Gene Information

Name : lysS (Aflv_0073)
Accession : YP_002314442.1
Strain : Anoxybacillus flavithermus WK1
Genome accession: NC_011567
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 82006 - 83490 bp
Length : 1485 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCGCATGAGGAATTAAATGATCAGCTATTAGTTCGTCGTGAAAAGTTACATAAGTTGCGTGAAAAAGGGATCGATCC
ATTTGGAAAACGATTCGAGCGTACACATCAAGCAGAAGAATTGTTTACATTATACGGTCATTTATCAAAAGAAGAGTTGG
AAGAAAAGCAAATTCATGTTTCTATCGCTGGTCGCATTATGACGAAGCGCGGGAAAGGGAAAGCGGGATTTGCTCATATT
CAAGATTTGACGGGACAAATTCAAATTTATGTGCGCAAAGACGATGTTGGTGAAGAGCAGTATGAAATTTTCGATACGTC
AGATTTAGGAGATATTGTCGGTGTACAAGGAACAGTATTCAAAACAAAAGTGGGAGAATTGTCTATCAAGGTACACTCGT
ATGAAATGTTGACGAAATCGTTGCGCCCGCTCCCTGAGAAATATCACGGGCTCAAAGATATCGAGCAACGCTACCGCCAA
CGCTACCTCGATTTAATTATGAATCCAGAAAGTAAAAAAACGTTTATTACACGCAGTTTAATTATTCAGTCGATGCGTCG
CTATTTAGATCAAAAAGGATATTTAGAAGTAGAGACGCCGATGATGCACTCAATTGCCGGAGGAGCTGCAGCCCGTCCAT
TTATTACGCACCATAACGCTCTAGATATGACATTGTATATGCGTATTGCAATTGAATTACATTTAAAACGTTTAATTGTA
GGTGGATTAGAGAAAGTGTACGAAATCGGCCGTGTATTCCGAAACGAAGGCATTTCAACACGTCACAATCCAGAGTTTAC
GATGCTTGAGCTTTACGAGGCTTATGCTGATTACACTGATATTATGAGATTAACAGAAGATATGATTGCACATATTGCTC
AAGAAGTGCTTGGAACGACAAAAATCCAATATGGAGAACATGAAGTAGATTTAACACCGTCATGGAAACGACTTCATATG
GTCGATGCGATTAAAGATTATGTGGGTGTTGACTTCTGGAAGCACATGAGCGATGAAGAAGCGCGTCAATTGGCGAAAGA
GCATGGAGTAGAAGTAGCTCCACATATGACATTCGGTCATATCGTAAATGAATTTTTCGAACAAAAAGTAGAACAACATC
TTATTCAGCCAACATTTATTTATGGGCACCCAGTTGAGATTTCGCCTTTAGCGAAAAAAAATCCAGAAGATCCTCGTTTC
ACAGATCGATTTGAACTATTTATCGTTGCACGTGAGCATGCAAATGCATTCACAGAATTAAATGACCCAATCGATCAACG
TGAACGATTTGAAGCGCAGTTAAGAGAAAAAGAACAAGGAAACGACGAAGCGCATGAGATGGACGAAGATTTTATCGAAG
CATTAGAATACGGGATGCCACCAACGGGTGGGTTAGGAATTGGCATTGATCGTCTTGTTATGTTGTTAACGAACTCTCCA
TCGATCCGCGATGTATTGTTATTCCCTCAAATGCGTCATAAATAA

Protein sequence :
MSHEELNDQLLVRREKLHKLREKGIDPFGKRFERTHQAEELFTLYGHLSKEELEEKQIHVSIAGRIMTKRGKGKAGFAHI
QDLTGQIQIYVRKDDVGEEQYEIFDTSDLGDIVGVQGTVFKTKVGELSIKVHSYEMLTKSLRPLPEKYHGLKDIEQRYRQ
RYLDLIMNPESKKTFITRSLIIQSMRRYLDQKGYLEVETPMMHSIAGGAAARPFITHHNALDMTLYMRIAIELHLKRLIV
GGLEKVYEIGRVFRNEGISTRHNPEFTMLELYEAYADYTDIMRLTEDMIAHIAQEVLGTTKIQYGEHEVDLTPSWKRLHM
VDAIKDYVGVDFWKHMSDEEARQLAKEHGVEVAPHMTFGHIVNEFFEQKVEQHLIQPTFIYGHPVEISPLAKKNPEDPRF
TDRFELFIVAREHANAFTELNDPIDQRERFEAQLREKEQGNDEAHEMDEDFIEALEYGMPPTGGLGIGIDRLVMLLTNSP
SIRDVLLFPQMRHK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 3e-126 53

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_002314442.1 lysyl-tRNA synthetase VFG1668 Protein 1e-126 53