Gene Information

Name : fliI (Mpop_0656)
Accession : YP_001923369.1
Strain : Methylobacterium populi BJ001
Genome accession: NC_010725
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 701903 - 703231 bp
Length : 1329 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGACGGACAACGCCATCGAGCGCCTCCGCAAGGCGATGGAAGCCGGGCGCCGCGATCGGCCGCTGGTGCGGGTCGGCGG
ACCCGTGCGCGAGGTCACCGCGAGCGCCGCGCGGGTCGCGGGCGTCTCCGCCTTCGTGCGGCTCGGCGACCGCATGGGCT
TCTCGGCCGATGGCCGCGTGCAGGTCGGCGAGGTCGTGCGCATCGATTCTGCCGGTGCCACGGTCAAGCCGTTCGAGAGC
CGGATCGAGGCCGGCATCGGCAGCCTCGCCCACCGCATCGGCCCGGCGCAGCTGCGCCCGGACCCGCGCTGGAAGGGCCG
GGTGATCGACGCGCTCGGCCGCCCCGTCGATGGCCTCGGCCCGCTGGCCGAGGGGCCGGACAGCGTCCCCCTCGACGCCG
ACCCGCCCGCCGCCATGACGCGTGCCCGGGTGCGGACGCCGCTGCCCACGGGGGTCCGGGCGGTCGACCTGTTCACGCCG
CTCTGCGCCGGCCAGCGCATCGGCATCTTCGCCGGCTCCGGCGTCGGCAAGTCCACCTTGCTGGCGATGCTGGCGGGCGC
CGAAGGCTTCGACAGCGTCGTGGTGGCGCTGGTGGGCGAGCGCGGACGCGAGGTGCGCGAGTTCCTCGAAGGACCGATTG
CCGCCAGCCGCGAGCGTGCGGTGATCGTGGTCTCGACCGGCGACGAGAGCCCGATGATGCGCCGTCAGGCGCCGAAGGTG
GCGCTTGCGGTGGCCGAGTGCTTCCGCGACCGCGGCGAGTCGGTGCTGCTCATCGTCGATTCCGTGACCCGCTACGCCCA
CGCCGCCCGCGACGTGGCGCTCGCGGCCGGCGAACCGGCGGTGGCCCGCGGCTATCCGCCGAGCGTCTTTTCGGACCTGC
CGCGCCTCCTGGAACGCGCCGGCCCCGGCGCCGAGGGGAGTGGCACCATCACCGGCATCTTCTCGGTGCTGGTCGATGGC
GACGACCACAACGACCCGGTCGCCGACTCGATCCGCGGCACCCTCGACGGCCACGTGGTGCTCGACCGCGCCATTGCCGA
ACAGGGCCGCTATCCAGCGATCGACCTGCTCGGCTCGATCTCGCGGCTCGCGGGCGAGGTCTGGACCGGCGACCAGCGCG
AATTGGTGCGCAAGCTCAAAAGCATGATGGCCCGTTTCGAGGAGACGCGGGACCTGCGCCTGATGGGCGGCTACCGCGCC
GGCAGCGACCCCGACCTCGATCAGGCCGTCAGCCTCGTGCCGCGGATCTACGAGGCCCTGCGCCAGGAGCCGGGTCAGCC
GCCGAGCCGCGACGCCTTCCTCGAACTTGCCCAGTCCCTCCGGGGATGA

Protein sequence :
MTDNAIERLRKAMEAGRRDRPLVRVGGPVREVTASAARVAGVSAFVRLGDRMGFSADGRVQVGEVVRIDSAGATVKPFES
RIEAGIGSLAHRIGPAQLRPDPRWKGRVIDALGRPVDGLGPLAEGPDSVPLDADPPAAMTRARVRTPLPTGVRAVDLFTP
LCAGQRIGIFAGSGVGKSTLLAMLAGAEGFDSVVVALVGERGREVREFLEGPIAASRERAVIVVSTGDESPMMRRQAPKV
ALAVAECFRDRGESVLLIVDSVTRYAHAARDVALAAGEPAVARGYPPSVFSDLPRLLERAGPGAEGSGTITGIFSVLVDG
DDHNDPVADSIRGTLDGHVVLDRAIAEQGRYPAIDLLGSISRLAGEVWTGDQRELVRKLKSMMARFEETRDLRLMGGYRA
GSDPDLDQAVSLVPRIYEALRQEPGQPPSRDAFLELAQSLRG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-63 47
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-64 47
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 1e-64 47
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-64 47
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 4e-64 47
lscN AAO18044.1 LscN Virulence TTSS locus Protein 4e-62 47
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 3e-65 47
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-65 47
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 3e-65 47
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 3e-56 46
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 3e-56 46
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 2e-57 46
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-57 46
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-57 46
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-57 46
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-67 45
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-67 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-55 45
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 2e-67 45
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 3e-55 45
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 2e-57 45
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 4e-61 44
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 4e-61 44
escN AAC38383.1 EscN Virulence LEE Protein 4e-60 44
escN AAC31513.1 L0034 Virulence LEE Protein 2e-60 44
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 2e-60 44
escN AAK26715.1 EscN Virulence LEE Protein 9e-59 44
escN YP_003223475.1 translocator EscN Virulence LEE Protein 3e-59 44
escN AAL57542.1 EscN Virulence LEE Protein 9e-59 44
escN NP_290268.1 hypothetical protein Virulence LEE Protein 3e-60 44
ECs4568 NP_312595.1 EscN Virulence LEE Protein 3e-60 44
escN CAC81862.1 EscN protein Virulence LEE II Protein 4e-59 44
escN YP_003236088.1 translocator EscN Virulence LEE Protein 3e-60 44
escN CAI43874.1 EscN protein Virulence LEE Protein 2e-59 44
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-65 43
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 5e-59 43
unnamed AAL06369.1 EscN Virulence LEE Protein 8e-59 43
invC AAS66861.1 InvC Not tested SSR-2 Protein 2e-55 43
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 5e-51 43
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 4e-52 43
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 6e-66 42
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 8e-66 42
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 1e-65 42
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 3e-57 41
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-57 41
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 3e-54 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_001923369.1 flagellum-specific ATP synthase VFG2331 Protein 2e-69 48
fliI YP_001923369.1 flagellum-specific ATP synthase VFG0515 Protein 2e-64 47
fliI YP_001923369.1 flagellum-specific ATP synthase VFG2144 Protein 1e-68 47
fliI YP_001923369.1 flagellum-specific ATP synthase VFG0555 Protein 9e-58 46
fliI YP_001923369.1 flagellum-specific ATP synthase VFG0048 Protein 3e-68 45
fliI YP_001923369.1 flagellum-specific ATP synthase VFG1254 Protein 2e-66 45
fliI YP_001923369.1 flagellum-specific ATP synthase VFG0812 Protein 1e-60 44
fliI YP_001923369.1 flagellum-specific ATP synthase VFG0730 Protein 2e-60 44
fliI YP_001923369.1 flagellum-specific ATP synthase VFG0192 Protein 3e-62 41
fliI YP_001923369.1 flagellum-specific ATP synthase VFG1008 Protein 5e-52 41