Gene Information

Name : lysS (SeD_A3376)
Accession : YP_002217018.1
Strain : Salmonella enterica CT_02021853
Genome accession: NC_011205
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 3246307 - 3247824 bp
Length : 1518 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCTGAACAAAACGCACAGGGCGCTGACGAGGTAGTCGATCTTAACAATGAAATGAAGGCACGTCGCGAGAAGCTGGC
TGCACTGCGTGAGCAGGGTATCCCGTTCCCGAACGATTTCCGTCGTGACCGTACCTCAGACCAACTGCACGCAGAGTTTG
ACGCGAAAGAAGCTGAAGAACTGGAAGCGCTGAATATTGAAGTGTCCGTCGCGGGCCGTATGATGACTCGCCGTATTATG
GGTAAAGCCTCTTTTGTTACCCTGCAAGACGTAGGCGGCCGCATTCAGCTGTATGTGGCGCGCGATGATCTGCCGGAAGG
CGTTTACAACGAGCAGTTCAAGAAGTGGGATCTTGGCGATATCCTCGGCGCGAAAGGTAAGCTGTTCAAAACCAAAACCG
GCGAGCTGTCTATCCACTGCACCGAACTGCGCCTGCTGACGAAAGCGCTGCGTCCGTTGCCGGATAAATTCCACGGCCTG
CAGGACCAGGAAGCGCGCTATCGCCAGCGTTATCTGGATCTCATCTCTAACGATGAATCCCGTAACACCTTCAAAACCCG
TTCGAAGATCCTGGCTGGTATTCGCCAGTTTATGGTTGCTCGCGGCTTTATGGAAGTCGAAACGCCGATGATGCAGGTGA
TCCCAGGCGGCGCGTCTGCCCGTCCGTTTATCACCCATCACAACGCGCTGGATCTGGACATGTACCTGCGTATCGCGCCG
GAACTGTACCTCAAGCGTCTGGTGGTTGGCGGCTTCGAACGCGTATTCGAAATTAACCGTAACTTCCGTAACGAAGGCAT
CTCCGTGCGCCATAATCCTGAGTTCACCATGATGGAACTCTATATGGCGTATGCGGACTACAAAGACCTGATCGAGCTGA
CTGAGTCTCTGTTCCGTACGCTGGCGCAGGATGTTCTGGGCACCACCCAGGTTCCGTACGGTGATGAAGTGTTTGACTTC
GGTAAGCCGTTCGAAAAACTGACCATGCGTGAAGCGATCAAGAAATATCGTCCGGAAACAGATATGGCTGATCTGGACAA
CTTCGATTCCGCGAAGGCGATTGCTGAAAGCATCGGCATTCACGTCGAGAAGAGCTGGGGTCTGGGCCGTATCGTCACCG
AGATCTTTGACGAAGTCGCTGAAGCGCATCTGATTCAGCCGACCTTCATCACCGAATATCCGGCTGAAGTGTCTCCACTG
GCGCGTCGTAATGATGTGAACCCGGAAATCACTGACCGCTTCGAGTTCTTTATCGGCGGTCGTGAAATCGGCAATGGCTT
TAGCGAGTTGAACGATGCGGAAGACCAGGCGCAGCGTTTCCTGGATCAGGTTAATGCAAAAGCGGCGGGCGACGACGAAG
CCATGTTCTACGACGAAGACTACGTGACGGCGCTGGAACACGGGTTACCGCCGACAGCGGGTCTGGGAATTGGTATCGAC
CGTATGGTGATGCTGTTTACTAACAGCCACACGATTCGCGACGTTATTCTGTTCCCGGCGATGCGTCCGGTGAAATAA

Protein sequence :
MSEQNAQGADEVVDLNNEMKARREKLAALREQGIPFPNDFRRDRTSDQLHAEFDAKEAEELEALNIEVSVAGRMMTRRIM
GKASFVTLQDVGGRIQLYVARDDLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
QDQEARYRQRYLDLISNDESRNTFKTRSKILAGIRQFMVARGFMEVETPMMQVIPGGASARPFITHHNALDLDMYLRIAP
ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYKDLIELTESLFRTLAQDVLGTTQVPYGDEVFDF
GKPFEKLTMREAIKKYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFDEVAEAHLIQPTFITEYPAEVSPL
ARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVNAKAAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID
RMVMLFTNSHTIRDVILFPAMRPVK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 3e-170 69
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 4e-92 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_002217018.1 lysyl-tRNA synthetase VFG1668 Protein 2e-170 69