Gene Information

Name : flu (ECUMN_3400)
Accession : YP_002414067.1
Strain : Escherichia coli UMN026
Genome accession: NC_011751
Putative virulence/resistance : Virulence
Product : antigen 43 (Ag43) phase-variable biofilm formation autotransporter; CP4-44 prophage
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG3468
EC number : -
Position : 3514897 - 3518022 bp
Length : 3126 bp
Strand : +
Note : Evidence 1b : Function experimentally demonstrated in the studied species; PubMedId : 20392470, 97257509, 99212225, 2661530, 9298646; Product type h : extrachromosomal origin

DNA sequence :
ATGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATTACGGGCGCTTTCGTGGTTGCCTCCGA
ACTGGCCCGCGCACGGGGTAAACGTGGCGGTGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGG
CTGCTGACATCGTTGTGCACCCGGGTGAAACAGTGAATGGCGGAACACTGGTAAACCATGACAACCAGTTTGTATCCGGA
ACAGCTGATGGCGTGACTGTCAGTACCGGGCTTGAGCTGGGGCCGGACTCTGATGACAACACCGGCGGTCAGCAGATAGC
CCGGGGTGGCACTGCCAGAAACACCACGGTCACCGCAAACGGTCTGCAGGATGTCATGGCAGGTGGCAGTGCCACGGATA
CGGTTATCAGTGCCGGTGGAGGACAGAATCTGCGCGGGCAGGCTTACGGTACTGTCCTTAACGGTGGTGAACAATGGATA
CATGCAGGCGGCAGTGCATCCGGAACGGTGATTAACCAGTCTGGCTACCAGACCATTAAACATGGCGGGCAGGCAACCGG
AACCATCGTCAACACCGGTGCAGAAGGTGGTCCGGAGTCTGAAAATGTGTCCAGCGGTCAGATGGTCGGAGGGACGGCTG
AATCCACCACCATCAATAAAAATGGCCGGCAGGTTATCTGGTCTTCGGGGATGGCACGGGACACCCTCATTTACGCCGGC
GGTGACCAGACGGTACACGGAGAGGCACATAACACCCGGCTGGAGGGAGGAAACCAGTATGTGCACAAATACGGACTTGC
GCTGAACACGGTAATTAACGAGGGGGGCTGGCAGGTTGTTAAGGCAGGTGGTACTGCCGGTAACACCACCATAAATCAGA
ACGGCGAGCTGAAGGTTCATGCCGGCGGAGAAGCGTCTGATGTCACCCAGAACACGGGCGGTGCACTGGTTACCAGTACA
GCTGCAACCGTCACCGGCACAAACCGCCTGGGAGCATTCTCCGTTGTGGCGGGTAAGGCTGATAATGTCGTACTGGAAAA
TGGCGGACGTCTGGATGTGCTGAGCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCA
ACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCTGGTGCCGCTGTCAGTGGTACC
CGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCT
GAACGCCGGTGATACGGCCACGGATACCACGGTAAATGGCGGACTGTTCACCGCCAGGGGCGGCACACTGGCGGGCACCA
CCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGCGAT
GCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACAC
CACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATCG
CTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCC
TCTGGTGCCACCTGGAATATTCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCA
TTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCA
GCCTGCGTGTACGCCCGGATATGGCACAGAACAATGCTGACAGACTGGTCATTGACGGTGGCAGGGCAACCGGAAAAACC
ATCCTGAACCTGGTGAACGCCGGCAACAGTGCGTCGGGGCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCAT
TAACGGTGCCACCACGGAGGAAGGGGCCTTTATCCAGGGGAATAAGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACC
GGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGAAGTCCCCCTGTATGCCTCCATGCTGACA
CAGGCAATGGACTATGACCGGATTCTGGCAGGCTCCCGCAGCCATCAGACCGGTGTAAACGGTGAAAATAACAGCGCCCG
TCTCAGCATTCAGGGCGGCCATCTCGGTCACGATAACAACGGCGGTATTGCCCGTGGGGCCACGCCGGAAAGCAGCGGCA
GCTATGGCTTCGTCCGTCTGGAGGGTGACCTGCTGAGAACAGAGGTTGCCGGTATGTCACTGACGACAGGAGTGTATGGT
GCTGCAGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGCGCCGGCACGGTCCGGGATGATGCCGGCAGCCT
GGGCGGATATATGAACCTGACACACACCTCCTCCGGCCTGTGGGCTGACATAGTGGCACAGGGAACCCGCCACAGTATGA
AAGCGTCATCGGGCAATAACGACTTCCGCGCACGGGGCCGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGT
ATCACTGACAACCTGATGCTGGAGCCACGGCTGCAGTACACCTGGCAGGGGCTTTCGCTGGATGACGGTAAGGACAACGC
CGGTTATGTGAAGTTCGGGCATGGCAGTGCACAACATGTGCGTGCCGGTTTCCGTCTGGGCAGCCACAACGATATGACCT
TTGGTGAAGGCACCTCATCCCGTGCCCCCCTGCGTGACAGTGCAAAACACAGTGTGCGTGAACTGCCGGTGAACTGGTGG
GTACAGCCTTCCGTTATCCGCACCTTCAGCTCCCGGGGTGATATGCGTGTGGGGACCTCCACCGCAGGCAGCGGGATGAC
GTTCTCACCCTCACAGAATGGCACTTCACTGGTTCTGCAGGCAGGACTGGAAGCCCGTGTCCGGGAAAATATCACCCTGG
GCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAAGTTATAACAGTCAGGCCACGCTGAATGTGACC
TTCTGA

