Gene Information

Name : Mnod_6322 (Mnod_6322)
Accession : YP_002501420.1
Strain : Methylobacterium nodulans ORS 2060
Genome accession: NC_011894
Putative virulence/resistance : Unknown
Product : transposase IS66
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG3436
EC number : -
Position : 6419867 - 6421384 bp
Length : 1518 bp
Strand : +
Note : PFAM: transposase IS66; KEGG: xau:Xaut_4598 transposase IS66

DNA sequence :
GTGGCTGCGCCTGCTTCACCCTCGCCCGACGATCCCGAGACGCTCAAGGCGCTGCTCGCCGAGGAGCGGGCCGAGAACGA
GCGGCTGCGCCAGATCATCAAGGCGATGCAGCGTCATCGCTTCGGCCGTCGCGCCGAGAGCCTGCCCGAGGACCAGCTCC
TGCTCGGGCTGGAGGAGGCCGAGCAGGTCGAAGCCGAGGGTCTTGCGGCCGAGGAAACCGCGCCGGCGGTGAGGGCCGAG
CGTACAGCGCGCCGGCGCAACAATCGCGGGGCCCTGCCCGCCCATCTGCCGCGCGTCGAGCGCGTGGTCGACATCGAGAG
CCCCGTCTGCCCCTGCTGCTCGGGCAGCCTGCACCGGATCGGCGAGGACGTGTCCGAGCGCCTCGACATCGTACCGGCCC
AAGTCCGGGTCATTGTGATCCGGCGCCCGCGTTACGCGTGCCGTGCCTGCGAGGCTATTGTGCTGCAGGCGTCCGCGCCG
GCGCGGCTGATCGAGGGCGGGTTGCCCACCGACGACCTCGTCGCGCACGTGCTCGTCGCCAAGTACGCCGACCACCTGCC
ACTCTATCGTCAGGCACAGATCTTCGCGCGTCAGGGCATAGCTCTCGACCGCTCGACGCTCGCGGATTGGGTCGGCCGTG
CTGCGTTCCTGCTGCGGCCGGTCCACGCGCGGCTGCTGGGGAGGCTGAAGGCTTCGGGCAAGCTGTTTGCCGACGAGACC
AGCGCGCCGGTGCTCGATCCCGGACGCGGCCGCACCAAGACAGGCCAGCTCTGGGCGTATGCCCGCGACGACCGGCCCTG
GGGCGGCACCGATCCGCCGGGCGTTGCCTACGTCTACGCACCCGACCGCACGGCCAAGCAGCCCATCGCGCATCTTGCCG
GCTTCCGGGGCGTGCTGCAGGTCGATGGCTATGACGGCTACAAGGCGCTGGCCAGGCAAGGCGAGGTCCGGCTCGCCTAC
TGCTGGAGCCATGTACGGCGGGCGTTCTACGATCTTGATGGGCCGATCGCGGCCGAGGTGCTCACGCGCATCGCGGGCCT
TTACCGGATCGAGGCCGAGATCCGCGGCCGTCCTGCCGAGGAGCGCCGCACCGCACGTCAGGCGCGCAGCCGTCCCATCC
TGGAGGCCCTAGAGCCCTGGCTGCGGGAGAAGCTCGCCCTCATCAGCCAGAAGAGCAAGCTGGCCGAAGCGATCCGCTAC
GCGCTGAGCCGCTGGGCTGGCCTGAGCCTGTTCCTCGATGACGGGCGGGTCGAACTCGACACCAACGTGGTCGAGCGCGC
GATCCGTCCCCTGGCCCTGACGAGAAAGAATGCTCTCTTCGCCGGCTCGGATGGCGGGGCCGCGCACTGGGCGGTGATCG
CCTCGCTGATCGAGACCTGCAAGCTCACGGGCGTCGGACCACAGGCCTATCTTGCCGACGTGATCACCCGCATCATCGAG
GGCCATCCCAACAGCCGCATCGACGAGCTGCTGCCCTGGGCCTACCCGGTCACGCCCACGCTCAGGGATGTGGCCTGA

Protein sequence :
MAAPASPSPDDPETLKALLAEERAENERLRQIIKAMQRHRFGRRAESLPEDQLLLGLEEAEQVEAEGLAAEETAPAVRAE
RTARRRNNRGALPAHLPRVERVVDIESPVCPCCSGSLHRIGEDVSERLDIVPAQVRVIVIRRPRYACRACEAIVLQASAP
ARLIEGGLPTDDLVAHVLVAKYADHLPLYRQAQIFARQGIALDRSTLADWVGRAAFLLRPVHARLLGRLKASGKLFADET
SAPVLDPGRGRTKTGQLWAYARDDRPWGGTDPPGVAYVYAPDRTAKQPIAHLAGFRGVLQVDGYDGYKALARQGEVRLAY
CWSHVRRAFYDLDGPIAAEVLTRIAGLYRIEAEIRGRPAEERRTARQARSRPILEALEPWLREKLALISQKSKLAEAIRY
ALSRWAGLSLFLDDGRVELDTNVVERAIRPLALTRKNALFAGSDGGAAHWAVIASLIETCKLTGVGPQAYLADVITRIIE
GHPNSRIDELLPWAYPVTPTLRDVA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
c3563 NP_755438.1 hypothetical protein Not tested PAI I CFT073 Protein 2e-80 48
st57 CAC81895.1 ST57 protein Not tested LEE II Protein 2e-90 47
tnp AEA34686.1 transposase Not tested Not named Protein 2e-90 47
Z1131 NP_286666.1 hypothetical protein Not tested TAI Protein 8e-90 47
unnamed ACU09439.1 IS66 family element transposase Not tested LEE Protein 2e-90 47
Z1570 NP_287074.1 hypothetical protein Not tested TAI Protein 8e-90 47
unnamed CAC39285.1 hypothetical protein Not tested LPA Protein 1e-90 47
ECs4547 NP_312574.1 hypothetical protein Not tested LEE Protein 3e-90 47
unnamed AAC31494.1 L0015 Not tested LEE Protein 1e-90 47
Z4337 NP_289562.1 hypothetical protein Not tested OI-122 Protein 1e-90 47
Z5098 NP_290249.1 prophage-associated protein Not tested LEE Protein 1e-90 47
unnamed AAL57570.1 unknown Not tested LEE Protein 9e-91 47
unnamed CAI43806.1 hypothetical protein Not tested LEE Protein 7e-91 47
BCAM0248 YP_002232880.1 putative transposase Not tested BcenGI11 Protein 1e-89 45
Z4340 NP_289564.1 hypothetical protein Not tested OI-122 Protein 8e-84 43
Z1161 NP_286696.1 hypothetical protein Not tested TAI Protein 5e-85 43
Z1600 NP_287104.1 hypothetical protein Not tested TAI Protein 5e-85 43
EXB16 ABD94703.1 ISPpu14 transposase Orf3 Not tested ExoU island B Protein 3e-59 42
aec53 AAW51736.1 Aec53 Not tested AGI-3 Protein 1e-80 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Mnod_6322 YP_002501420.1 transposase IS66 VFG1700 Protein 9e-81 48
Mnod_6322 YP_002501420.1 transposase IS66 VFG0793 Protein 6e-91 47
Mnod_6322 YP_002501420.1 transposase IS66 VFG1736 Protein 3e-67 43