Gene Information

Name : orfX (MCCL_0026)
Accession : YP_002559429.1
Strain : Macrococcus caseolyticus JCSC5402
Genome accession: NC_011999
Putative virulence/resistance : Unknown
Product : rRNA large subunit methyltransferase
Function : -
COG functional category : S : Function unknown
COG ID : COG1576
EC number : -
Position : 31891 - 32370 bp
Length : 480 bp
Strand : +
Note : SPOUT methyltransferase family protein; crystal structure shows homodimer; in Escherichia coli this protein methylates pseudouridine at position 1915 of the 23S ribosomal RNA

DNA sequence :
ATGAAAATAACAATAATAACTGTGGGTAAGTTGAAAGAGAAGTACTGGAAGATGGCTGTAGACGAGTATGTAAAAAGACT
TGGTGCATACTCGAAAATAGAACTCGTTGAGGTAGCTGACGAGAAAGATTCGGATAATATGAGTGAGAAAGATATCGAGA
TTGCGAAGCGGAAAGAAGCGGAACGAATATTGAGTAAGGTGAAAGATGATAGCTTTGTCTACACGTTAGAGATTCTTGGC
AAGCAGCTCGACTCTGTGCAGTTAGCAAAAGAGATGGAGCAAAAGATGAATACCGGTAAAAGTCATCTGACGTTTATTAT
CGGCGGATCAAATGGCCTTCATCAAAATGTTATGGATCGCTCTAACTACGCCCTATCATTTAGCAAGATGACATTTCCGC
ATCAGATGATGAAAGTGATACTGCTGGAGCAGATTTATCGAGGATTTAGGATAATGAAGGGACAGGCCTACCATAAATAA

Protein sequence :
MKITIITVGKLKEKYWKMAVDEYVKRLGAYSKIELVEVADEKDSDNMSEKDIEIAKRKEAERILSKVKDDSFVYTLEILG
KQLDSVQLAKEMEQKMNTGKSHLTFIIGGSNGLHQNVMDRSNYALSFSKMTFPHQMMKVILLEQIYRGFRIMKGQAYHK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SE0023 NP_763578.1 rRNA large subunit methyltransferase Not tested SCCpbp4 Protein 3e-53 68
SERP2529 YP_190070.1 rRNA large subunit methyltransferase Not tested Type-II SCCmec Protein 3e-53 68
SSP0026 YP_300116.1 rRNA large subunit methyltransferase Not tested SCC15305RM Protein 9e-53 68
orfXhom BAB83497.1 open reading frame X Not tested SCC 12263 Protein 1e-49 66
orfX YP_251938.1 rRNA large subunit methyltransferase Not tested SCCmec Protein 2e-52 66
unnamed BAC53844.1 open reading frame X Not tested SRImec-III and SCCmec-III region Protein 5e-53 65
SAV0024 NP_370548.1 rRNA large subunit methyltransferase Not tested Type-II SCCmec Protein 8e-53 65
unnamed BAC57492.1 hypothetical protein Not tested Type-IIIinv SCCmec Protein 5e-53 65
unnamed BAB72121.1 hypothetical protein Not tested Type-IVa SCCmec Protein 5e-53 65
unnamed BAB72138.1 open reading frame X Not tested Type-IVb SCCmec Protein 5e-53 65
unnamed BAC67575.1 open reading frame X Not tested Type-IVc SCCmec Protein 5e-53 65
unnamed BAA82243.1 orfX Not tested Type-II SCCmec Protein 5e-53 65
orfX AAL26648.1 unknown Not tested SCCcap1 Protein 7e-52 65
unnamed BAD24821.1 conserved hypothetical protein orfX Not tested Type-V SCCmec Protein 5e-53 65
orfX BAA86653.1 open reading frame X Not tested Type-I SCCmec Protein 5e-53 65
unnamed BAG06184.1 conserved hypothetical protein orfX Not tested Type-VII SCCmec Protein 5e-53 65
unnamed BAB47681.1 open reading frame X Not tested Type-III SCCmec Protein 5e-53 65
SAR0024 YP_039501.1 rRNA large subunit methyltransferase Not tested Type-II SCCmec Protein 8e-53 65
unnamed ACL99851.1 hypothetical protein Not tested Type-V SCCmec Protein 1e-34 64