Gene Information

Name : lysS (MLBr_01393)
Accession : YP_002503621.1
Strain : Mycobacterium leprae Br4923
Genome accession: NC_011896
Putative virulence/resistance : Unknown
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 1671856 - 1674975 bp
Length : 3120 bp
Strand : -
Note : catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA

DNA sequence :
GTGCTGGCGCTGTTAGCCGCCGCGCTAACCACGCGCAAACGCATCGCCTGGCTGCTGTTGCTCGGCAATATGGTGCTCGC
CGCCGTGTTAAACGCTGTGGATATGGCAGCGAATGGCAACACCCCAGCCGAGACATTCGGCGAAAACCTCGGGTTCGCAG
TGCATGTTGTTGCGATCTTGCTGCTGGTTTTGAGCTATCGGGAATTCTGGGCCAAAGTCCGCCGAGGCGCGCTGTTCAAA
GCGGCTGCGGTGCTGGTTGCCGGAGACGTGATCGGGATACTGTTGTCTTTGGGCCTGGTCGAGCTGTTCCCCGGATCGCT
AGCGCGGCAGGATCGCCTGCCGTATGTGGCCAATCGGGTGGTCGGATTCGCACTTGCCGATCCCGACCTCTTCAGCGGCA
AACCGCACGTCTTACTCAACGCAATCTTCGGCTTGTTCGGCGCCCTCGCGTTGATCATGGCCACTATCGTGCTGTTTCAG
TCCCAGCGTGCAGATAACGCCCTGACCGGCGAGGATGAGTCCGCCATCCGCGGGCTACTTGAGCTGTATGGCAAGAACGA
CTCACTGGGTTACTTCGCCACCCGGCGCGATAAGTCGGTGATCTTCGCGCACAATGGCCGCGCGGCCATAACCTATCGAG
TTGAGATAGGAGTCTGCCTAGCCAGCGGCGACCCAGTAGGCGATCCCGGGGCATGGCCGCAGGCCGTCGATGCGTGGTTA
GAGCTTTGCCAGACGTACGGTTGGGCGCCCGGTGTGATGGGGGCCAGTTCACAGGGGGCCCAGGTATTTCGTAGGGCCGG
CTTCAACGCCATAGAACTCGGCGACGAGGCGATCTTGCGGACTGCCGTTTACAAACTTTCCGGCCCCGACATGCGCGGAG
TGCGCCAGGCGGTGACACGGGCACGCCGGGCCGGACTGACCGTACGCATTAGACGGCACAGCGACATCTCCGCCAACGAG
ATGGCAGACACCATCGCGCGTGCTGACGCCTGGCGCGACACCGAATTCGAACGTGGCTTTTCGATGGCGCTGGGCCGGCT
CGGCGATCCGGCTGATTCAGATTGCCTGCTGGTCGAAGCAGTGGACCGCGACGACCATGTCGTTGCGATACTGTCACTGG
TCCCGTGGGGAACCACCGGCGTCTCGTTGGACGTGATGCGCCGTTCTCCACAATCACCCAACGGCACCATCGAACTCATG
GTCAGCGAGCTAGCCCTCAAGGGCGAGACTCTTGGGATCGCCCGCATTTCGTTGAATTTTACGATGTTTCGCGCCGCGTT
CGAGCAAGGTGCTCAACTCGGCGCTGGCCCAATTGCACGGTTGTGGCGGGGGCTGCTGTTGTTTTTCTCACGGTGGTGGC
AGTTGGAGACGCTGTACCGCTCGAACATAAAATACCTTCCTGACTGGGTCCCTCGTTACGCCTGCTACGAGGACGCCCGG
TTGATTCCGCGGGTGGGGGTTGCCTCGGTGATCGCCGAGGGTTTCCTTGTGTTACCGTTCAGCCGACGCGGCCGGGTGCA
CACCGGCCATCATCCGGCTGTGCCAGCGCGGTTAGCAGAATCCGGGCTGCTGCACCACGATGGGTCGACTCCAGATGTCA
GCGGATTGCAAACGGCGGACGTCGATTTGGAAGTAGCGAATTCCCGTATACCCGAACAGGTACGGGTGCGTCTGGCAAAA
CTTAAGACATTGCAACTCAACGGTATTGACGCTTATCCGGTGGGACACCCGCCTAGCCACACAGTCGCCCAGGCTCTGGA
TGCCGACGACGAAGGCACCGTCTCGATATCTGGACGCATATTGCGGATACGTGACTATGGCGGCGTGTTGTTTGCTCATG
TGCGTGACTGGTCGGGCGAAATACAAGTGCTGCTGGATAATTTAGTGCTGGAGTGCTGCTGCATCGCAGATTTCACCGCA
GCCATTGATCTGGGCGATATAGTCGAAATGACCGGGAACATGGGCTTCAGCAAAAACGGGACTCGCTCGTTGATAGTACG
CAACTGGCGGCTGATCGGTAAATGCTTACGGCCGCTACCGAACAAATGGAAGGGGTTAACCGACCCAGAAGCCCGGGTGC
GAACCCGTTATGTCGATCTGGCAGTCAATACCGAGTCCCGTAACCTGATCATGGCTCGCAGCTGCGTGTTGCGCTCGGTC
AGGGAGATGCTGTTCGCTAAAGGATTTGTCGAAGTCGAGACCCCAATCCTGCAACAAATTCACGGCGGCGCCACTGCCCG
GCCATTCGCCACTCGCATCAATACCTATGACATGGATCTGTTCCTACGCATTGCCCCGGAGCTCTATCTTAAGAGGCTGT
GCATCGGGGGTGTAGAGCGGGTCTTCGAGCTGGGCCGGGCCTTCCGTAACGAGGGAGTAGACTTTAGTCACAATCCGGAG
TTCACCTTGCTGGAGGCCTACCAGGCGCACGCAGACTACCTAATGTGGATCGACGGCTGCCGAGAATTGATCCAGAATGC
CGCCGAAGCCGCGAACGGTACACAGACCCTAATGCGGCCGCGGATCGAGGGAGCAAGCGGAACCGCAAACCACCTCGAGC
CGATCGACATCTCCGGCGTCTGGCCGGTGAAGACCGTGTACGAAGCAGTATCCGAAGCGCTCGGAGAATGTGTCGACACC
AGCACTGAGTTGGCCACTCTGCGTAAATTGTCCGACGCCGCACACATACCTTATTGGCCACACTGGGATACTGGTGCGGT
GGTTCTGAAGCTCTATGAGCACCTGGTTGAGAACCGAACCGATCAACCGACATTCTACATCGACTTTCCAACGTCCGTGT
CACCGTTAACCCGGCCACATAGAAGTAAGCCCGGTGTCGCCGAGCGATGGGACCTGGTGGCGTGGGGTATAGAATTAGGT
ACCGCCTACAGCGAACTAACCGACCCGGTAGAGCAGCGGCGTCGTCTGCACGAACAGTCCTTATTGGCCGTCGGCGGAAA
CCCCGAAGCGATGGAGCTCGACGAAGACTTCCTACAAGCCATGGAATACGCTATGCCACCGACCGGCGGCCTGGGAATGG
GCATAGACCGTCTCGTGATGTTAATCACCGGCCGCAGCATTCGCGAAACGCTACCGTTCCCACTGGCCAAGCCGCACTAG

