Gene Information

Name : nifJ (CLH_0756)
Accession : YP_001920151.1
Strain : Clostridium botulinum Alaska E43
Genome accession: NC_010723
Putative virulence/resistance : Virulence
Product : pyruvate:ferredoxin (flavodoxin) oxidoreductase
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG0674
EC number : 1.2.7.1
Position : 784313 - 787813 bp
Length : 3501 bp
Strand : +
Note : identified by match to protein family HMM PF00037; match to protein family HMM PF01558; match to protein family HMM PF01855; match to protein family HMM TIGR02176

DNA sequence :
ATGAGAAAAATGAAAACTATGGATGGTAATACTGCAGCAGCTCACGTATCTTATGCGTTTACAGAAGTAACTGCAATATA
TCCAATAACACCATCATCACCAATGGCAGAACATGTAGATGAATGGGTTGCACAAGGAAGAAAGAATATATTTGGACAAA
CTGTTAAAGTAATGGAGATGCAATCAGAAGCTGGAGCTGCTGGAGCAGTTCACGGTTCTTTACAAGCTGGAGCATTAACA
ACTACTTATACAGCTTCACAAGGTTTATTATTAATGATACCAAATATGTACAAGATATCAGGTGAAATGTTACCAGGAGT
ATTCCACGTTTCAGCTAGAGCTTTAGCTACATCTTCATTAAACATATTTGGAGATCATCAAGATGTTATGGCAGCAAGAC
AAACTGGATTTGCTATGCTTGCTGAAGGATCAGTTCAAGAAGTTATGGATTTATCAGCAGTAGCACATTTAACTGCTATA
AAATCTAGAATTCCATTCTTAAACTTCTTTGATGGTTTTAGAACTTCTCACGAAATTCAAAAAATTGAAGTATTAGACTA
TGCTGATTTAGCTAAGTTAGTTGATATGGACGCTGTTAAAGCTTTCAGAGCAAGAGCTTTAAATCCAGATCATCCTGTAA
CTAGAGGAACAGCTCAAAATGCTGATATCTATTTCCAAGAAAGAGAATCAGTAAATAAATTCTACAATGAATTACCAGAT
ATGGTTGAAAGCTATATGGCTGAAATCACTAAAATAACTGGTAGAGAATATCATTGTTTCGACTACTATGGTGCTGAAGA
TGCAGACAGAGTAGTTATAGCAATGGGTTCAGTAACAGATGTAGCTGAAGAAACTGTAGATTACTTAAATGCACATGGAC
AAAAAGTTGGTCTTGTTAAAGTAAGACTTTACAGACCATTCTCAGTTGAAAAATTAATAGCTGCTATTCCAAGCACTGTT
AAGAAAATCGCTGTTTTAGATAAGACTAAAGAACCAGGTGCTGACGGAGAACCATTATACTTAGATATTAGAAATGCATT
CTACGGAAAAGAAAATGCTCCTGTTATAATTGGCGGAAGATTTGGTTTAGGATCAAAAGATCCAAATCCAGCTCATATTG
CTGCTGTTTATGATAACTTAGCAAAAGCTGAACCAAAGAATGGATTCACTATAGGAATAGTTGATGACGTTACAAATACT
TCATTAGAAGTAACTGAAGATATAGATGCTACTCCAGAAGGAACTACAGCTTGTAAGTTCTGGGGATTAGGATCAGATGG
TACTGTTGGAGCAAACAAGAGTGCTATAAAGATCATTGGAGATCATACAGACATGTATGCTCAAGGATACTTCTTCTATG
ATTCAAAGAAATCAGGCGGAATTACAGTATCTCACTTAAGATTTGGTAAGAAGGCAATTAAGTCTCCATACTTAATCAAC
AAAGCAGATTTCGTATCTTGTTCTAATCAATCATACGTTCATAAATATAACGTATTAGAAGGATTAAAACCAGGAGCAAC
TTTCTTATTAAATACTATCTGGACTCCAGAAGAATTAGAAGAAAAGTTACCTGCTTCATATAAGAGATTTATGGCAAACA
ACAACATTAAGTTCTACACTTTAAATGCTGTTGCTATAGCTCAAGAAATAGGTCTTGGTGGAAGAATCAACATGATAATG
CAATCAGCTTTCTTCAAGCTAGCTAACATTATCCCAGTTGAAGACGCTGTTAAATACTTAAAAGACTCAGTTGTAACTTC
TTATGGTAAGAAGGGTGAAAAAGTTGTTAATATGAACAACGCAGCAATCGATAAGGGTGTTGAATCTATCGTTGAAATCA
AACTTCCAGAAGCTTGGAAGACTGCTAAAGACGAAGAAGTAGCTCCACTTAAGAATGCTTCAGAATTCGTTAAGAATATA
GTTGTTCCAATGAACAGACAAGAAGGAGATTCTCTTCCAGTTTCTGCTTTTGTTGGAATGGAAGATGGTACATTTGAAGC
TGGTACTGCAGCCTTCGAAAAGAGAGGAATCGCAGTTAACGTTCCTGAATGGGATGCTGAAAAATGTATTCAATGTAACC
AATGTTCATTAGTATGTCCACATGCTGCTATCAGACCAATATTATTAAATGAAGCAGAAAAGAATGCTGCACCTGCTAAT
GCAACAATAGTTGATGCTAAAGCTCTTAAGAGTGAAGAAAAATTATTCTACACTATGGCAGTAGCACCACTTGATTGTTC
AGGTTGTGGAAACTGTGCTCAAATTTGTCCTGCACCAGGAAAAGCATTAGTTATGAAACCACAAGAAAGCCAACACAATC
AAATTGAAGTTTGGGATTACTTAGTAGATGAAGTATCAACTAAAAAGAACCCAATGAATAAGAATACAGTAAAAGGTAGC
CAATTTGAGCAACCATTACTTGAATTCTCAGGAGCTTGTGCTGGTTGTGGAGAAACTCCATACGCTAAGCTTATAACTCA
ATTATTTGGAGATAGAATGATGATCGCTAATGCAACTGGATGTTCATCAATCTGGGGTGGATCTGCACCTTCAACTCCAT
ACACTAAGAATAAAGAAGGACATGGTCCAGCTTGGGCTAATTCATTATTCGAAGATAATGCTGAATACGGATTAGGTATG
TTCTTAGGAGTAAGAGCTATAAGAGAAAGAATCCAAGAAAGAGCAGAAGCTGCTATAGCTGCAAATGATCCAGCTAAAGC
TGAATTACAAGATTGGTTAGAAAATATGAACGAAGGTGCTGGAACTAGAGATAGAGCTGCTAAATTAGTTGCTGCTTTAG
AAAAATCTGGTACAGAAGCTGCTAAAGAAATTTTAGCTGAAAAAGACTACTTCGTTAAGAGATCTCAATGGATCTTCGGA
GGAGACGGATGGGCTTACGACATCGGATACGGTGGAGTTGACCACGTACTTGCTTCAGGAGAAGATGTAAATGTATTTGT
ATTTGATACAGAAGTTTACTCAAACACAGGTGGACAATCTTCTAAATCTACACCAACAGCTGCAATAGCTAAATTCGCTG
CTGCAGGTAAGAGAACTAAGAAGAAAGACCTTGGAATGATGGCTATGACTTACGGTTACGTATATGTAGCTCAAATCAAT
ATGGGAGCTGATAGAAATCAAGTTCTTAAAGCTATTGCAGAAGCAGAAGCTTACAAGGGACCATCATTAATCATAGCTTA
TGCTCCATGTATAAACCATGGAATTAAGCTTGGTATGAGCAACAGCCAATTAGAAGCTAAGAGAGCAACTGAATGTGGAT
ACTGGGCAATGTACAGATTTAACCCAGAATTAAAGGGAACTAAGAACCCATTCACATTAGATTCTAAAGCTCCAACTGCT
GACTTTAAAGAATTCTTAATGGGTGAAGTAAGATACGCTTCACTTGCTAAAGCATTCCCAGAAGCTGCAGAAGCATTATT
TGAAAAGACTTATACTGATGCTATGGAAAGATTAGAAGGATACAAGAAATTAGCTGAATAG

