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Name : AFE_2790 (AFE_2790) Accession : YP_002427160.1 Strain : Acidithiobacillus ferrooxidans ATCC 23270 Genome accession: NC_011761 Putative virulence/resistance : Unknown Product : type I restriction-modification enzyme, R subunit Function : - COG functional category : V : Defense mechanisms COG ID : COG0610 EC number : 3.1.21.3 Position : 2480037 - 2483105 bp Length : 3069 bp Strand : - Note : identified by match to protein family HMM PF04313; match to protein family HMM PF04851; match to protein family HMM TIGR00348 DNA sequence : ATGACCCCCCTCAACGAATCCACCATCGAACACCACGCCATTGCCCTATTCAAAGAACTGGGCTACGCCTACGCATTCGG CCCCGACATCGGCCCGGATAGCAACCAAAAAGAACGCGCTGACTACGAAAACCCCCTGTTACTGGAACGCCTGCGTACAG CCATCCATCGCCTGAATCCAGAAGCCCCCAAGGAAATCCGCGACGAAGCCCTACGCCATGTGCAGGCTATTGCCGTGGGT GGACTGATGACCGAGAACAGCCTATTTCAGCAGATGCTCGTGGAAGGGGTACGGGTGGCGCACATGGTGGACGGCGAAGA ACGCGGCAGCATCGTCCGCCTGATCGACTACGACCATCCTGAAAAGAATGATTTGCTGGTGGTCAACCAGTACACCGTCA TCCACAACCGCATCAACCGCCGCGCCGATCTAGTGGTTTTCGTGAATGGCCTGCCCTTGGGCCTCTTTGAACTGAAAAAC ATGGCGGACGCCAACGCCACCACCCGCCGGGCATGGAATCAACTACAGACCTATCAGGCCGACATTCCCAACCTCATGGC CTACAACGCCATACTGGTCATCAGCGATGGGCTGAAGGCCGCCGTAGGCTGTCTGGGTGCCCCTTATGAACGCTTTCTGC CCTGGAAAACCATCGACGGTCAGGAACTCATGGACCGGGGGGATGACCCTTTACGTTTCCTGATTCACGGGGTTTTTGAA CCCGTCCGTTTTCTGGACTTGATCCGCTATTTCATCGCCATTAGTGACGATGGCCGCAAACTCAGCAAGAAGATCGCCGC CTATCATCAGTTCCACGCCGCCCAGAAGGCCCTGCTGACCACCCTGGCATCGGCAGGCATCGGTGGTGATCGCCGGGGTG GTGTGGTCTGGCATACCCAGGGGTCCGGCAAGAGCCTCACCATGCTCTTTTTCGCGGGCATGCTCATCCAGCACCCGGCG CTGGAAAACCCCACCATCCTCATGCTCACCGACCGCACGAACCTGGACACCCAGTTGTTCGATACCTTTGCCGCAGGCAA GCAGCTTTTACGGCAAGACCCGCAGCAGGCCGACAGCGTCAGTGATCTTCGGGAACTGCTGCAACGGGCTTCCGGCGGTG TACTGTTCAGCACCATCCAGAAATTCCAGAAAGACCGCGACGAACCTTTGGGCAAGCACCCGGTATTGTCCGAGCGGAAG AACATCATCGTCATGGTGGATGAAGCCCAGCGTAGCCAGTATGAGATTCTGAAGGGATATGCCGCCAATCTGCGGGCGGC CTTGCCCAACGCGACCTTCGTGGCCTTCACCGGCACACCGCTGGAACTGGATGACCGGGACACGCGGGTGGTGTTCGGCG ATTACATCGACATCTACGATATTCAGCGGGCCGTCGAGGATGGGGCCACCGTCCCGATTTATTACGAAAGCCGCCTGGTG CGCCTCAACCTGCCGGAAGACCAGCGCCCCCTCATCGACAGCACCTTTGAGGAAATCACCGAAGACGACGAGCAGGAAAA CAAGGAACGCCTGAAAACCCGCTGGGCGCAACTGGAAGCCCTGGTCGGCGCCCCCAAGCGAGTGGAACAGATTGCCGCTG ACCTGCTGGACCATTGGGAAAAGCGCAAGAGCATCCTGTCCGGCAAGGCCATGATCGTCGCCATGAGCCGTCGCATTGCC GTGGAACTTTATGATGCCATCGTCCGTATCCATCCCGACTGGCGCGGTGACGACGACACGCAAGGCCGGATCAAGGTGGT CATGACCGGTTCCGCCAGCGACCCCATCGAATGGAAGCCGCATATCCGCAGCAAGGCGGGCAATGAAGCCATTGCCGACC GCCTGAAAGACCCGGATGATCCGTTAGAAATCGTCATCGTGCGGGATATGTGGCTGACGGGCTTCGACGCCCCGGCCCTG AATACGCTCTATGTGGACAAACCCATGCGCGGGCACACCCTCATGCAGGCCATCGCCCGCGTCAACCGGGTCTTCACCAA