Gene Information

Name : ddl (Ppha_1631)
Accession : YP_002018485.1
Strain : Pelodictyon phaeoclathratiforme BU-1
Genome accession: NC_011060
Putative virulence/resistance : Resistance
Product : D-alanyl-alanine synthetase A
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1181
EC number : 6.3.2.4
Position : 1718473 - 1719555 bp
Length : 1083 bp
Strand : +
Note : D-alanine--D-alanine ligase; DdlA; DdlB; cytoplasmic; catalyzes the formation of D-alanyl-D-alanine from two D-alanines in peptidoglycan synthesis; there are two forms of this enzyme in Escherichia coli

DNA sequence :
ATGCCACGTATTACAGTTGCTCTCATTTTTGGAGGCAGGTCAACCGAACACGAAATATCAATCATCTCTGCAAAATCAAT
AGCAGCCAATATCAGCGCCGAACGTTACAAGGTTATTCCGCTCTACATTACACATCAGGGAACCTGGCTCTGTGAGGGCA
TTGCCCGCGACATTCTGAACCTTGACCTCTCCGCTCTTTTGAGAAACTCATCACCTGAAGCAGCAGCGATGGCGCTTGAC
AGGATGGTTGAAGAGGCGGAGCAGAAACCCTTCAACCTCGATTTCAGGGCTATCGGCATTGAAGTGGCATTTCTGACGCT
TCATGGCACCTATGGAGAAGATGGCCGCATCCAGGGCTTTCTGGATACCTGCAACATTCCCTATACAGGCTGCGGTGTAC
TGGCATCAGCGCTCACTATGGATAAAGCGCTCACCAAACTCTGTGTTGCAGATGCAGGCATTGCTGTTGCTCCATCCATA
ACGCTTCTCAGTGCCGAGTACCATGCCGATACGGAAAAAGTTCACTCCCGGATCGAAGAGAAGTTTATCTATCCATTTTT
TGTCAAACCGGCAAACCTCGGCTCCTCAATTGGAATCTCAAAGGTGCATCACCGGGAACAACTCCCTGCAGCCCTAAAAA
GCGCCTGTTCACTCGACTCAAAAATAGTGGTTGAAAAAGCAATCACAGGGCGTGAAATTGAGGTTGCTGTACTTGGCAAT
GATGAACCGGAAGTCTCCGTTTGCGGCGAAATCGAACCCGGCAGCGATTTCTATGACTATCATGATAAATACATTCACAA
CAGTGCAAAACTGTTTATTCCGGCACGCATTCCCGACGAAATGCAGAGCGAGGTCAGGAGCATTGCACTCAAGGCATACA
AGGCGCTGGGATGCAGGGGTATGTCGAGAATAGACTTTTTTGTTGACGAAAAGAGGGGAACCATCGTCCTCAATGAAGTC
AATACCATTCCCGGCTTCACCGATATCAGCATGTTTCCTCGACTCATGGCTGCAAGCGGACACTCTTTTCCTGAACTTGC
AGAGCGATTGCTGCAGCTTGCACTGGAAACCCTGCGACCATGA

Protein sequence :
MPRITVALIFGGRSTEHEISIISAKSIAANISAERYKVIPLYITHQGTWLCEGIARDILNLDLSALLRNSSPEAAAMALD
RMVEEAEQKPFNLDFRAIGIEVAFLTLHGTYGEDGRIQGFLDTCNIPYTGCGVLASALTMDKALTKLCVADAGIAVAPSI
TLLSAEYHADTEKVHSRIEEKFIYPFFVKPANLGSSIGISKVHHREQLPAALKSACSLDSKIVVEKAITGREIEVAVLGN
DEPEVSVCGEIEPGSDFYDYHDKYIHNSAKLFIPARIPDEMQSEVRSIALKAYKALGCRGMSRIDFFVDEKRGTIVLNEV
NTIPGFTDISMFPRLMAASGHSFPELAERLLQLALETLRP

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB YP_008394259.1 Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase Not tested Not named Protein 1e-41 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
ddl YP_002018485.1 D-alanyl-alanine synthetase A AF310956.2.orf5.gene Protein 1e-41 41
ddl YP_002018485.1 D-alanyl-alanine synthetase A AY145441.gene.p01 Protein 1e-41 41
ddl YP_002018485.1 D-alanyl-alanine synthetase A AF162694.gene.p01 Protein 7e-42 41