCACGTGAACT
TGCTAAGGAACATGGTATTGAAATTACAGATCATATGACATATGGTCATATTTTGAATGAATTCTTTGAACAAAAAGTAG
AAGAAACACTTATTCAACCTACATTTGTATATGGACATCCAATTGAAATTTCACCACTTGCTAAGAAAAATCCGGAAGAT
CCGAGATTTACAGATCGTTTCGAATTGTTTATTGTGGGTCGTGAACATGCGAATGCATTTACAGAATTAAATGATCCTAT
TGATCAACGCGAAAGATTCGAAGCGCAATTGAAAGAAAAAGAACAAGGCAACGATGAAGCGCATGAAATGGATGAAGATT
ATATTGAAGCATTAGAATACGGTATGCCTCCTACAGGCGGTCTAGGAATTGGTATTGATAGACTTGTAATGCTATTAACT
AATTCTCCATCTATCAGAGATGTATTGTTATTCCCATACATGCGCCAAAAATAA

Protein sequence :
MSEEMNDQMQVRRQKLQELIDLGIDPFGKRFDRTASAEELKEEWDQYSKEELHEKEDEEAAHVAIAGRLMTKRGKGKAGF
AHIQDLSGQIQIYVRKDQVGEEQFAIWKMADLGDIVGIEGVMFKTNTGELSVKAKSFTLLSKSLRPLPDKFHGLQDVEQR
YRQRYLDLITNQESTQTFINRSKIIQEMRNYLNSKGFLEVETPMMHQIAGGAAARPFVTHHNALDATLYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADFHDIMDLTEGTIRHISKKVLGTAVVPYGEYEVDLDSPWKR
VHIVDAVKEATGVNFFDVKSDEEARELAKEHGIEITDHMTYGHILNEFFEQKVEETLIQPTFVYGHPIEISPLAKKNPED
PRFTDRFELFIVGREHANAFTELNDPIDQRERFEAQLKEKEQGNDEAHEMDEDYIEALEYGMPPTGGLGIGIDRLVMLLT
NSPSIRDVLLFPYMRQK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 5e-117 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_002633267.1 lysyl-tRNA synthetase VFG1668 Protein 3e-117 51