Gene Information

Name : lysS (HAPS_0279)
Accession : YP_002474915.1
Strain : Haemophilus parasuis SH0165
Genome accession: NC_011852
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 305727 - 307178 bp
Length : 1452 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCAGAAGTCGAAAACCAAGAATTAGATCTCAATGGCGAAATGATTGCTCGCCGTGAAAAATTAGCTCAGTTGCGTGA
GCGTGGTAATCCGTTCCCGAACACATTCCGTCGTGAAGATAAAGCTGAAGATCTTCACGCAAAATATGATGAAGTTGATG
GCGAAGCACTTAAAGCTAAAGAAATTCCTACGGTAGTTGCAGGGCGTATTATGTTACGTCGTGTGATGGGTAAAGCGACA
TTTGTTACTATCCAAGATGTAAGTGGTCAGATCCAGCTTTATGTTGCACGTGATAATTTGCCTGAAGGTGAATATGAAAA
CACGGTGGGTTTATTAGATTTAGGCGATATTATCGGCGTAAAAGGCACACTATTTAAAACCAAAACAGGTGAATTAACCG
TTCGTGCAGCTGAATTACAACTGCTAACCAAAGCACTTCGTCCGTTACCAAATAAACATAAAGGCTTAACAGACCAAGAA
ACTCGCTATCGTCAGCGTTATTTAGATCTTATTGCGAACGAAGAATCTCGCCGTGCCTTTGTAATCCGCTCAAAAGTGGT
GTCAGGTATCCGTCAATTCTTCTTAGAGAAAGATTTTATCGAAGTGGAAACGCCGATGTTGCAGGTGATCCCAGGTGGCG
CAGCGGCAAAACCCTTTATTACTCACCATAACGCATTAGATGTGGATATGTATCTGCGTATCGCCCCAGAGCTTTACTTA
AAACGCCTTGTGGTTGGCGGTTTTGAGCGTGTATTCGAGTTAAACCGTAACTTCCGTAATGAAGGGGTGTCTGTTCGTCA
CAATCCTGAATTTACAATGATTGAATACTACCAAGCCTATGCAGATTACCACGATTTAATGGATAACACCGAAGAATTAT
TACGCAAATTAGCCTTAGATATTTTAGGTACAACGACCGTGCCTTACGGCGAATATGTGTTTGATTTCGGTAAACCGTTT
GAACGTATCACAATGCACGAAGCGATTGTGAAATATGGCAACGGTATTTCAATGGACGACCTACACGATTTCGACAAAGC
AGTGGCAATTGCCAAAGGCTTAAATATCGAAATTCAAAAATCGTGGGGTTTAGGTTCTATCGTCAATGCGATTTTTGAAG
AAGTAGCAGAGCATCAATTAATTCAGCCGACCTTCTTAATGGCTCACCCAGCGGAAATTTCACCGCTTGCACGCCGTAAT
GATGAAAATCCAGAAGTGACAGACCGTTTTGAATTATTTATCGGCGGACGTGAAATCGGTAACGGCTTCTCAGAATTAAA
TGATGCTGAAGATCAAGCGGAACGTTTCCAAGCACAAGTTGCAGCAAAAGAGGCGGGTGATGATGAAGCGATGTTCTACG
ATGACGATTTCGTTGTCGCCCTTGAACACGGTTTACCACCAACAGCAGGCGAAGGCTTAGGTATCGACCGCTTTAGCGAT
GATTTTCGCTAA

Protein sequence :
MSEVENQELDLNGEMIARREKLAQLRERGNPFPNTFRREDKAEDLHAKYDEVDGEALKAKEIPTVVAGRIMLRRVMGKAT
FVTIQDVSGQIQLYVARDNLPEGEYENTVGLLDLGDIIGVKGTLFKTKTGELTVRAAELQLLTKALRPLPNKHKGLTDQE
TRYRQRYLDLIANEESRRAFVIRSKVVSGIRQFFLEKDFIEVETPMLQVIPGGAAAKPFITHHNALDVDMYLRIAPELYL
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEYYQAYADYHDLMDNTEELLRKLALDILGTTTVPYGEYVFDFGKPF
ERITMHEAIVKYGNGISMDDLHDFDKAVAIAKGLNIEIQKSWGLGSIVNAIFEEVAEHQLIQPTFLMAHPAEISPLARRN
DENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFQAQVAAKEAGDDEAMFYDDDFVVALEHGLPPTAGEGLGIDRFSD
DFR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 4e-146 62

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_002474915.1 lysyl-tRNA synthetase VFG1668 Protein 2e-146 62