Gene Information

Name : entE (KPK_3966)
Accession : YP_002239770.1
Strain : Klebsiella pneumoniae 342
Genome accession: NC_011283
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 4010749 - 4012356 bp
Length : 1608 bp
Strand : -
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence : 
ATGATTGCATTTACCCGTTGGCCGGATGCGTTCGCGGCCCGCTATCGTCAAAAAGGCTACTGGCAGGATCTGCCGTTAAC
CAATCTGATCACCCGTCACGCGGAAAACGACGCCGTGGCGATTATCGATGGCGAACGGCAGATTAGCTACCGCCAGTTTA
ATCAGCTGGTGGACAACCTTGCCTGCTCGCTGCAGCGCCAGGGACTGAAGCGCGGGGAAACCGCGCTGGTGCAGTTGGGC
AACGTCGCCGAGTTTTACATCACCTTCTTTGCGCTGCTGCGCATCGGCGTCGCGCCAGTGAACGCGCTGTTCAGCCATCA
GCGCAGCGAGCTTAACGCCTACGCCGCGCAGATTAAACCGGCGCTGCTGATTGCCGATCGCGAGCACGCGCTGTTTGCTG
ACGATAGCTTCCTCCATGCGTTTATCGCTGAGCATCCTTCCCTGCGCGTCGCACTGTTGCGTAACGACGGCGGCGAGCGC
GATCTGGCGACGGAGATTAACCGCCCGGCGGATAACTTTGTCGCCAATCCAACCCCTGCCGACGAAGTGGCCTTTTTCCA
GCTCTCCGGCGGCAGCACCGGGACGCCGAAGCTGATCCCGCGCACCCATAACGATTACGACTACAGCATCCGCCGCAGCA
ACGAGATCTGCGGGATCAACGCTGAGACGCGCTACCTCAACGCGCTGCCGGCGGCGCATAACTATGCGATGAGTTCCCCG
GGTTCGCTCGGCGTCTTTCTCGCCGGCGGGCGGGTGATCCTCGCCGCCGACCCGAGCGCCACCCTCTGTTTCCCGCTGAT
TGAACAACATCAGATCAACGTTGCCTCGCTGGTGCCGCCGGCGGTCAGCCTGTGGCTGCAGGCGATCCATGAATGGGGCA
GCAATGCGCAACTGCAATCGCTGGAGCTGCTGCAGGTCGGCGGCGCGCGCCTGTCGGCGACCCTCGCCGCGCGCATTCCG
GCGGAAATCGGCTGCCAGCTGCAGCAGGTGTTCGGCATGGCGGAGGGGCTGGTGAATTACACCCGCCTTAACGACAGCCC
GGAGCGGATCATCCATACCCAGGGCTGCCCGATGTGCCCGGACGATGAAGTGTGGGTAGCGGATGCCGATGGCAACCCGC
TGCCGCGCGGCGAAGTGGGCCGCCTGATGACCCGCGGGCCGTATACCTTCCGCGGCTACTACAACAGCCCGCAGCATAAC
GCGGAAGCCTTTGACGCCGAGGGGTTTTACTGCTCCGGCGATCTGATTTCGATCGACGAAGACGGGTATATCACCGTCCA
GGGCCGGGAAAAAGATCAGATCAACCGCGGCGGCGAGAAGATCGCCGCTGAAGAGATCGAAAACCTGCTGCTGCGCCATG
AGGCGGTGATCCACGCCGCGCTGGTCAGCATCGAAGACAACCTGCTGGGCGAGAAAAGCTGCGCTTATCTGGTGGTGACC
TCGCCGCTGCGCGCCGTCGCGGTGCGCCGCTTCCTGCGCGAGCAGGGCGTGGCGGAATTTAAATTGCCGGATCGCGTGGA
GTGCGTTGCCGCGCTGCCGCTGACGCCGGTGGGCAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCCGAAGGCAAGC
TGGGCTGA

Protein sequence : 
MIAFTRWPDAFAARYRQKGYWQDLPLTNLITRHAENDAVAIIDGERQISYRQFNQLVDNLACSLQRQGLKRGETALVQLG
NVAEFYITFFALLRIGVAPVNALFSHQRSELNAYAAQIKPALLIADREHALFADDSFLHAFIAEHPSLRVALLRNDGGER
DLATEINRPADNFVANPTPADEVAFFQLSGGSTGTPKLIPRTHNDYDYSIRRSNEICGINAETRYLNALPAAHNYAMSSP
GSLGVFLAGGRVILAADPSATLCFPLIEQHQINVASLVPPAVSLWLQAIHEWGSNAQLQSLELLQVGGARLSATLAARIP
AEIGCQLQQVFGMAEGLVNYTRLNDSPERIIHTQGCPMCPDDEVWVADADGNPLPRGEVGRLMTRGPYTFRGYYNSPQHN
AEAFDAEGFYCSGDLISIDEDGYITVQGREKDQINRGGEKIAAEEIENLLLRHEAVIHAALVSIEDNLLGEKSCAYLVVT
SPLRAVAVRRFLREQGVAEFKLPDRVECVAALPLTPVGKVDKKQLRQWLAEGKLG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-90 42
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-90 42
ybtE CAA21394.1 - Virulence HPI Protein 6e-90 42
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 6e-90 42
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 4e-90 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-90 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 7e-90 42
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-90 42
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 6e-90 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_002239770.1 enterobactin synthase subunit E VFG0932 Protein 0.0 81
entE YP_002239770.1 enterobactin synthase subunit E VFG0168 Protein 2e-109 45