Gene Information

Name : AFE_2470 (AFE_2470)
Accession : YP_002426853.1
Strain : Acidithiobacillus ferrooxidans ATCC 23270
Genome accession: NC_011761
Putative virulence/resistance : Unknown
Product : ISAfe4, transposase orf3
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG3436
EC number : -
Position : 2201515 - 2203152 bp
Length : 1638 bp
Strand : +
Note : identified by match to protein family HMM PF03050

DNA sequence :
ATGATTTCAGCGCCGCAAACGCCTCTTCCCACCAGCCCGGACGCCCTGCGCGATCTGGTGCTGAGCCTGCTGCAACAGCA
GGAGGAACAGGAAGCGGAACGCACCCGGATCATCGCCCAACAGCAAGCGGCCATTGCCCTGCGCGATGAAACCATCGCCC
GCCTGGAAAGCACCATCGCCAAACTGCAGCGCTGGCGATTCGGACGCCGTTCGGAAAAGCTCTCTCCCGACCAGATCAGT
CTTTGGGAAGAATCGCTGGATACCGAAATCGCGGCGATGGAAAGCCTCCTCGAAACCGTTCTGGAAGACAGTGCGGCCGT
GACCGCTGGCCGTGCAGAGGGAGCAGCCGCCGACGCGCAGACCGTAACGGTCCCCGCCCGTCCCGTACGCCGGCACCCCG
GCCGCATGGCGATCCCCGCCCATCTGCCCCGGGTAGAAGTACGTCACGATCCCAAGACTTGCACTTGCGCGCAATGTGGG
GGTCCACTCGAAACTGTCGGGGAAGAGATCAGCGAAAAACTGGATTATATCCCCGGACGCTTCCAGGTGATTCGCCATAT
CCGCCCCAAACTGGCCTGCCGCCCCTGTGGTACCATCGAAAGTCCGGCATTACCGGCACAGGTGATTGACAAGGGCTTGC
CCACGGCGCGCCTGGTGGCCCATGTGATGACGGCGAAACACGTGGATCACCTGCCTTTGTACCGGCAGGGAACCCAGTAC
CAGCGAGCGGGCGTACCGATCTCTCGCGCCACCCTCTGCAGCTGGCTGGGTCAGGGCGAATACTGGATCAGTCTGCTCGC
CGAAGCTTGCAAAATGGCCTTACTGGAAGGAAAGATTCTGCACGCCGACGAGACGCCCTTGCCCGTCCTCAACCCCGGCA
GCGGCAAGACGGACAAAGCCTATCTCTGGGTGTATCGCAGCCAGGCGGATGCCCCGCATCCCGTCGTGGTCTTTGATTAT
GCCCCGGACCGTAAGGGGATCCACGCGCAACACTTCCTGGGCGACTGGCAAGGCATCCTCCAGACCGATGACTATGGGGG
GTATGATGCCCTCTACCGCAAGGAACAGATCATCGAAGCCGGATGCTGGGCGCATGTACGCCGCCACTTCTATGACGTGG
AACAGCGCGGTCCCAGTCCGGTAGCCCAGAAAGCCCTGGCCTGGATCGTCAAACTCTACGCCATCGAAGCGGAGATCAAG
GAATCTCTACCAGATCAGAAAGTCGCCGCCCGGCAGCAGCGTGCCGGTCCCCTGCTGGAAGCCTTCCATGCCTGGCTCAC
GGAAACCCAGATGCAGGTGGCGCCGAAAAGTGGTATTGCCAAAGCGATGGCCTATGCCCTCAACCGTTGGAAAGCACTGA
CGCTCTACCTCGAAGAAGGACAACTCAGCATCGATAATAATCCGGTGGAGCGGGCGCTGCGGGGCGTGGCCATTGGTCGC
AAGAATTTTTTATTTGTTGGAAATGATGCGGGTGGTGAACGTGCCGCGTCCTTCTACAGCATTATCGAAACGTGCAAACT
CAACGGCGTCGAGCCCTTCGCTTACCTCTGCGATGTGCTGGAAAAGCTCCCGACCTGGCCCAACAAGCGGCTCCACGAAC
TTTTGCCGTGGAACTGGAAAAAAACCGCATTACCCTGA

Protein sequence :
MISAPQTPLPTSPDALRDLVLSLLQQQEEQEAERTRIIAQQQAAIALRDETIARLESTIAKLQRWRFGRRSEKLSPDQIS
LWEESLDTEIAAMESLLETVLEDSAAVTAGRAEGAAADAQTVTVPARPVRRHPGRMAIPAHLPRVEVRHDPKTCTCAQCG
GPLETVGEEISEKLDYIPGRFQVIRHIRPKLACRPCGTIESPALPAQVIDKGLPTARLVAHVMTAKHVDHLPLYRQGTQY
QRAGVPISRATLCSWLGQGEYWISLLAEACKMALLEGKILHADETPLPVLNPGSGKTDKAYLWVYRSQADAPHPVVVFDY
APDRKGIHAQHFLGDWQGILQTDDYGGYDALYRKEQIIEAGCWAHVRRHFYDVEQRGPSPVAQKALAWIVKLYAIEAEIK
ESLPDQKVAARQQRAGPLLEAFHAWLTETQMQVAPKSGIAKAMAYALNRWKALTLYLEEGQLSIDNNPVERALRGVAIGR
KNFLFVGNDAGGERAASFYSIIETCKLNGVEPFAYLCDVLEKLPTWPNKRLHELLPWNWKKTALP

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
st57 CAC81895.1 ST57 protein Not tested LEE II Protein 4e-89 46
RL059 AAP84184.1 transposase Not tested PAPI-1 Protein 2e-91 43
aec53 AAW51736.1 Aec53 Not tested AGI-3 Protein 1e-84 43
Z1131 NP_286666.1 hypothetical protein Not tested TAI Protein 4e-91 42
tnp AEA34686.1 transposase Not tested Not named Protein 5e-92 42
Z1570 NP_287074.1 hypothetical protein Not tested TAI Protein 4e-91 42
unnamed AAC31494.1 L0015 Not tested LEE Protein 4e-92 42
Z4337 NP_289562.1 hypothetical protein Not tested OI-122 Protein 6e-92 42
unnamed CAC39285.1 hypothetical protein Not tested LPA Protein 4e-92 42
Z5098 NP_290249.1 prophage-associated protein Not tested LEE Protein 6e-92 42
ECs4547 NP_312574.1 hypothetical protein Not tested LEE Protein 2e-92 42
unnamed ACU09439.1 IS66 family element transposase Not tested LEE Protein 2e-92 42
unnamed AAL57570.1 unknown Not tested LEE Protein 3e-92 42
unnamed CAI43806.1 hypothetical protein Not tested LEE Protein 2e-92 42
BCAM0248 YP_002232880.1 putative transposase Not tested BcenGI11 Protein 3e-92 42
Z4340 NP_289564.1 hypothetical protein Not tested OI-122 Protein 3e-82 41
Z1600 NP_287104.1 hypothetical protein Not tested TAI Protein 4e-84 41
Z1161 NP_286696.1 hypothetical protein Not tested TAI Protein 4e-84 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
AFE_2470 YP_002426853.1 ISAfe4, transposase orf3 VFG0793 Protein 3e-92 42