Gene Information

Name : lysS (CKR_0147)
Accession : YP_002470612.1
Strain : Clostridium kluyveri NBRC 12016
Genome accession: NC_011837
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 165913 - 167421 bp
Length : 1509 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGGCAAAAGAGGAAAAAAATTTACATGTACTGGAAGAGAATTTCAATCAATTGATAAGGGAGAGGATACAAAAGTTTAA
AAATTTACAAGAGCAAAATAAAGATCCCTTTGAGGTATATAAGGTTGAGAGAACCCATACTTCAAAAGAAATAAAAGATA
ACTATGAAACTTTAGATGGCACGGATGTTACTGTGGCTGGAAGACTAATGTCTAAAAGAGTTCATGGAAAGGCTGGATTT
TCAGATTTGTATGATAGATATGGTAAAATTCAACTGTATATAAAGATAAATGATGTAGGAGAAGAAAAATTAAAGGAATA
TAAATCCTATGATATAGGAGACATTTTATCTGTAAGTGGCAAGGTTTTTAAGACTAGGACCGGAGAAGTATCAATTCATA
TTACGGATTTTCAACTTGCAGCTAAATCACTTAAACCTCTTCCAGAGAAATGGCATGGACTTAAGGATCCAGATTTAAGG
TATAGACAAAGGTATGTAGATTTGATAATAAATCAGGATGTTAGAGATACCTTTTTAAAGAGGACTGCTGTAATAAGAGC
TATAAGAGAATTTTTAGATGGTAGAGATTACATAGAGGTAGAGACACCAATACTGTCATCAATAGCTGGAGGAGCAGCTG
CTAAGCCATTTATAACTCATCATAATGCTTTGGATATAGACATGTATTTAAGAATAGCAACTGAATTATATTTAAAAAGG
CTAATAGTAGGTGGATTTGAAAAGGTATATGAGATAGGTAAAAATTTTAGAAACGAAGGAATAGATGTAAGGCATAATCC
TGAATTTACAGCTATGGAATTATATGAAGCTTTTTCAGATTATAATGATATGATGGAATTAACTGAGAATATGCTGGCAT
ATGTATGTGAAAAGGTGCTTGGTACTACAAAAGTTATATATCAGGATACGGAAATAGACTTTAAGCCTCCTTGGAATAGA
ATTACTATGGTAGATGCAGTTAAACAATTTACGAAAGTAGATTTTAATGAAGTTGAAAGTGATGACGAAGCTAGAAAGAT
TGCTGTTGAAAAAAATATTGAACTTAAAAAAGAATTAAAGGATTGTACAAAAGGGGATATATTAGTAGGAATGTTTGAAG
AATTTTGTGAACATAAATTTATTCAACCAACTTTTGTAATGGATTATCCTGTTGAAATATCACCTTTAACTAAAAAGAAA
AGAGGAAATGATAAATATACTGAGAGATTTGAGGGCTTTATATTTGGTAGAGAGGTATGTAATGCATATTCAGAGTTAAA
TGATCCTATAGTGCAGAGGGAAAGATTTATGCAGCAGATAAGAGAAAGAGAATTAGGGGATGATGAAGCTTATATGATGG
ATGAAGATTTTATAAATGCTCTTGAAATAGGTATGCCTCCTACGGGAGGACTTGGAATAGGTCTGGATAGAATTATAATG
TTTCTTACGAATTCTTATTCTATAAGAGATGTCATATTATTTCCTACTATGAAGCCAAGTCAACAGTAG

Protein sequence :
MAKEEKNLHVLEENFNQLIRERIQKFKNLQEQNKDPFEVYKVERTHTSKEIKDNYETLDGTDVTVAGRLMSKRVHGKAGF
SDLYDRYGKIQLYIKINDVGEEKLKEYKSYDIGDILSVSGKVFKTRTGEVSIHITDFQLAAKSLKPLPEKWHGLKDPDLR
YRQRYVDLIINQDVRDTFLKRTAVIRAIREFLDGRDYIEVETPILSSIAGGAAAKPFITHHNALDIDMYLRIATELYLKR
LIVGGFEKVYEIGKNFRNEGIDVRHNPEFTAMELYEAFSDYNDMMELTENMLAYVCEKVLGTTKVIYQDTEIDFKPPWNR
ITMVDAVKQFTKVDFNEVESDDEARKIAVEKNIELKKELKDCTKGDILVGMFEEFCEHKFIQPTFVMDYPVEISPLTKKK
RGNDKYTERFEGFIFGREVCNAYSELNDPIVQRERFMQQIRERELGDDEAYMMDEDFINALEIGMPPTGGLGIGLDRIIM
FLTNSYSIRDVILFPTMKPSQQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 8e-106 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_002470612.1 lysyl-tRNA synthetase VFG1668 Protein 5e-106 47