Gene Information

Name : flu (ECED1_5177)
Accession : YP_002400911.1
Strain : Escherichia coli ED1a
Genome accession: NC_011745
Putative virulence/resistance : Virulence
Product : antigen 43 (Ag43) phase-variable biofilm formation autotransporter; CP4-44 prophage
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG3468
EC number : -
Position : 5107795 - 5110923 bp
Length : 3129 bp
Strand : +
Note : Evidence 1b : Function experimentally demonstrated in the studied species; PubMedId : 20392470, 97257509, 99212225, 2661530, 9298646; Product type h : extrachromosomal origin

DNA sequence :
ATGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATTACGGGCGCTTTCGTGGTTGTCTCCGA
ACTGGCCCGCACACGGGGTAAACGTGGCGGTGTGGCGGTTGCACTGTCTCTTGCCGCGGTCACGTCACTCCCGGTGCTGT
CTGCTGACATCGTTGTGCACCCGGGTGAAACAGTGAATGGCGGAACACTGGTAAACCATGACAACCAGTTTGTATCCGGA
ACAGCTAATGGCGTGACTGTCAGTACCGGGCTTGAGCTGGGGCCGGACAGTGACGAAAACACCGGCGGGCAATGGATAAA
AGCGGGTGGCACAGGCAGAAACACCACTGTCACCGCAAATGGTCGTCAGATTGTGCAGGCAGGAGGAACTGCCAGTGATA
CGGTTATTCGTGATGGCGGAGGGCAGAGCCTTAACGGACTGGCGGTGAACACCACGCTGGATAACAGAGGTGAGCAGTGG
GTACACGGGGAAGGGAAAGCTACCGGTACAATTATTAACCAGGATGGTTACCAGACCATAAAACATGGCGGACTGGCAAC
CGGAACCATCGTCAACACCGGTGCAGAAGGTGGTCCGGAGTCTGAAAATGTGTCCACTGGTCAGATGGTCGGGGGGACTG
CTGAATCCACCACCATCAACAACAATGGCCGACAGGTTATCTGGTCTTCGGGGGTATCCCGGGACACTCTCATTTACACA
GGCGGTGACCAGACGGTACACGGAGAGGCACATAATACCCGGCTGGAGGGAGGAAACCAGTATGTGCACAAATACGGACT
TGCGCTGAACACGGTAATTAACGAGGGGGGCTGGCAGGTTGTTAAGGCAGGTGGTACTGCCGGTAACACCACCATAAATC
AGAACGGCGAGCTGAAGGTTCATGCCGGCGGAGAAGCGTCTGATGTCACCCAGAACACGGGCGGTGCACTGGTTACCAGT
ACAGCTGCAACCGTCACCGGCACAAACCGCCTGGGAGCATTCTCCGTTGTGGCGGGTAAGGCTGATAATGTCGTACTGGA
AAATGGCGGACGTCTGGATGTGCTGAGCGGACACACAGCCACTAATACCCGTGTGGATGATGGCGGAACGCTGGATGTCC
GCAACGGTGGTGCCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGGGCCGCTGTCAGTGGT
ACCCGGAGCGACGGAACGGCATTCCGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCAC
GCTGAACGCCGGTGATACGGCCACGGATACCACGGTAAATGGCGGACTACTCACTGCCAGAGGGGGCTCGCTGGCGGGCA
CCACCACGCTGAATAACGGTGCCACGCTTACCCTTTCCGGGAAAACGGTGAATAACGATACCCTGACCATCCGTGAAGGT
GATGCACTCCTGCAGGGAGGCACTCTTACCGGTAACGGCAGGGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAA
CACCACACTCACCCAGAAAACCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCA
TTGCTCAGCGCGGTACAACCCTGAAGCTGACCGGCAGCACAGTGCTGAACGGTGCCATTGACCCCACGAATGTCACCCTG
ACATCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTTCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGAT
TCATTTCACCTCCGCCCGCACAGGGAAGTTCGTTCCGACAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCA
TCAGCCTGCGTGTACGCCCGGATATGGCGCAGAACAATGCTGACAGACTGGTCATTGACGGTGGCAGGGCAACCGGAAAA
ACCATCCTGAATCTGGTGAACGCCGGCAACAGTGGTACGGGGCTGGCGACCACCGGTAAGGGTATTCAGGTGGTTGAGGC
CATTAACGGTGCCACCACGGAGGAAGGGGCTTTTGTCCAGGGGAATATGCTGCAGGCCGGGGCCTTTAACTACACCCTCA
ACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAGAACGTTATCGTGCTGAAGTCCCCCTGTACGCCTCCATGCTG
ACACAGGCAATGGACTATGACCGGATTCTGGCAGGCTCACGCAGCCATCAGACCGGTGTAAACGGTGAAAATAACAGCGT
CCGTCTCAGCATTCAGGGCGGCCATCTCGGTCACGATAACAACGGCGGTATTGCCCGTGGGGCCACGCCGGAAAGCAGCG
GCAGCTATGGCTTCGTCCGTCTGGAGGGTGACCTGCTCAGAACAGAGGTCGCCGGTATGTCACTGACGACAGGAGTGTAT
GGTGCTGCAGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGCGCCGGTACGGTCCGGGATGATGCCGGCAG
CCTGGGTGGATACCTGAATCTGACACATACATCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCA
TGAAAGCATCATCGGACAATAACGACTTCCGCGCCCGGGGCCGGGGCTGGCTGGGTTCACTGGAAACCGGTCTGCCCTTC
AGTATCACTGACAACCTGATGCTGGAGCCACGACTGCAGTACACCTGGCAGGGGCTCTCCCTGGATGACGGTAAGGACAA
CGCCGGTTATGTGAAGTTCGGGCATGGCAGTGCACAACATGTGCGTGCCGGTTTCCGCCTGGGCAGCCACAACGATATGA
CCTTTGGTGAAGGCACCTCATCCCGTGACACCCTGCGCGACAGTGCAAAACACAGTGTGCGTGAACTGCCGGTGAACTGG
TGGGTACAGCCTTCTGTTATCCGCACCTTCAGTTCCCGGGGGGACATGAGCATGGGTACAGCCGCAGCCGGCAGTAACAT
GACGTTCTCACCGTCACAGAATGGTACGTCACTGGACCTGCAGGCCGGACTGGAAGCCCGTGTCCGGGAAAACATCACCC
TGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACGCTGAATGTG
ACCTTCTGA

