Gene Information

Name : Rleg2_1232 (Rleg2_1232)
Accession : YP_002280752.1
Strain : Rhizobium leguminosarum WSM2304
Genome accession: NC_011369
Putative virulence/resistance : Resistance
Product : Bcr/CflA subfamily drug resistance transporter
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG2814
EC number : -
Position : 1245396 - 1246586 bp
Length : 1191 bp
Strand : -
Note : TIGRFAM: drug resistance transporter Bcr/CflA subfamily; PFAM: major facilitator superfamily MFS_1; KEGG: ret:RHE_CH01569 florfenicol/chloramphenicol resistance transporter, Bcr/CflA subfamily

DNA sequence :
ATGTCTTATCCGAAAATTCCGTCCTGGACGTATTCCGTGCCGGCGACGTTGTTGCTGATGGCGCCTTTCGACATCCTGGC
CTCGCTTGCAATGGACATCTATCTGCCGGTTGTGCCGACCATGCCGCAGGCGCTTGGCACCTCGCCTGCCGTCATTCAAT
TGACCCTTAGCCTTTACATGCTTGTGCTTGGCGTCGGGCAGATCGTCTTTGGACCGATCTCCGATATCGTTGGGCGGCGG
CCCGTTCTGCTCGGCGGGGCGGCACTCTTTGCCGTTTCCTCCTTCCTACTTGCCGGAACCTCATCGGCCTCTCTCTTCAT
GGCCCTGCGATTGCTGCAGGCCATCGGCGCCTCGGCGGCGCTGGTCGCCACTTTCGCAACGGTGCGTGACGTTTATGCCG
AACGCCCGGAAAGCAGCGTGATCTATAGCCTGCTTGGTTCCATGCTGTCCTTTGTGCCCGCATTCGGGCCGATCGTTGGA
GCGGTGATCGCGGATCGGTACGGGTGGCGAGCCATCTTCGTCATCATCGGCGTGCTTTCGGTCGTCGCAATTCTCAACGC
CGGCATGCGGTGGCATGAAACCCGCCCTGCCGCCGCCACGGCGACCATCTCGTTCCGGCCGATTCTGACCAGCTTTCCGT
TCTGGGTCTACACAAGCGGCTTCAGCGCGGCGATGGGAGCCTTCTTCGTCTTCTTCTCGACGGCACCTCGCATCCTGATC
GATCAATCCGGTTTTTCCGGCATCACCTTCAGCTTCATCTTTGCCTCGGTGGCTTTGGTGATGATCGTGACGGCGCGCTT
TGCCAAAGGCTTCGTCACGCGCTGGGGTATCGCAGGCAGCCTTGCCAGAGGCATGGCGATGTTGCTTCTCGGCGCCGTGA
TGCTTGCCGCGGGAGAGATGTTTCTGCAGGCACCCCTCATCGGCTTCGTCCTGCCGATGTGGATCATCGCGATCGGCATC
GTCTTTGCGACGGCGGTCACCGCCAATGGTGCACTCAAGGCGTTCGACGATACCGCCGGAACGGCGGTGGCGCTCTATTT
CTGCATCGAAAGCCTGCTGGTCGGGGTCGCAGGCACCTTGTTCGTCGTATTGCTCGACGGCGACACCGCCTGGCCGCTGG
CAGCTTATGCCGCCGTGACGGCGCTGATCACCCTTGCCGGATTGCGGCGGCTTTCGTGCAAGACAGCCTGA

Protein sequence :
MSYPKIPSWTYSVPATLLLMAPFDILASLAMDIYLPVVPTMPQALGTSPAVIQLTLSLYMLVLGVGQIVFGPISDIVGRR
PVLLGGAALFAVSSFLLAGTSSASLFMALRLLQAIGASAALVATFATVRDVYAERPESSVIYSLLGSMLSFVPAFGPIVG
AVIADRYGWRAIFVIIGVLSVVAILNAGMRWHETRPAAATATISFRPILTSFPFWVYTSGFSAAMGAFFVFFSTAPRILI
DQSGFSGITFSFIFASVALVMIVTARFAKGFVTRWGIAGSLARGMAMLLLGAVMLAAGEMFLQAPLIGFVLPMWIIAIGI
VFATAVTANGALKAFDDTAGTAVALYFCIESLLVGVAGTLFVVLLDGDTAWPLAAYAAVTALITLAGLRRLSCKTA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
floR YP_005176183.1 florfenicol / chloramphenicol efflux protein Not tested ICEPmu1 Protein 3e-93 64
flo AAK02049.1 chloramphenicol and florfenicol resistance protein Not tested SGI1 Protein 2e-95 63
floRc AGK06972.1 chloramphenicol and florfenicol resistance protein Not tested SGI1 Protein 2e-95 63
floRc AGK07102.1 chloramphenicol and florfenicol resistance protein Not tested SGI1 Protein 2e-95 63
cmlA9 ABZ01840.1 CmlA9 chloramphenicol efflux protein Not tested SGI2 Protein 1e-94 62
cmlA CAJ77032.1 chloramphenicol and florfenicol resistance protein Not tested AbaR1 Protein 1e-94 62
cml(A)5 ACY75522.1 CmLA(5) Not tested Tn6060 Protein 3e-72 50
cmlA5 CAJ77046.1 Chloramphenicol resistance protein Not tested AbaR1 Protein 5e-72 50

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Rleg2_1232 YP_002280752.1 Bcr/CflA subfamily drug resistance transporter AF294653.1.gene4.p01 Protein 4e-72 50
Rleg2_1232 YP_002280752.1 Bcr/CflA subfamily drug resistance transporter DQ984668.gene.p01 Protein 2e-72 50
Rleg2_1232 YP_002280752.1 Bcr/CflA subfamily drug resistance transporter DQ522237.1.gene4.p01 Protein 2e-72 50
Rleg2_1232 YP_002280752.1 Bcr/CflA subfamily drug resistance transporter NC_010410.6003586.p0 Protein 2e-72 50
Rleg2_1232 YP_002280752.1 Bcr/CflA subfamily drug resistance transporter AF156486.gene.p01 Protein 4e-70 49