Protein sequence :
MLALLAAALTTRKRIAWLLLLGNMVLAAVLNAVDMAANGNTPAETFGENLGFAVHVVAILLLVLSYREFWAKVRRGALFK
AAAVLVAGDVIGILLSLGLVELFPGSLARQDRLPYVANRVVGFALADPDLFSGKPHVLLNAIFGLFGALALIMATIVLFQ
SQRADNALTGEDESAIRGLLELYGKNDSLGYFATRRDKSVIFAHNGRAAITYRVEIGVCLASGDPVGDPGAWPQAVDAWL
ELCQTYGWAPGVMGASSQGAQVFRRAGFNAIELGDEAILRTAVYKLSGPDMRGVRQAVTRARRAGLTVRIRRHSDISANE
MADTIARADAWRDTEFERGFSMALGRLGDPADSDCLLVEAVDRDDHVVAILSLVPWGTTGVSLDVMRRSPQSPNGTIELM
VSELALKGETLGIARISLNFTMFRAAFEQGAQLGAGPIARLWRGLLLFFSRWWQLETLYRSNIKYLPDWVPRYACYEDAR
LIPRVGVASVIAEGFLVLPFSRRGRVHTGHHPAVPARLAESGLLHHDGSTPDVSGLQTADVDLEVANSRIPEQVRVRLAK
LKTLQLNGIDAYPVGHPPSHTVAQALDADDEGTVSISGRILRIRDYGGVLFAHVRDWSGEIQVLLDNLVLECCCIADFTA
AIDLGDIVEMTGNMGFSKNGTRSLIVRNWRLIGKCLRPLPNKWKGLTDPEARVRTRYVDLAVNTESRNLIMARSCVLRSV
REMLFAKGFVEVETPILQQIHGGATARPFATRINTYDMDLFLRIAPELYLKRLCIGGVERVFELGRAFRNEGVDFSHNPE
FTLLEAYQAHADYLMWIDGCRELIQNAAEAANGTQTLMRPRIEGASGTANHLEPIDISGVWPVKTVYEAVSEALGECVDT
STELATLRKLSDAAHIPYWPHWDTGAVVLKLYEHLVENRTDQPTFYIDFPTSVSPLTRPHRSKPGVAERWDLVAWGIELG
TAYSELTDPVEQRRRLHEQSLLAVGGNPEAMELDEDFLQAMEYAMPPTGGLGMGIDRLVMLITGRSIRETLPFPLAKPH

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lysS1 YP_005682181.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 3e-162 43
lysS1 YP_005684273.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 8e-163 43
lysX YP_003784331.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 8e-163 43
lysS1 YP_005686365.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 8e-163 43