Gene Information

Name : Dhaf_4914 (Dhaf_4914)
Accession : YP_002461344.1
Strain : Desulfitobacterium hafniense DCB-2
Genome accession: NC_011830
Putative virulence/resistance : Unknown
Product : rRNA large subunit methyltransferase
Function : -
COG functional category : S : Function unknown
COG ID : COG1576
EC number : -
Position : 5237111 - 5237593 bp
Length : 483 bp
Strand : -
Note : SPOUT methyltransferase; crystal structure shows homodimer; in Escherichia coli this protein methylates pseudouridine at position 1915 of the 23S ribosomal RNA

DNA sequence :
ATGCTGCAGATTAAGATCGTGGCGGTAGGAAAGATCCGGGAAAGATTCCTTATGGAAGGGATTAAGGAATATGCTAAGCG
TTTAAGTGCTTATATCCGCCTGGAGATGACGGAGATTGCCGATGAACCTTGTCCGGAGCGTCTTTCCGCCGCTGATGAAG
AGCGGGTCAAAGATAGAGAAGGTGAACGGCTTCTGAAAGGGATCGGCCCTCAGGAGCATGTTATTCTCCTTGATTTGCAG
GGAAAAGAATTCACTTCGCCGGACTTCTCTGAATATATGGATGAACTTGCCTTAATGGGAAAAAGCAGCGTAACTTTTAT
CATCGGCGGTTCCTTGGGGGTCTCTGGGGAAGTAAGGAAAAGAGCGGATTACCGCTGGTCTTTTTCCCGTCTGACTTTCC
CCCACCCTCTGATGAGGCTGATGCTGCTGGAGCAGATCTATCGGGCCATGCGCATCAGCAAGGGGGAGCCTTATCATAAA
TAG

Protein sequence :
MLQIKIVAVGKIRERFLMEGIKEYAKRLSAYIRLEMTEIADEPCPERLSAADEERVKDREGERLLKGIGPQEHVILLDLQ
GKEFTSPDFSEYMDELALMGKSSVTFIIGGSLGVSGEVRKRADYRWSFSRLTFPHPLMRLMLLEQIYRAMRISKGEPYHK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
orfX YP_251938.1 rRNA large subunit methyltransferase Not tested SCCmec Protein 6e-35 48
unnamed ACL99851.1 hypothetical protein Not tested Type-V SCCmec Protein 8e-24 48
orfXhom BAB83497.1 open reading frame X Not tested SCC 12263 Protein 2e-31 46
SSP0026 YP_300116.1 rRNA large subunit methyltransferase Not tested SCC15305RM Protein 2e-33 46
SE0023 NP_763578.1 rRNA large subunit methyltransferase Not tested SCCpbp4 Protein 4e-34 45
SERP2529 YP_190070.1 rRNA large subunit methyltransferase Not tested Type-II SCCmec Protein 4e-34 45
unnamed BAC57492.1 hypothetical protein Not tested Type-IIIinv SCCmec Protein 1e-34 44
SAV0024 NP_370548.1 rRNA large subunit methyltransferase Not tested Type-II SCCmec Protein 2e-34 44
unnamed BAB72121.1 hypothetical protein Not tested Type-IVa SCCmec Protein 1e-34 44
unnamed BAB72138.1 open reading frame X Not tested Type-IVb SCCmec Protein 1e-34 44
unnamed BAC67575.1 open reading frame X Not tested Type-IVc SCCmec Protein 1e-34 44
unnamed BAA82243.1 orfX Not tested Type-II SCCmec Protein 1e-34 44
orfX AAL26648.1 unknown Not tested SCCcap1 Protein 1e-33 44
unnamed BAD24821.1 conserved hypothetical protein orfX Not tested Type-V SCCmec Protein 1e-34 44
orfX BAA86653.1 open reading frame X Not tested Type-I SCCmec Protein 1e-34 44
unnamed BAG06184.1 conserved hypothetical protein orfX Not tested Type-VII SCCmec Protein 1e-34 44
unnamed BAB47681.1 open reading frame X Not tested Type-III SCCmec Protein 1e-34 44
unnamed BAC53844.1 open reading frame X Not tested SRImec-III and SCCmec-III region Protein 1e-34 44
SAR0024 YP_039501.1 rRNA large subunit methyltransferase Not tested Type-II SCCmec Protein 2e-34 44