Gene Information

Name : lysS (Rleg2_3420)
Accession : YP_002282913.1
Strain : Rhizobium leguminosarum WSM2304
Genome accession: NC_011369
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 3536620 - 3538113 bp
Length : 1494 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGAACGACAAGACCGAAAACACCCTTTCCTCCGACGTGACTGAGGTGCGCGCCCAGAAGCTGAAGCTGCTGCGCGAGCA
GGTCGGCGACGTCTATCCGGCGCATTTCCACCGGACGCTGACCAATGCCGAGCTCGGCGCCAAGTACGAATCTCTGGAAT
CCGACGTCGAGACGCAGGATGTCGTCACCGTCGCCGGCCGCGTCTATTCCTCGCGCAACTCCGGCATGTTCATGGATATC
CATGACGCTTCCGGCAAGATCCAGATCTTCAGCCACAAGGACACGACGCCGGAAGAGGCCCGCGCTTTGCTGCCGATGAT
CGACATCGGCGACATCATCGGCGTGACCGGCATCGTGCGCCGCACCAAGCGCGGCGAGCTGACGATCAACGCCCAGAAGA
TCGAGATGCTGACCAAGTCGCTGCTGCCGATGCCCGAGAAGTGGCACGGCCTCTCCGACATCGAGCTGCGGTATCGCAAG
CGCCATCTCGACATCATGACCAACGAGGATTCGAAGCTTCGCTTCCAGCAGCGCTCGAAGATCGTCTCCGGCATCCGCCG
CTTCATGGAGAATGACGGCTTCATGGAAGTCGAGACGCCGATGCTGCAATCGATCTATGGCGGCGCCACCGCCGAGCCGT
TCAAGACGCATCACAACACGCTGAAGCTCGACATGTATCTGCGCATCGCGCCGGAACTGTTCCTGAAGCGCACGCTGGTA
TCGGGCCTCACCGACAAGGTCTTCGAGATCAACCGGAACTTCCGCAACGAAGGCGTCTCCACCCGGCACAATCCCGAGTT
CACGATGATGGAGTGTTACTGGGCCTATGCCGATTACGAGGACATGATGGACCTCGTCGAGCGGCTGTTCGAAACCCTGG
CGCTCTCCATCCATGGCACCACCGAATTCGATTTCGGCGACAAGCAGCTGTCCTTCAAGGGTCCGTTCAAGCGCGTACCG
ATGCCTGACGCCGTCAAGCAAGTGACCGGCATCGATTTCCTTGCGATCAAGACCGACGAGGAAGCACGCACTGCTGCCAA
GGCTGCCGGCTTCGCGGTCGAGAAGGACTGGACCTGGGGCGAATGCCTTGCCTTCATCTTCGAGGAAAAGGTTGAATCCA
CACTGATCCAGCCGTCTCACGTCACGCATTTCCCGAAGGACATCTCACCCTTCGCCAAGGAAGTGCCGGGCGAACCGCGC
CTCGTCGAGCGCTTCGAGACCTATTGCAACGCCTGGGAACTCGGCAACGCCTTCTCCGAGCTCAACGATCCCGAAGAGCA
GCGCCGCCGCATGGTCGAGCAGCTCGAACAGGCGCATGCCCGCGGCGAAAAGGAAAAGCAGTTGGACGAGGAATTCCTCG
ACGCGATCGACCAGGGCATGCCGCCGGCCGGCGGCCTCGGCATCGGCGTCGACCGCCTGATCATGCTTTTGACCAACGCC
CCGTCGATCCGCGACGTCATCCTCTTCCCGGCCCGCCGCAACAAGGCCGACTGA

Protein sequence :
MNDKTENTLSSDVTEVRAQKLKLLREQVGDVYPAHFHRTLTNAELGAKYESLESDVETQDVVTVAGRVYSSRNSGMFMDI
HDASGKIQIFSHKDTTPEEARALLPMIDIGDIIGVTGIVRRTKRGELTINAQKIEMLTKSLLPMPEKWHGLSDIELRYRK
RHLDIMTNEDSKLRFQQRSKIVSGIRRFMENDGFMEVETPMLQSIYGGATAEPFKTHHNTLKLDMYLRIAPELFLKRTLV
SGLTDKVFEINRNFRNEGVSTRHNPEFTMMECYWAYADYEDMMDLVERLFETLALSIHGTTEFDFGDKQLSFKGPFKRVP
MPDAVKQVTGIDFLAIKTDEEARTAAKAAGFAVEKDWTWGECLAFIFEEKVESTLIQPSHVTHFPKDISPFAKEVPGEPR
LVERFETYCNAWELGNAFSELNDPEEQRRRMVEQLEQAHARGEKEKQLDEEFLDAIDQGMPPAGGLGIGVDRLIMLLTNA
PSIRDVILFPARRNKAD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 3e-98 48

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_002282913.1 lysyl-tRNA synthetase VFG1668 Protein 2e-98 48