Gene Information

Name : Lferr_0161 (Lferr_0161)
Accession : YP_002218629.1
Strain : Acidithiobacillus ferrooxidans ATCC 53993
Genome accession: NC_011206
Putative virulence/resistance : Resistance
Product : FAD-dependent pyridine nucleotide-disulfide oxidoreductase
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1249
EC number : -
Position : 149933 - 151354 bp
Length : 1422 bp
Strand : +
Note : PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; FAD dependent oxidoreductase; Heavy metal transport/detoxification protein; KEGG: ajs:Ajs_1298 mercuric reductase

DNA sequence :
ATGTCTCAGAAAATCTTTTTACGCATCACCGGGATGACCTGTGCGCATTGCGCCCACAGTGTAGAAAAGGCGCTGCTCGG
TATCCATGGTATCGACAGCGCCCAGGTTTCTTTGGCCACGAATCAGGCAGAAGTTTTCCTGCAGTCTTCCATACCGACAG
AAGCATTGCTGGCGGCCGTAACGCAGGCGGGCTATGGTGCCAAAGTAGAGCAGGATTCTTTGCAAGTTCAGGCTCGATCT
ACGCAGGAACCAGGACAGCCACACATCGCCATCATTGGCAGCGGCGGAGCGGCGATGGCTGCGGCGCTTAAAGCCGTTGA
ACGCGGCGCGCGGGTGACCTTGATCGAACGGGGTACCATCGGTGGCACCTGCGTCAAAGTCGGTTGCGTACCCTCCAAAA
TACTGATCCGGGCCGCGCACATCGCCCATCTGCGGCGCGCAAGTCCCTTTGATGCCGGGATTGCCGCGCAAGAGCCGGTT
ATTCGCCGCGACAGATTGCTGGCCCAGCAGCAAGGCCGCGTAGATGAGCTACGCTATGCCAAGTACGAAGGCATCCTGGC
GCAAACTCCGGCCATCAGCGTGCTGCGCGGCGAAGCCACGTTCCAGAACGCGCAGACGTTGTCCGTGGTGCTCGCCGACG
GCGGTGTTCACGAACTGCGGTTTGACCGCTGCCTGATTGCCACCGGGGCGAGCCCGGCAATTCCACCGCTCCCCGGTCTT
GCGGATACCCCCTACTGGACCTCCACCGAGGCGCTGGCGAGTGAAACCATCCCAGAACGGTTGGTCGTGATCGGTGCGTC
AGTAGTGGCGGTAGAACTCGCCCAGGCATTCGCCCGGCTCGGTAGCAAGGTGACGATCCTGGCGCGCAGCGTCCTGCTCA
GTCATGAGGATCCTGCCATTGGTGAGGCGATTACCAGCGCGTTCCGCGCCGAAGGGATTCATGTGCTGGAACATACCCAG
CCTAACCGGGCCGACTACAACGATGGTCAATTCCTGCTCACGACGGGAAACGGCCATCTGCGCGCCGATCGGCTATTGAT
CGCCACTGGCCGTGCGCCGAATACGCATAGCCTGAACCTTGCGGCGGTGGGCGTTGAGGTCAACGCACACGGAGCCATCG
TCATCGACAAGACGATGCGCACCAGCGCACCGTATATCCGCGATCTCAAGCGTCGTCTCGATGGGGGTCAAAGGAAATTG
CTCGCGAATGTCCGTAACACAACCGGCTCGTATATCATCCAGCATGAGGAAGTAGCTCAACTCCAACTGGGAGAGCAAGT
GGTGCACACGGCCCGTTTTTCGGCCTTTTATACGGACAGACAAGCCCCGAGAAGGTACGTCGCGCACGGTGAGCCACGGT
TTATAGCTTTTCTCACTGCCTTGGCCACGTCCTTCTTTCAGACGGCGCGCAATGGCCCCTGA

Protein sequence :
MSQKIFLRITGMTCAHCAHSVEKALLGIHGIDSAQVSLATNQAEVFLQSSIPTEALLAAVTQAGYGAKVEQDSLQVQARS
TQEPGQPHIAIIGSGGAAMAAALKAVERGARVTLIERGTIGGTCVKVGCVPSKILIRAAHIAHLRRASPFDAGIAAQEPV
IRRDRLLAQQQGRVDELRYAKYEGILAQTPAISVLRGEATFQNAQTLSVVLADGGVHELRFDRCLIATGASPAIPPLPGL
ADTPYWTSTEALASETIPERLVVIGASVVAVELAQAFARLGSKVTILARSVLLSHEDPAIGEAITSAFRAEGIHVLEHTQ
PNRADYNDGQFLLTTGNGHLRADRLLIATGRAPNTHSLNLAAVGVEVNAHGAIVIDKTMRTSAPYIRDLKRRLDGGQRKL
LANVRNTTGSYIIQHEEVAQLQLGEQVVHTARFSAFYTDRQAPRRYVAHGEPRFIAFLTALATSFFQTARNGP

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
merA AAN62178.1 mercuric (Hg(II)) reductase Not tested PAGI-2(C) Protein 5e-104 65
merA ABQ57371.1 MerA Not tested SGI1 Protein 2e-98 64
merA AGK07021.1 MerA Not tested SGI1 Protein 2e-98 64
merA AGK07079.1 MerA Not tested SGI1 Protein 2e-98 64
merA YP_006098387.1 mercuric ion reductase Not tested Tn2411 Protein 1e-93 63
merA AET25397.1 MerA Not tested PAGI-2(C) Protein 9e-94 63
merA AFG30120.1 MerA Not tested PAGI-2 Protein 9e-94 63
merA ACN81005.1 MerA Hg(II) mercuric ion reductase Not tested AbaR5 Protein 1e-96 61

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Lferr_0161 YP_002218629.1 FAD-dependent pyridine nucleotide-disulfide oxidoreductase BAC0653 Protein 3e-98 63
Lferr_0161 YP_002218629.1 FAD-dependent pyridine nucleotide-disulfide oxidoreductase BAC0652 Protein 1e-95 62
Lferr_0161 YP_002218629.1 FAD-dependent pyridine nucleotide-disulfide oxidoreductase BAC0648 Protein 2e-75 59
Lferr_0161 YP_002218629.1 FAD-dependent pyridine nucleotide-disulfide oxidoreductase BAC0650 Protein 3e-89 59