Gene Information

Name : lysS (Mpal_2116)
Accession : YP_002467137.1
Strain : Methanosphaerula palustris E1-9c
Genome accession: NC_011832
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 2247834 - 2249369 bp
Length : 1536 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTGTCCACATGAGAGCCTGAATTTTGATGCAGGCAGACTTGAGAAGATCGAGGCCCTGTCAGCAGGGCATCACCAACT
CTATCCGTACAGTTTTGACCGGACCACCTCAATCTGCGAGATCAAGTCCCGCTTCGCTGACATCGGTCATGAGAAGAGCG
AGGAGTCGGTCAAGATCGCAGGCCGGATCTACACGATTCGCAACCATGGGAAGACCGTCTTCGCCGACCTTGGGGATGAA
CAGGGCCGGATCCAACTCTATATCAGAAAGAACGATGTAGGTGAGGAACAATTCTCTCTCTTCACCGAGCAGATCGACCG
CGGAGATATCATCGGGGTCAGTGGTCATCTCTTCCGGACCAAACTCGGGGAGATCACCCTCTGGGTAGACCAGTTGACCC
TGCTCACCAAGTCGCTCTGCCCGCTCCCGGAGAAGTTCCATGGGCTCAAGGACACCGAGACCCGGTACCGACAGCGGTAC
GTGGATCTTATCATGAATGAGAAGGCCCGGGAGACATTTCGTGTTCGGTCCAGGATCATTTCGCTGATCCGGCAGTTCCT
GGGCGAACGGGGTTTCCTCGAGTTTGAGACTCCGACGCTCCAACCGGTCTATGGTGGTGCGAATGCACGACCGTTCACCA
CCTATCACAACTTCCTCGATCAGACCTTCTTCCTCCGGATCGCTACGGAGTTATACCTGAAGCGGCTACTGGTCGGCGGG
TTTGAGAAGATCTTTGAGATCGGGAAGAATTTCCGGAATGAGGACATCGACACCAGTCATAACCCGGAGTTCACATTGAT
CGAGATCTATGAGGCGTACCGGGATTACAACGACATGATGGACCTGATGGAGGAGTTCCTCTCCTCACTGGTCAATGCCA
TCCATGGGTGTGACCAGGTCACGTTCGATGAGGTTGTCATCTCCTTTGCAGGACCGTGGCGGCGGATCACGATGGAGGAG
GCAGTCAGGGAGTATGCAGGGATCGATGTCCTTGCCACCTCGCTCGAAGAACTGCAGGCTTTTGCGATCGAACACGCCGT
CCCCGAGTACGAGAAGGCCGCCTCCCCCCGGGAGTTCCTGGTTCTCTTCTTCGAGTTCTTCGCAGAGAAGCAACTGATCC
AGCCGACCTTCGTCCACGACTTCCCCATTGAGAACTCCCCGCTCGCCAAGCGGCACAGATCCAAGGAGGGGTTCACCGAA
CGGTTCGAACTCTTCATCAACGGGATGGAGATCGCCAATGGTTTCTCAGAACTGAACGATCCCATCGATCAGATGAACCG
GCTCGAGGCTCAGGACAGAAAGAGGTTGTCCGGGGATCTCGAAGCCCAGATGGTCGACTACGACTTCATCAACGCGCTTG
GGTACGGGATGCCCCCAACCGGTGGTGTCGGGTTTGGTGTCGACCGGGTGGTAATGCTGCTGACCAACAACACCTCGATC
AAGGAGGTGATCCTCTTCCCGTCGATGAAGAAACCAGTCGCAGATGCACCTCCAAATGAGGACGAATCGCAAGAAAAGCC
GGCACCTCCGGCCTGA

Protein sequence :
MCPHESLNFDAGRLEKIEALSAGHHQLYPYSFDRTTSICEIKSRFADIGHEKSEESVKIAGRIYTIRNHGKTVFADLGDE
QGRIQLYIRKNDVGEEQFSLFTEQIDRGDIIGVSGHLFRTKLGEITLWVDQLTLLTKSLCPLPEKFHGLKDTETRYRQRY
VDLIMNEKARETFRVRSRIISLIRQFLGERGFLEFETPTLQPVYGGANARPFTTYHNFLDQTFFLRIATELYLKRLLVGG
FEKIFEIGKNFRNEDIDTSHNPEFTLIEIYEAYRDYNDMMDLMEEFLSSLVNAIHGCDQVTFDEVVISFAGPWRRITMEE
AVREYAGIDVLATSLEELQAFAIEHAVPEYEKAASPREFLVLFFEFFAEKQLIQPTFVHDFPIENSPLAKRHRSKEGFTE
RFELFINGMEIANGFSELNDPIDQMNRLEAQDRKRLSGDLEAQMVDYDFINALGYGMPPTGGVGFGVDRVVMLLTNNTSI
KEVILFPSMKKPVADAPPNEDESQEKPAPPA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 3e-96 44

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_002467137.1 lysyl-tRNA synthetase VFG1668 Protein 2e-96 44