Gene Information

Name : lysS (PCC7424_0345)
Accession : YP_002375681.1
Strain : Cyanothece sp. PCC 7424
Genome accession: NC_011729
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 381699 - 383420 bp
Length : 1722 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCATCTAATCAATCCCAACCCCAACCCCAATCCGGCTCTTCACTCGAAGAAATTCGCGCTACACGCCTTGAGAAAGT
TGAACAACTCAAACAACTGGGATTGAATCCTTATGAGTATAAATGGGAATCAACCCACCATGCAGCCGAATTACAGGAAA
AATACGCGGATTTAGAAAATGGCGAAGAAATAGACTTAGAAGTATCGATCGCTGGTCGTATTATAGCCCGTCGGGTGTTT
GGTAAATTAGCCTTTTTTAACCTCCAAGATGAAACCGGAACCATACAGTTATATTTAGAGAAAAAGAGAATTAGTCAAAC
GATGGCAGATGTTGCCAATGCCTTTAATACTGTCCTCAAATTAACCGATACGGGGGATATTTTAGGAGCAAAAGGAACAA
TTAAACGGACAGAAAAAGGGGAACTCTCCATTTATGTGACTGAGTATGCTATTCTGACTAAAACTTTATTACCCTTACCC
GATAAATTTCACGGATTAACGGATATAGAAAAACGCTATCGTCAGCGTTATGTAGATTTAATTATTAATCCGGAAGTGCG
AGAAACCTTTCGTCGTCGCGCCCAAATTACGGCATCCATTCGTCGTTATTTAGAACAAGAAGGATTTTTAGAAATTGAAA
CCCCAGTCTTACAAAGTGAAGCCGGGGGGGCAGATGCTCGTCCTTTTATTACCTATCACAATACCTTAGAGATGGATTTA
TATCTCCGCATTGCCACCGAATTACATTTAAAACGGTTAATTGTGGGCGGGTTTGAAAAAGTCTTTGAGATGGGCAGAAT
TTTTAGGAATGAAGGGGTTTCTACTCGTCACAATCCCGAATTTACCTCGATCGAAATCTATCAGGCTTATGCAGATTATC
ATGATATGATGGTCTTAACGGAAAACATTATCACCACAGCCGCTAAAGATGTTGTCGGGACTTTAAAGATTACTTATCAA
GAGGAAGAAATAGATTTAACACCTCCTTGGCGACGGGTAACTATGCACGAATTAGTCGAGGAAAAAACCGGCTTAGATTT
GAATAAATTTAAAACCTTTGAGGAAGCAAGAACCGCAGCGAAAAAAGCCGGGATAGACGTTCCTGAAGACTGTAAAACTC
TCGGAAAATTGCTCAATGAAGCCTTTGAACAAAAAGTAGAAGAAAGTTTAATTCAGCCCACTTTTGTGTTAGATTTTCCC
GTAGAAATTTCCCCCCTAGCTAAACCTCATCGCTCTAAATCTGGGTTAGTAGAACGATTTGAATTATATATTGTCGGACG
GGAATTAGCGAATAGTTTTTCTGAGTTAACCGATCCGATCGATCAACGGAAAAGATTAGAAGCACAAGCAGAGAAAAAAG
CAGCCGGAGATTTAGAGGCGCATGGAGTTGATGAAGATTTCTTAACCGCTTTAGAGTATGGAATGCCTCCTACCGGTGGG
TTAGGAATAGGGATCGATCGCTTGATTATGTTGTTGACAAATTCTCCGAGTATTCGAGATGTGATTGCTTTCCCTTTACT
GAAGAGTCAAAGTGCAGCGTTAAAAGGATTTGATTATGATCCAGATAAACAAATTTTGAGAATTGAGTTTATTAATGGCA
GTGTTTATAAGTATCTTGATGTTCCTAAATCTATTTATCAGGAATTAGAAAAAGCTCCCTCAAAAGGTCAGTTTTTTAAC
GCTCAAATTCGAGATAAGTTTGGGTTCGATCGGGAAATTTAA

Protein sequence :
MSSNQSQPQPQSGSSLEEIRATRLEKVEQLKQLGLNPYEYKWESTHHAAELQEKYADLENGEEIDLEVSIAGRIIARRVF
GKLAFFNLQDETGTIQLYLEKKRISQTMADVANAFNTVLKLTDTGDILGAKGTIKRTEKGELSIYVTEYAILTKTLLPLP
DKFHGLTDIEKRYRQRYVDLIINPEVRETFRRRAQITASIRRYLEQEGFLEIETPVLQSEAGGADARPFITYHNTLEMDL
YLRIATELHLKRLIVGGFEKVFEMGRIFRNEGVSTRHNPEFTSIEIYQAYADYHDMMVLTENIITTAAKDVVGTLKITYQ
EEEIDLTPPWRRVTMHELVEEKTGLDLNKFKTFEEARTAAKKAGIDVPEDCKTLGKLLNEAFEQKVEESLIQPTFVLDFP
VEISPLAKPHRSKSGLVERFELYIVGRELANSFSELTDPIDQRKRLEAQAEKKAAGDLEAHGVDEDFLTALEYGMPPTGG
LGIGIDRLIMLLTNSPSIRDVIAFPLLKSQSAALKGFDYDPDKQILRIEFINGSVYKYLDVPKSIYQELEKAPSKGQFFN
AQIRDKFGFDREI

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-106 49
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 1e-85 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_002375681.1 lysyl-tRNA synthetase VFG1668 Protein 7e-107 49