Gene Information

Name : Msil_3318 (Msil_3318)
Accession : YP_002363586.1
Strain : Methylocella silvestris BL2
Genome accession: NC_011666
Putative virulence/resistance : Virulence
Product : L-lysine 6-monooxygenase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG3486
EC number : 1.14.13.59
Position : 3643062 - 3644387 bp
Length : 1326 bp
Strand : -
Note : KEGG: oan:Oant_3254 L-lysine 6-monooxygenase (NADPH)

DNA sequence :
GTGACGACGGAACAGACGCTAGATCTTGCCGGCATCGGGGTTGGTCCTTTCAATCTGAGTTTGGCGGCTCATCTCGATTC
CATCCCCGAGCTCGACGTCCGCTTTTTCGAGCAGCGAGACAGGCTCGATTGGCATCCAGGCTTGATGTTGCCAAACGCCG
ATTTGCAAACGTCCTTCCTCAAGGATCTCGTCACGGCGACCCATCCGACAAGCCCCTGGTCGTTCCTCGCCTATCTTGTC
GCCCAGAAGCGCTTTCTCAAATTCCTCAACGCCGAATTCGAGGCGACGCCGCGAAGGGAATTCGCGAATTATCTCGAATG
GGCGGCGAATGGATTGCCGAATCTGCAATTCAACACGCGCGTCAGGGAAATCTCATTCGACCGATCGGCGTTCAAGGTGC
GGTTTGACGAAGACTCCTGCAGCGCTCAACATATCGCGCTGGGCATAGGCCTATCGCCCTTCCTGCCGGCATGGGCGGAT
GAGCAGATCGGCGACGATTGCTTTCATTCGTCAACGACCACGCGCCGTCTCGACGGCATGACAGGCGAGCGCATCGTGGT
GGTTGGCGGAGGCCAGAGCGGCGCCGAAATCATGCTTCACCTGCTATCCGAAGGCCGACATACGCCGGCTGAAATCAGAT
GGGTGAGCAGACGACCCAATTTCGAGCCGCTCGACGCAACGCCCTTTACCAACGAGCTGTTCACTCCCGAATATGTGGAC
AGCTTCCGTCGCCTGGGCGAGGATCGACGGCACGCTCTCGTCGCCTCGCAGAAACTCGCCGCCGACGGCGCGTCGGCTTC
GACTTTGCGAGCGCTCTACCGGCGCATATACGCGATGAAATATCTGGAGGACAATGATGTCGATATCGGCCTTCTGCCGC
ATCGCGACGTGATCGAGGTCGATCGGCAGGGCAAGGAATTCAAGCTCGTCATGCGCAACGGATTCGATGGCGGCATTGAA
GTCATCACAGCGCGGACAATCATTCTCGCCACCGGATACACATTCAAATTTCCAGAGTTTCTGGCGCCGCTCAAGGATCG
GATCAGCATCGATCGCCACGGCAATTTTATTCTTGGCGACGATTTCTCGGTGAAGTGGGATGGGCCGCGGCGTCATCACA
TCTTTGCGCTCAATGCCGGAAGGTCAAGCTATGGCGTCGCCGAGCCGCAGCTCAGCCTGACAGCCTGGCGCAGCGCCGTC
ATCGTCAATGCGCTTCTCCGCAAGCCTCATTTCGATTTGTCTCTGCCACCATCCCTCATGCGCTGGTCGACAGGCGACGA
TGTCTATGTAAAATCAGCTGGCGGAGCCGTATCGATCGCGGGCTGA

Protein sequence :
MTTEQTLDLAGIGVGPFNLSLAAHLDSIPELDVRFFEQRDRLDWHPGLMLPNADLQTSFLKDLVTATHPTSPWSFLAYLV
AQKRFLKFLNAEFEATPRREFANYLEWAANGLPNLQFNTRVREISFDRSAFKVRFDEDSCSAQHIALGIGLSPFLPAWAD
EQIGDDCFHSSTTTRRLDGMTGERIVVVGGGQSGAEIMLHLLSEGRHTPAEIRWVSRRPNFEPLDATPFTNELFTPEYVD
SFRRLGEDRRHALVASQKLAADGASASTLRALYRRIYAMKYLEDNDVDIGLLPHRDVIEVDRQGKEFKLVMRNGFDGGIE
VITARTIILATGYTFKFPEFLAPLKDRISIDRHGNFILGDDFSVKWDGPRRHHIFALNAGRSSYGVAEPQLSLTAWRSAV
IVNALLRKPHFDLSLPPSLMRWSTGDDVYVKSAGGAVSIAG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
iucD YP_002414043.1 L-lysine 6-monooxygenase Virulence Not named Protein 2e-88 43
iucD NP_839220.1 lysine:N6-hydroxylase Virulence SHI-2 Protein 1e-88 43
iucD NP_709457.1 lysine:N6-hydroxylase Virulence SHI-2 Protein 1e-88 43
iucD AAK71634.1 IucD Virulence SHI-3 Protein 4e-88 43
iucD AAD44749.1 IucD Virulence SHI-2 Protein 1e-88 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Msil_3318 YP_002363586.1 L-lysine 6-monooxygenase VFG0937 Protein 9e-89 43
Msil_3318 YP_002363586.1 L-lysine 6-monooxygenase VFG1084 Protein 3e-88 43
Msil_3318 YP_002363586.1 L-lysine 6-monooxygenase VFG0618 Protein 6e-89 43