CAAGTCCGGTGGACTGGTGGTGGATTACATCGGCATTGCCCAGGACCTGAAAAACGCCATTGCCACCTACACCCGTTCCG GTGGCAAGGGCAATCCGGCGGAAACCGTGCAGACCGCCATCAAGGTGCTGCTGGAAAAACTCGACATCTGCCGCGGTATT TTCCATGGCTGTGACTATCAGGATTATCTGACTGACGACGGATCAGAAAAGGTGGCCACCCTGCGCAAGGCCACCGAATT CATTCTGTCTCAGGAACTCACCGAAGAAGGCGTCAAGCGCCGTTTCCGCAACGCCACGTCCGGCCTCAAAAAAGCGGCGA CTTTAGCCGCCGGCGACGACATTGTGGAACGCTGCCGGACTGAGATCATTTTCTTCCTGAGCGTCCGTGCCACTCTGGAC AAGTCCAGCGAGGCCGAGTCTCTGGTGGAAGAACAGGAATACGCCATCCGCCAACTTATCGACCAATCCATCGCCCCGGA AGGCGTGGTGGACCTGTATGCGGTGGCGGGGCTACCCAAAAGCGAAATATCCCTGCTGTCCGATGAATTCATCCACCAGA TCGAGGCTATGCCCGAAAAGAATCTGGCGCTGGAAATGCTGCGTCGATTACTGCAGGACAAGGTCCGCAAGGAAGGCAAG GGCAACCTGATCCAGAGCAAAGCCTTCTCGGAAAAGCTGGAAGAAGCCCTGCGCAAATACCACAACCGTTCGGTGGATAC CGTCGAGGTGATGCAGGAACTCATCGAACTGGCCAAAGCATTACAGGCGCTGGATCAACGCCATGCGGAACTGGGCCTCA GCAAGGACGAGGCCGCCTTTTATGATGCCCTGGCGACCAACGATTCCGCCGTGCAGGCCATGGGGGACGAGGCCCTGAAA ATGATCGCCAAGGAAGTGGCCGATACTGTGCGCCAAAACACCCGCATCGACTGGTCCATCCGGGAACAGGCAAGGGCGCA TCTGCGGCGCATGGTGAAGCGGGTGCTACGCAAACACGGCTATCCACCCGACATGCAGGAAGGGGCGGTGAATCTGGTCA TTGAACAGGCTGAGGGGTTGGTGGGGTGA Protein sequence : MTPLNESTIEHHAIALFKELGYAYAFGPDIGPDSNQKERADYENPLLLERLRTAIHRLNPEAPKEIRDEALRHVQAIAVG GLMTENSLFQQMLVEGVRVAHMVDGEERGSIVRLIDYDHPEKNDLLVVNQYTVIHNRINRRADLVVFVNGLPLGLFELKN MADANATTRRAWNQLQTYQADIPNLMAYNAILVISDGLKAAVGCLGAPYERFLPWKTIDGQELMDRGDDPLRFLIHGVFE PVRFLDLIRYFIAISDDGRKLSKKIAAYHQFHAAQKALLTTLASAGIGGDRRGGVVWHTQGSGKSLTMLFFAGMLIQHPA LENPTILMLTDRTNLDTQLFDTFAAGKQLLRQDPQQADSVSDLRELLQRASGGVLFSTIQKFQKDRDEPLGKHPVLSERK NIIVMVDEAQRSQYEILKGYAANLRAALPNATFVAFTGTPLELDDRDTRVVFGDYIDIYDIQRAVEDGATVPIYYESRLV RLNLPEDQRPLIDSTFEEITEDDEQENKERLKTRWAQLEALVGAPKRVEQIAADLLDHWEKRKSILSGKAMIVAMSRRIA VELYDAIVRIHPDWRGDDDTQGRIKVVMTGSASDPIEWKPHIRSKAGNEAIADRLKDPDDPLEIVIVRDMWLTGFDAPAL NTLYVDKPMRGHTLMQAIARVNRVFTNKSGGLVVDYIGIAQDLKNAIATYTRSGGKGNPAETVQTAIKVLLEKLDICRGI FHGCDYQDYLTDDGSEKVATLRKATEFILSQELTEEGVKRRFRNATSGLKKAATLAAGDDIVERCRTEIIFFLSVRATLD KSSEAESLVEEQEYAIRQLIDQSIAPEGVVDLYAVAGLPKSEISLLSDEFIHQIEAMPEKNLALEMLRRLLQDKVRKEGK GNLIQSKAFSEKLEEALRKYHNRSVDTVEVMQELIELAKALQALDQRHAELGLSKDEAAFYDALATNDSAVQAMGDEALK MIAKEVADTVRQNTRIDWSIREQARAHLRRMVKRVLRKHGYPPDMQEGAVNLVIEQAEGLVG |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| hsdR | BAH57699.1 | type I restriction-modification system endonuclease homologue | Not tested | Type-VII SCCmec | Protein | 0.0 | 45 |
| hsdR | YP_251977.1 | type I restriction-modification system restriction subunit | Not tested | SCCmec | Protein | 0.0 | 44 |
| api52 | CAF28526.1 | hsdr-like Type I restriction enzyme | Not tested | YAPI | Protein | 0.0 | 44 |
| hsdR | BAD24840.1 | type I restriction-modification system endonuclease homologue | Not tested | Type-V SCCmec | Protein | 0.0 | 44 |
