Gene Information

Name : entE (SSPA1988)
Accession : YP_002142827.1
Strain : Salmonella enterica AKU12601
Genome accession: NC_011147
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 2218626 - 2220239 bp
Length : 1614 bp
Strand : -
Note : 'bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence :
ATGATGCGTATACCTTTCACCCGTTGGCCGGATGAATTTGCCCGCCGTTATCGTGAAAAAGGCTACTGGCAAGACGTGCC
GTTGACCGATATTCTGACCCGCCACGCTGACAGCGATAAGATGGCGGTCATTGAAGGCGAGCGCGCATTCAGCTATCGCC
AGCTAAACCAGGCCGCGGATAATCTGGCCTGCAGTTTACGCCGTCAGGGCATCAAACCTGGCGAAACCGCTCTGGTGCAA
CTGGGGAATGTACCGGAACTGTATATCACCTTTTTCGCCCTGTTGAAGCTTGGCGTAGCGCCCGTTCTGGCGCTGTTTAG
CCATCAACGTACCGAACTTAACGCCTATGCGATGCAGATCGCGCCGACGCTGGTGATTGCCGATCGCCAACATACGCTGT
TCGCCGGGGAGGACTTTCTCAACACGTTTGTGGCTGAACACCGCTCTGTGCGGGTGGTGTTATTGCGCAACGACGACGGC
GATCACAGCCTGGACGCGGCGATGCGGCAGGCGGCGGAAGATTTCACCGCCACGCCATCACCTGCTGACGAAGTGGCCTA
CTTTCAGCTTTCCGGCGGTACTACCGGCACGCCAAAGCTTATTCCCCGTACCCATAACGACTATTACTACAGCGTGCGCC
GCAGCAATGAGATTTGCGGTTTCAACGAGGAGACGCGTTTTCTGTGCGCGATTCCCGCCGCGCATAACTACGCCATGAGT
TCGCCGGGCGCGCTGGGCGTTTTTCTCGCCAAAGGAACGGTAGTGCTGGCGACCGATCCTAGCGCCACGCTCTGTTTCCC
GCTGATCGAAAAACACCAGATTAATGCCACGGCGTTGGTGCCGCCCGCGGTCAGTCTATGGCTACAGGCTATCCAGGAGT
GGGGCGGCAATGCGCCGCTGGCGTCATTAAGGTTATTGCAGGTTGGCGGCGCGCGGCTTTCTGCGACGCTTGCCGCCCGT
ATTCCGGCTGAAATTGGCTGTCAGTTGCAGCAGGTCTTCGGCATGGCGGAAGGGTTAGTGAACTATACCCGGCTGGACGA
TAGCCCGGAACGGATTATCAATACCCAGGGAAGACCCATGTGTCCGGACGACGAAGTGTGGGTGGCGGATGCCGACGGGA
ATCCACTGCCGCCGGGCGAGATTGGTCGTCTGATGACGCGCGGCCCCTATACTTTTCGCGGCTATTTCAACAGTCCGCAA
CACAATGCCAGCGCCTTTGACGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCCATTGATCAAGACGGCTATATCAC
CGTTCACGGGCGTGAAAAAGATCAGATCAATCGGGGCGGCGAGAAGATAGCCGCCGAAGAGATAGAAAACCTGTTACTGC
GCCACCCGGCGGTGATCCATGCGGCGCTGGTCAGCATGGAAGATGAACTGCTGGGGGAGAAAAGTTGCGCATATCTGGTG
GTAAAAGAGCCGCTGCGAGCGGTACAGGTACGCCGTTTCCTGCGAGAGCAGGGCGTGGCGGAATTTAAATTACCGGATCG
CGTGGAGTGCGTTGCGTCACTGCCACTGACGCCGGTTGGTAAAGTCGATAAAAAACAATTACGCCAGCGGTTGGCGTCAC
GTTCACCGCTCTGA

Protein sequence :
MMRIPFTRWPDEFARRYREKGYWQDVPLTDILTRHADSDKMAVIEGERAFSYRQLNQAADNLACSLRRQGIKPGETALVQ
LGNVPELYITFFALLKLGVAPVLALFSHQRTELNAYAMQIAPTLVIADRQHTLFAGEDFLNTFVAEHRSVRVVLLRNDDG
DHSLDAAMRQAAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSNEICGFNEETRFLCAIPAAHNYAMS
SPGALGVFLAKGTVVLATDPSATLCFPLIEKHQINATALVPPAVSLWLQAIQEWGGNAPLASLRLLQVGGARLSATLAAR
IPAEIGCQLQQVFGMAEGLVNYTRLDDSPERIINTQGRPMCPDDEVWVADADGNPLPPGEIGRLMTRGPYTFRGYFNSPQ
HNASAFDANGFYCSGDLISIDQDGYITVHGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELLGEKSCAYLV
VKEPLRAVQVRRFLREQGVAEFKLPDRVECVASLPLTPVGKVDKKQLRQRLASRSPL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-89 42
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 2e-89 42
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-89 42
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-89 42
ybtE CAA21394.1 - Virulence HPI Protein 2e-89 42
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-89 42
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 1e-89 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-89 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 2e-89 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_002142827.1 enterobactin synthase subunit E VFG0932 Protein 0.0 87
entE YP_002142827.1 enterobactin synthase subunit E VFG0168 Protein 4e-116 46
entE YP_002142827.1 enterobactin synthase subunit E VFG1817 Protein 2e-90 42