Gene Information

Name : Dhaf_3967 (Dhaf_3967)
Accession : YP_002460416.1
Strain : Desulfitobacterium hafniense DCB-2
Genome accession: NC_011830
Putative virulence/resistance : Virulence
Product : AMP-dependent synthetase/ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 4217306 - 4218925 bp
Length : 1620 bp
Strand : -
Note : PFAM: AMP-dependent synthetase and ligase; KEGG: ckl:CKL_1504 predicted aryl acid activating protein

DNA sequence : 
ATGACTGGAAATCTGAAGGAGAGTCTGGAAAGCTTCTATGCTGCCAAGGTGTGGGAAGATATCACCCTGGGCAAAGCGCT
GTCGGCTTGGAGTGAAACATATGGGGACAACATCGCCTTAACCGAAGCGGACAGACAAGTAACCTATCGGGAGCTGGAAA
CGGCAGCCAGGAGAATGGCGGCCGGTTGGCAAAGGCGGGGATTTGGCAGGGGGGATAAGATCGTCCTGCAGCTTCCCAAC
TCCATTGAGTTTGTCGTCAGCGCTTTTGCCCTGTTCAAATTAGGAGTTATTCCCGTCATGGCTCTGCCTGCCCAAAGAAA
AACAGAAATCAAAGGGATTATCGAAAAATCCGGGGCCAAGGGCTATGTGATCAAGGAAAAATACTTGGGGTTTGACTATA
AAAGCCTGGCCAGGGAAATCCGCCGGGAAATCGAAACCGATCTGGCGGTGATCGTCATCGGCAGAAGCGACGAATTTATA
GCTTATGAGGATTTAGGGGAAGGCCCGGAATTAAGAGATGAACCGGAGATAGGCTCCGGGGAGGTGGGTTTGTTTTTATT
ATCGGGAGGAACAACGGGAATCCCCAAGCTGATCCCCCGAAGGCATGCCGATTATTTATATGTGGCGCAAAAGACTGCGG
AGCGCTGCCGCCTGGATCAGGAGTCCGTCTATTTGGCGGCCCTGCCTGTGGCCCACAATTTCCCCCTGGGTTGTCCGGGT
TTGCTGGGTACCTTGAGTGTGGGCGGCAGAGTCGTGATTTGTGCGGTGGCCAGCCCGGATGAAATCATCCCCCTGATTGA
AGAGGAAGGGGTGACCATAACGGGCCTGGTGCCGGCCCTGGCCCATATGTGTATTGAGTTTTTGGAGCTGGATGGGGAGT
GCGATATATCCTCCCTTAAAGTCATCCAGGTGGGGGGAGCGGTCCTGGATTCCTATTTAGCCGCCAGGATCGAAAAGGCT
TTTGCTTGCACCTTGCAGCAAATATTCGGAATCGCCGAAGGCTTGATTTGTTGTACGGATTTGGCAGACAGGGAGGAGAT
CAGGTATCACACCCAAGGGAAACCCATCTCGGCATACGATGAAATACTGATTGTGGATGAAAAAGGCCAGGAGGTCCCGG
AGGGAGAGTATGGTGAGCTGACGGTCAGGGGCCCCTATACCATCTATGGCTACTATAATCTTGAAGAAGTCAACCAAAGC
TGCCTGTCGGATCAGGGCTATTTTAAAACCGGAGACAAAGCGCGCTGGCAGGATGGCAATCTCCAGGTGGCAGGAAGGCT
GAAAGAGATGATCAACCGGGCCGGAGAGAAAATCACCCCGTCGGAGCTGGAAGAGCTGCTGCTGAGACATGAAGACATAG
AGGATGTTCAGGTGGTGGGGGTTGAGGATCAAGAGCTGGGGGAGAGGATTTGCGTATTTATAATGGCTCAGAATAAGGGG
TTGACCTTGAATGCTCTGCGCAATTATTTAGTGGGGCAGCAGGTGGCTTCATACAAACTGCCGGATCAGCTGGTTTATTT
GGAGGCCTGGCCCCTCACCAATGTAGGGAAAATCGACAGAAACCAATTAAGAGTCTTGGGAACACAGAGAGGTAATCAGG
AGGTTTTTCATGGAAGATAA

Protein sequence : 
MTGNLKESLESFYAAKVWEDITLGKALSAWSETYGDNIALTEADRQVTYRELETAARRMAAGWQRRGFGRGDKIVLQLPN
SIEFVVSAFALFKLGVIPVMALPAQRKTEIKGIIEKSGAKGYVIKEKYLGFDYKSLAREIRREIETDLAVIVIGRSDEFI
AYEDLGEGPELRDEPEIGSGEVGLFLLSGGTTGIPKLIPRRHADYLYVAQKTAERCRLDQESVYLAALPVAHNFPLGCPG
LLGTLSVGGRVVICAVASPDEIIPLIEEEGVTITGLVPALAHMCIEFLELDGECDISSLKVIQVGGAVLDSYLAARIEKA
FACTLQQIFGIAEGLICCTDLADREEIRYHTQGKPISAYDEILIVDEKGQEVPEGEYGELTVRGPYTIYGYYNLEEVNQS
CLSDQGYFKTGDKARWQDGNLQVAGRLKEMINRAGEKITPSELEELLLRHEDIEDVQVVGVEDQELGERICVFIMAQNKG
LTLNALRNYLVGQQVASYKLPDQLVYLEAWPLTNVGKIDRNQLRVLGTQRGNQEVFHGR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 5e-101 46
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 4e-102 46
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 8e-101 46
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-101 46
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 7e-101 46
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-101 46
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-101 46
ybtE CAA21394.1 - Virulence HPI Protein 7e-101 46
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-102 46

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Dhaf_3967 YP_002460416.1 AMP-dependent synthetase/ligase VFG0168 Protein 2e-94 44
Dhaf_3967 YP_002460416.1 AMP-dependent synthetase/ligase VFG1817 Protein 4e-85 42