Gene Information

Name : lysS (PLES_12841)
Accession : YP_002438887.1
Strain : Pseudomonas aeruginosa LESB58
Genome accession: NC_011770
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 1395117 - 1396622 bp
Length : 1506 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGAGCGACCAACAACTCGACCAGCACGAACTGCAACAGGAAGAAAACAAGCTGATCGCCCAGCGCAAGGAAAAACTTGC
CGCCGTGCGCGAAGCCCGGGCCATCGCCTTCCCCAACGACTTCCGCCGCGACGCCTACTTCGCGGACCTGCAGAAACAGT
ACGCCGACAAGACCAAGGAAGAGCTGGAAGCCGCAGCGATCCCGGTCAAGGTGGCCGGTCGGATCATGCTCAACCGCGGT
TCCTTCATCGTCCTCCAGGACAGCAGCGAGCGCCTGCAGGTCTACGTCAACCGCAAGACCCTGCCGGAAGAAACCCTGGC
CGAGATCAAGACCTGGGACCTGGGCGACATCATCGGCGCCGAAGGCGTGCTGGCCCGCTCGGGCAAGGGCGACCTGTACG
TCGACATGACCAGCGTGCGCCTGCTGACCAAGTCGCTGCGTCCGCTGCCGGACAAGCACCATGGCCTGACCGACACCGAG
CAGCGCTACCGCCAGCGCTACGTCGACCTGATGGTCAACGAGGAAACCCGCCACACCTTCCGCGTGCGTTCCCAGGTCAT
CGCCCACATCCGCCGTTTCCTCAGCGAGCGCGGTTTCCTCGAAGTGGAGACCCCGATGCTGCAGACCATCCCCGGCGGCG
CCGCGGCCAAGCCGTTCGAGACCCATCACAACGCGCTGGACATGGCCATGTTCCTGCGCATCGCCCCGGAGCTGTACCTC
AAGCGCCTGGTGGTCGGCGGTTTCGAGAAGGTCTTCGAGATCAACCGCAACTTCCGTAACGAAGGCGTCTCGACCCGGCA
CAACCCCGAGTTCACCATGCTCGAGTTCTACCAGGCCTACGCCGACTACGAAGACAACATGGACCTGACCGAGGAACTGT
TCCGCGAACTGGCCCAGGCGGTACTCGGTACCACCGACGTGCCCTACGGCGACAAGGTCTTCCACTTCGGCGAGCCGTTC
GTGCGCCTGTCGGTGTTCGATTCGATCCTCAAGTACAACCCGGAGATCACCGCCGCCGACCTCAACGACGTCGAGAAGGC
GCGGGCCATCGCCAAGAAGGCCGGCGCCAAGGTCCTCGGCCACGAAGGCCTGGGCAAGCTGCAGGTGATGATTTTCGAGG
AACTGGTGGAGCACAAGCTGGAGCAGCCGCACTTCATCACCCGCTACCCCTTCGAGGTTTCGCCGCTGGCCCGCCGCAAC
GACGAAGACCCGAGCGTCACCGACCGCTTCGAGCTGTTCATCGGCGGCCGCGAGATCGCCAATGCCTATTCCGAGCTGAA
CGACGCCGAGGACCAGGCCGAGCGCTTCATGTTGCAGGTCAAGGAGAAGGACGCCGGCGACGACGAGGCGATGCACTACG
ACGCCGACTTCATCAATGCCCTGGAATACGGCATGCCGCCGACCGCCGGCGAAGGCATCGGCATCGACCGCCTGGTGATG
CTGCTGACCAACTCGCCGTCGATCCGCGACGTGATCCTGTTCCCGCACATGCGCCCGCAGGCCTGA

Protein sequence :
MSDQQLDQHELQQEENKLIAQRKEKLAAVREARAIAFPNDFRRDAYFADLQKQYADKTKEELEAAAIPVKVAGRIMLNRG
SFIVLQDSSERLQVYVNRKTLPEETLAEIKTWDLGDIIGAEGVLARSGKGDLYVDMTSVRLLTKSLRPLPDKHHGLTDTE
QRYRQRYVDLMVNEETRHTFRVRSQVIAHIRRFLSERGFLEVETPMLQTIPGGAAAKPFETHHNALDMAMFLRIAPELYL
KRLVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYEDNMDLTEELFRELAQAVLGTTDVPYGDKVFHFGEPF
VRLSVFDSILKYNPEITAADLNDVEKARAIAKKAGAKVLGHEGLGKLQVMIFEELVEHKLEQPHFITRYPFEVSPLARRN
DEDPSVTDRFELFIGGREIANAYSELNDAEDQAERFMLQVKEKDAGDDEAMHYDADFINALEYGMPPTAGEGIGIDRLVM
LLTNSPSIRDVILFPHMRPQA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 3e-136 59
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 6e-78 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_002438887.1 lysyl-tRNA synthetase VFG1668 Protein 2e-136 59