Protein sequence :
MLMKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSG
TADGVTVSTGLELGPDSDDNTGGQQIARGGTARNTTVTANGLQDVMAGGSATDTVISAGGGQNLRGQAYGTVLNGGEQWI
HAGGSASGTVINQSGYQTIKHGGQATGTIVNTGAEGGPESENVSSGQMVGGTAESTTINKNGRQVIWSSGMARDTLIYAG
GDQTVHGEAHNTRLEGGNQYVHKYGLALNTVINEGGWQVVKAGGTAGNTTINQNGELKVHAGGEASDVTQNTGGALVTST
AATVTGTNRLGAFSVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSGAAVSGT
RSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGAILTLSGKTVNNDTLTIREGD
ALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLA
SGATWNIPDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKT
ILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLT
QAMDYDRILAGSRSHQTGVNGENNSARLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVYG
AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASSGNNDFRARGRGWLGSLETGLPFS
ITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWW
VQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLVLQAGLEARVRENITLGVQAGYAHSINGSSAESYNSQATLNVT
F

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66200.1 hypothetical protein Virulence PAI III 536 Protein 0.0 95
SF2991 NP_708765.1 outer membrane fluffing protein Not tested SHI-1 Protein 0.0 93
sap AAK00474.1 Sap Not tested SHI-1 Protein 0.0 93
aidA ADD91708.1 AidA Not tested PAI-I AL862 Protein 0.0 88
flu CAI43838.1 antigen 43 Not tested LEE Protein 0.0 88
Z1211 NP_286746.1 adhesin Not tested TAI Protein 0.0 58
Z1651 NP_287154.1 adhesin Not tested TAI Protein 0.0 58
unnamed AAL08472.1 putative autotransporter Not tested SRL Protein 0.0 56
unnamed CAE85197.1 antigen43 protein orthologue Not tested PAI V 536 Protein 0.0 56

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
flu YP_002414067.1 antigen 43 (Ag43) phase-variable biofilm formation autotransporter; CP4-44 prophage VFG1675 Protein 0.0 95
flu YP_002414067.1 antigen 43 (Ag43) phase-variable biofilm formation autotransporter; CP4-44 prophage VFG0655 Protein 0.0 93
flu YP_002414067.1 antigen 43 (Ag43) phase-variable biofilm formation autotransporter; CP4-44 prophage VFG1063 Protein 0.0 56