Gene Information

Name : lysS (BCG9842_B5232)
Accession : YP_002443624.1
Strain : Bacillus cereus G9842
Genome accession: NC_011772
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 80301 - 81800 bp
Length : 1500 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGGATAACATGAACCACGAAGAATTAAACGACCAATTGCTTGTTCGTCGTGAAAAGCTACATAATTTACGTGAGCAAGG
AATCGATCCGTTCGGTAAACGATTTGAACGCACAAATTCAACGACCGACTTAGTAAGTTTATATGGAGAATTCTCTAAAG
AAGAATTAGAAGAGAAAGAAATCACTGTTTCTATCGCTGGTCGTATTATGACAAAACGCGGTAAAGGAAAAGCTGGATTC
GCGCACATTCAAGATTTACACGGACAAGTTCAAATTTACGTTCGTAAAGATACTGTTGGAGATGAAGAGTACGAGTTATT
TACGACAGCAGATTTAGGTGACTTAGTAGGTATTGAAGGTAAAGTGTTCAAAACAAACGTTGGAGAACTTTCAGTAAAAG
CAACGGGATTTACGCTTTTAACGAAATCTCTCCGTCCGTTACCGGATAAATATCATGGATTAAAAGATGTTGAACAACGA
TACCGTCAACGTTACTTAGACTTAATTACAAGTATGGAAAGTCGTGAAACATTTGTTACTCGCAGTAAAATTATTCGTGA
AATGAGAAGATATTTAGATGACAACGGTTATCTTGAAGTAGAAACGCCTATGATGCACGCGATTGCAGGTGGAGCATCTG
CTCGTCCTTTCACTACGCATCATAATGCGTTAGATATGGAATTATATATGCGCATCGCAATTGAACTTCATTTAAAACGT
CTTATTGTGGGCGGATTAGAAAAAGTTTATGAGATTGGCCGTGTATTCCGTAATGAGGGTGTATCAACTCGTCATAACCC
TGAGTTTACGATGATTGAATTATATGAAGCGTACGCTGATTATAATGATATTATGAAACTAACAGAAAATATGGTTGCTC
ATATTGCGAAAAAAGTATTGGGTACAACAACAATCCAATATGGTGATTATGAAATTAATCTAGAACCAGAATGGACACGT
CTTCATATGGTAGATGCAATTAAGCAACACTCTGGAGCGGATTTCTGGAATCCGATGAGTGTAGAAGAAGCGCGTGAGCT
TGCGAAAGAACATAATGTAGAAATTAAGGATACAATGGAAGTTGGTCATATTATTAATGAGTTCTTCGAGCAAAAAGTAG
AAGATAAATTAATCCAACCAACATTTATTTACGGTCATCCGGTAGAAATTTCGCCACTTGCGAAAAAGAATGATGAAGAT
CCACGATTCACAGATCGTTTCGAATTATTTATCGTTGCACGTGAACATGCAAATGCATTTACTGAGTTAAATGATCCGAT
CGATCAAAAAGAACGTTTTGAAGCGCAATTAAAAGAGCGTGAACAAGGTAATGACGAAGCACATATGATGGATGACGATT
ATATTGAAGCTCTTGAGTACGGTATGCCTCCTACGGGTGGATTAGGAATTGGTATTGATCGTCTAGTTATGTTATTAACA
AATGCACCATCTATTCGTGATGTATTATTATTCCCTGCTATGCGCCATAAACAAGACTAA

Protein sequence :
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTNSTTDLVSLYGEFSKEELEEKEITVSIAGRIMTKRGKGKAGF
AHIQDLHGQVQIYVRKDTVGDEEYELFTTADLGDLVGIEGKVFKTNVGELSVKATGFTLLTKSLRPLPDKYHGLKDVEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFTTHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYNDIMKLTENMVAHIAKKVLGTTTIQYGDYEINLEPEWTR
LHMVDAIKQHSGADFWNPMSVEEARELAKEHNVEIKDTMEVGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDED
PRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NAPSIRDVLLFPAMRHKQD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-121 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_002443624.1 lysyl-tRNA synthetase VFG1668 Protein 1e-121 51