Protein sequence :
MLMKRHLNTCYRLVWNHITGAFVVVSELARTRGKRGGVAVALSLAAVTSLPVLSADIVVHPGETVNGGTLVNHDNQFVSG
TANGVTVSTGLELGPDSDENTGGQWIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQW
VHGEGKATGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSTGQMVGGTAESTTINNNGRQVIWSSGVSRDTLIYT
GGDQTVHGEAHNTRLEGGNQYVHKYGLALNTVINEGGWQVVKAGGTAGNTTINQNGELKVHAGGEASDVTQNTGGALVTS
TAATVTGTNRLGAFSVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADSGAAVSG
TRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLLTARGGSLAGTTTLNNGATLTLSGKTVNNDTLTIREG
DALLQGGTLTGNGRVEKSGSGTLTVSNTTLTQKTVNLNEGTLTLNDSTVTTDVIAQRGTTLKLTGSTVLNGAIDPTNVTL
TSGATWNIPDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGK
TILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNMLQAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASML
TQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVY
GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLTHTSSGLWADIVAQGTRHSMKASSDNNDFRARGRGWLGSLETGLPF
SITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSAKHSVRELPVNW
WVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAEGYNSQATLNV
TF

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66200.1 hypothetical protein Virulence PAI III 536 Protein 0.0 97
SF2991 NP_708765.1 outer membrane fluffing protein Not tested SHI-1 Protein 0.0 94
sap AAK00474.1 Sap Not tested SHI-1 Protein 0.0 94
aidA ADD91708.1 AidA Not tested PAI-I AL862 Protein 0.0 87
flu CAI43838.1 antigen 43 Not tested LEE Protein 0.0 87
unnamed AAL08472.1 putative autotransporter Not tested SRL Protein 0.0 59
Z1211 NP_286746.1 adhesin Not tested TAI Protein 0.0 59
Z1651 NP_287154.1 adhesin Not tested TAI Protein 0.0 59
unnamed CAE85197.1 antigen43 protein orthologue Not tested PAI V 536 Protein 0.0 58

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
flu YP_002400911.1 antigen 43 (Ag43) phase-variable biofilm formation autotransporter; CP4-44 prophage VFG1675 Protein 0.0 97
flu YP_002400911.1 antigen 43 (Ag43) phase-variable biofilm formation autotransporter; CP4-44 prophage VFG0655 Protein 0.0 94
flu YP_002400911.1 antigen 43 (Ag43) phase-variable biofilm formation autotransporter; CP4-44 prophage VFG1063 Protein 0.0 59