Gene Information

Name : lysS (MADE_1006470)
Accession : YP_004426434.1
Strain : Alteromonas macleodii Deep ecotype
Genome accession: NC_011138
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 1311453 - 1313021 bp
Length : 1569 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGACTGACCAACAAACAGACGATAACAAATTGATTGCAGAGCGCCGTGCCAAATTGAGCGCTATTCGCGAGCAGTGCCG
TGCCAACGGTTTCCCAAACAGCTTCCGTCGTGAAAACTATGCGGATGAGCTGCAAGCTAAGTTCGGTGAATTCGACAAAG
AAACCTTGCAAGAGCAGGGCAACAAGGTAAGCATTGCTGGCCGTATTATGGCAAAGCGTGGTCCTTTCATGTTGCTTCAA
GACATGACAGGCCGTATTCAAGCTTACGCAGACAAAGACACGCAAAAAGCACTTAAAGCAAAATACGGTGCGCTAGATAT
TGGTGACATCATTGGCGTAGCGGGCGACCTGCACAAGTCAGGTAAAGGCGACCTTTACGTAAACATGGAGCAATACGAGC
TGCTAACAAAAGCACTTCGTCCGTTGCCAGAAAAATTCCACGGTTTGAGCGACCAGGAAACCAAGTATCGCCAGCGCTAT
GTTGACTTGATCACAAGCGAACAAACCCGCAAAACCTTTATGATCCGCAGTAAAATTGTGAATGGTATTCGCAATTACCT
GACCGAGCGTGACTACCTAGAAGTAGAAACGCCAATGCTACAGGTTATTCCAGGTGGCGCGACGGCGAAGCCGTTTGTCA
CTCACCACAATGCGCTAGATATCGACATGTATCTGCGTATTGCGCCAGAGCTTTACCTTAAGCGTTTGGTTGTTGGTGGC
TTTGAGCGTGTGTTCGAAATTAACCGAAACTTCCGTAACGAAGGCTTATCAACCCGTCATAACCCTGAGTTTACTATGCT
TGAATTCTACCAAGCGTATGCAGACTACAACGACCTGATGAACCTTACCGAAGATATGCTGCGCACGTTAGCGGAAGACA
TTCTTGGTACCAGCATTATCAAGAACACAACGAAAGACGCTGAAGGTAACGTAACGTCTGAAGTAGAGTATGACTTTGGT
AAGCCGTTCGACCGTTTGAGCATGGGCGAGGCGATTCTTAAATACTGGCCTGAAGCTGATGAAGCCGCTATTCGCGACCC
AGAAGCGAACCTAGATTCGCTTAAAGCGATGGCAAAGCAGCTTCATATCAAAGAGCCTGAAGTTGACGGTATTTGGGGCG
CGGGTAAATACCTGTGCGAAATCTTCGAAGCAACAGCTGAAGAGAAGCTTGAACAACCTACGTTTATCACTGAATACCCT
TGGGAAGTATCGCCGCTAGCACGTCGTAATGACAACAACCCGTTCATTACCGACCGTTTCGAGTTCTTCGTAGGCGGACG
TGAATTAGCCAACGGCTTTAGCGAGCTTAACGACCCAGAAGACCAAGCTGAACGTTTTGCAAAGCAAGTTGAAGAAAAAG
ACGCGGGCGATGATGAAGCCATGCACTTTGACGATGACTATATTCGTGCACTTGAGTACGGCTTACCGCCAACAGCGGGT
GAGGGTATTGGTATCGACCGCCTAGCTATGCTATTTACCGATAGCTCAACTATCAAAGACGTTATCTTGTTCCCACATAT
GAAGCCTCAAGCGCCGAAAGACGCGCAAGAAGCCGGTGAGCAAGCGTAA

Protein sequence :
MTDQQTDDNKLIAERRAKLSAIREQCRANGFPNSFRRENYADELQAKFGEFDKETLQEQGNKVSIAGRIMAKRGPFMLLQ
DMTGRIQAYADKDTQKALKAKYGALDIGDIIGVAGDLHKSGKGDLYVNMEQYELLTKALRPLPEKFHGLSDQETKYRQRY
VDLITSEQTRKTFMIRSKIVNGIRNYLTERDYLEVETPMLQVIPGGATAKPFVTHHNALDIDMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSTRHNPEFTMLEFYQAYADYNDLMNLTEDMLRTLAEDILGTSIIKNTTKDAEGNVTSEVEYDFG
KPFDRLSMGEAILKYWPEADEAAIRDPEANLDSLKAMAKQLHIKEPEVDGIWGAGKYLCEIFEATAEEKLEQPTFITEYP
WEVSPLARRNDNNPFITDRFEFFVGGRELANGFSELNDPEDQAERFAKQVEEKDAGDDEAMHFDDDYIRALEYGLPPTAG
EGIGIDRLAMLFTDSSTIKDVILFPHMKPQAPKDAQEAGEQA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 4e-131 54

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_004426434.1 lysyl-tRNA synthetase VFG1668 Protein 2e-131 54