Gene Information

Name : fliI (Msil_1657)
Accession : YP_002361967.1
Strain : Methylocella silvestris BL2
Genome accession: NC_011666
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 1792559 - 1793908 bp
Length : 1350 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGATCGATCGCCCCGCTGACGGAGCGCTCGCGAAACTGGAGCGGGCCATGACGCAGGCGGCGGCGGAGCCGCTGGTGCG
CGTCAGCGGCGCCGTCACCGAATTATCGCCCTCGCATGCCCGCGTCGCCGGACTATCATCCTTTGTGAAGCTCGGCGAAT
GCGTGCGCTTTGGTCCCGAAGCCGAAGGCGCGCGCAATCGCGGCGAGGTGGTGCGGATCGACGCGCAAGGCGTCATCGTC
AAGACCTTCGAGGCGGGCGCATTGACGAGCCTCGGCGAGCGCGTGTTTCGCGCGGGACCGCTGCGCATCGCGCCGCATCC
GGGCTGGAAGGGCCGCGTCGTCAACGCTTTCGGCGCGCCGATCGACGGCGCCGGGCCGTTGCCGCAGGGAGATCGCAGCG
CCATGATCGACGCCCCACCGCCGAGCGCCATGCGACGCGCGCGCGTCGCATGGCCGCTGCGCACGGGCGTCCGCGTCATC
GACATCTTCACCCCGCTTTGCGCTGGTCAGCGCATCGGCATATTCGCCGGCTCCGGCGTCGGTAAATCCACCTTGCTGGC
GATGCTCGCCGGCTCCAAGGATTTCGATACCGTCGTCGTCGCGCTGGTCGGCGAACGCGGCCGCGAGGTGCGCGAATTCC
TCGATGACGCGCTTGCCGCCAATCGCCCGATCTCGGTCGCCGTCGTCGCGACGGGAGACGAAACGCCGTCGATGCGCCGG
CTCGCGCCGCGCACGGCGATGGCGATCGCCGAGTTCTTTCGCGATCGCGGCGACCGCGTGCTGCTGATTATCGATTCCGT
CACCCGTTTCGCCCATGCGGCGCGCGACGTCGCTCTGGCGGCCGGCGAGCCCCCCGTCGCGCGCGGCTACACGCCGAGCG
TTTTCAGCGATTTGCCGAAACTGCTCGAGCGCGCGGGGCCCGGCGAAGAAGGCGGAGGGTCGATCACTGGCGTCTTCTCC
GTGCTCGTCGACGGCGACGACCACAATGATCCCGTGGCGGACGCCGTGCGCGGCACGCTCGACGGGCATATCGTGCTGGA
TCGGGCGATCGCCGATCAGGGCCGCTTTCCCGCCGTTAATGTGCTCGCCTCGATCTCGCGGCTGGCGCAGATCGCCTGGA
CGGCCGAACAGCGCGATCTTGTCGTCAAGCTGCGCGGCATGATCGCGCGCTTCGAGGACAGCAGCGATCTGCGCCTGATG
GGCGGCTACAAGGCGGGGCAGGACGCAGATCTCGATCAGGCGGTCGCGCTTGCGCCAAAAATCTACAAGGCGCTGCAACA
ATCGCCGCAATCAACGCCAAGCGCCGACGCCTTTCGCGAACTCGCACAGAGTCTCACAGGCGCGTCCTGA

Protein sequence :
MIDRPADGALAKLERAMTQAAAEPLVRVSGAVTELSPSHARVAGLSSFVKLGECVRFGPEAEGARNRGEVVRIDAQGVIV
KTFEAGALTSLGERVFRAGPLRIAPHPGWKGRVVNAFGAPIDGAGPLPQGDRSAMIDAPPPSAMRRARVAWPLRTGVRVI
DIFTPLCAGQRIGIFAGSGVGKSTLLAMLAGSKDFDTVVVALVGERGREVREFLDDALAANRPISVAVVATGDETPSMRR
LAPRTAMAIAEFFRDRGDRVLLIIDSVTRFAHAARDVALAAGEPPVARGYTPSVFSDLPKLLERAGPGEEGGGSITGVFS
VLVDGDDHNDPVADAVRGTLDGHIVLDRAIADQGRFPAVNVLASISRLAQIAWTAEQRDLVVKLRGMIARFEDSSDLRLM
GGYKAGQDADLDQAVALAPKIYKALQQSPQSTPSADAFRELAQSLTGAS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 9e-59 47
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-59 47
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 4e-59 47
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-59 47
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 7e-59 47
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-62 44
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-62 44
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-62 44
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-54 44
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-54 44
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 1e-61 43
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-62 43
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-61 43
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 9e-49 43
escN YP_003223475.1 translocator EscN Virulence LEE Protein 5e-52 43
escN CAI43874.1 EscN protein Virulence LEE Protein 4e-52 43
escN YP_003236088.1 translocator EscN Virulence LEE Protein 8e-53 43
escN AAC38383.1 EscN Virulence LEE Protein 2e-52 43
escN AAC31513.1 L0034 Virulence LEE Protein 9e-53 43
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 9e-53 43
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-51 43
escN CAC81862.1 EscN protein Virulence LEE II Protein 7e-52 43
escN AAK26715.1 EscN Virulence LEE Protein 6e-52 43
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-52 43
escN AAL57542.1 EscN Virulence LEE Protein 6e-52 43
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-52 43
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 2e-65 42
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-61 42
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-61 42
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-61 42
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 1e-49 42
unnamed AAL06369.1 EscN Virulence LEE Protein 3e-51 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_002361967.1 flagellum-specific ATP synthase VFG0515 Protein 4e-59 47
fliI YP_002361967.1 flagellum-specific ATP synthase VFG0048 Protein 5e-61 44
fliI YP_002361967.1 flagellum-specific ATP synthase VFG0730 Protein 1e-52 43
fliI YP_002361967.1 flagellum-specific ATP synthase VFG0812 Protein 5e-53 43