Gene Information

Name : AFE_0447 (AFE_0447)
Accession : YP_002424943.1
Strain : Acidithiobacillus ferrooxidans ATCC 23270
Genome accession: NC_011761
Putative virulence/resistance : Virulence
Product : sigma-54 dependent DNA-binding response regulator
Function : -
COG functional category : T : Signal transduction mechanisms
COG ID : COG2204
EC number : -
Position : 387060 - 388445 bp
Length : 1386 bp
Strand : -
Note : identified by match to protein family HMM PF00072; match to protein family HMM PF00158; match to protein family HMM PF07728

DNA sequence :
ATGCCCAGACCAGCTCCCATCATCCTGACCATCGACGATGATCCCGACTTTCTGCGCCTGCTCGGCCTCTGGCTGGAAAG
TGAGGGATACCACGTCCTGGCCAGCAGTGATCCCGTGCAGGGCATGCAGACGTTGCAGACGGCCAGACCGGATCTGGTCA
TCACCGATCTGCGCATGCCCGGCATGGATGGAATGGCCGTGCTGGAGACGGTGCAGGCCCTCGATCCCGACCTCCCCGTT
ATCCTCCTCACCGCCCACGGCAGCATCCCCAACGCCGTGGCAGCCATGCGTGCCCAGGCTTTCGGATATCTGAGCAAACC
CTTCAGCAATGAAGAGCTCCAGGAGTTGACCACCGCCGCCCTGGCGCAGCGCCACGCCGGGCAGGAAACCCGCAAGCTCC
GGGCGGCGTTGCGGCAACAGGCCGGGCAGAGCATCCTCCATCGTAGCCCCGCCATGGCGGCGCTGGTGGATGAGCTGGCG
TGCGTTGCCAACTCGAAAGCCAGCGTTTTCCTTTCCGGAGAAAGCGGATCCGGCAAAGAACGGGTCGCTCGTGCCATCCA
CGACGCCAGCCCACGCCGTGACGGCCCGTTCGTTGCGGTCAATTGCGGTGCCATCCCCGCCGGGCTGGCGGAGAGCGAAC
TCTTCGGCCATGTCAAGGGCGCCTTCACCGGCGCCACCCAGGATCACACCGGATTGATCCGCAGCGCCAGCGGCGGCACT
CTGTTCCTCGACGAAATCGCCGACCTGCCTCTCACTCTGCAGGTCAAATTGCTCCGCGTCCTTCAGGAAGGTTCCTTGCG
CCCCGTCGGCGCCAAAACGGAAATTTCCGTCGACCTGCGGGTCATCAGTGCCACCCATCAGGACATTCACGCCCTCACCG
CCGACGGCACCTTCCGCGAGGATCTTTACTATCGCCTGCATGTCATCCCGCTGCGGGTCCCCAGCCTGAGCGAAAGGGCG
GAAGACATCCCGCTGCTGGCCCAGCACTTTCTGGACCGCGAGAGTCAGCGCATGAACCGGGATATCCTGGGTTTCAGTCC
CGAGGCCCTCGACAAGCTGATGCAACGATCCTGGCCCGGCAACGTCCGGGAATTGGAGAATGCCGTCACCTTTGCCGCGG
CCGTCACCGAAAAAGGCTGGGTCGCCGCCGACGCCATCCCTGACAACGGCCGCCAGGGTGGCCAGAGCGCCTTTCCTCCC
CTGCAGGACGCCAAGGCGGCCTTTGAGCGCGGCTACCTGGAAAACCTGCTACGCGCGACGGATGGCAACATCTCCCGTGC
CGCACGCATTGCCGGCCGACACCGGACGGATCTTTACAAGCTGATGCGTAAGCACAGTCTGGCTCCGCAACTTTTCAAGA
ATCCCGAGGACCGGGATGAGGAATAG

Protein sequence :
MPRPAPIILTIDDDPDFLRLLGLWLESEGYHVLASSDPVQGMQTLQTARPDLVITDLRMPGMDGMAVLETVQALDPDLPV
ILLTAHGSIPNAVAAMRAQAFGYLSKPFSNEELQELTTAALAQRHAGQETRKLRAALRQQAGQSILHRSPAMAALVDELA
CVANSKASVFLSGESGSGKERVARAIHDASPRRDGPFVAVNCGAIPAGLAESELFGHVKGAFTGATQDHTGLIRSASGGT
LFLDEIADLPLTLQVKLLRVLQEGSLRPVGAKTEISVDLRVISATHQDIHALTADGTFREDLYYRLHVIPLRVPSLSERA
EDIPLLAQHFLDRESQRMNRDILGFSPEALDKLMQRSWPGNVRELENAVTFAAAVTEKGWVAADAIPDNGRQGGQSAFPP
LQDAKAAFERGYLENLLRATDGNISRAARIAGRHRTDLYKLMRKHSLAPQLFKNPEDRDEE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
BJAB07104_00300 YP_008207765.1 Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains Not tested AbaR25 Protein 7e-64 41
BJAB0868_00304 YP_008211630.1 Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains Not tested AbaR26 Protein 7e-64 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
AFE_0447 YP_002424943.1 sigma-54 dependent DNA-binding response regulator BAC0467 Protein 4e-72 47
AFE_0447 YP_002424943.1 sigma-54 dependent DNA-binding response regulator BAC0089 Protein 3e-90 44

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
AFE_0447 YP_002424943.1 sigma-54 dependent DNA-binding response regulator VFG1249 Protein 6e-61 44
AFE_0447 YP_002424943.1 sigma-54 dependent DNA-binding response regulator VFG1214 Protein 2e-71 42