Protein sequence :
MRKMKTMDGNTAAAHVSYAFTEVTAIYPITPSSPMAEHVDEWVAQGRKNIFGQTVKVMEMQSEAGAAGAVHGSLQAGALT
TTYTASQGLLLMIPNMYKISGEMLPGVFHVSARALATSSLNIFGDHQDVMAARQTGFAMLAEGSVQEVMDLSAVAHLTAI
KSRIPFLNFFDGFRTSHEIQKIEVLDYADLAKLVDMDAVKAFRARALNPDHPVTRGTAQNADIYFQERESVNKFYNELPD
MVESYMAEITKITGREYHCFDYYGAEDADRVVIAMGSVTDVAEETVDYLNAHGQKVGLVKVRLYRPFSVEKLIAAIPSTV
KKIAVLDKTKEPGADGEPLYLDIRNAFYGKENAPVIIGGRFGLGSKDPNPAHIAAVYDNLAKAEPKNGFTIGIVDDVTNT
SLEVTEDIDATPEGTTACKFWGLGSDGTVGANKSAIKIIGDHTDMYAQGYFFYDSKKSGGITVSHLRFGKKAIKSPYLIN
KADFVSCSNQSYVHKYNVLEGLKPGATFLLNTIWTPEELEEKLPASYKRFMANNNIKFYTLNAVAIAQEIGLGGRINMIM
QSAFFKLANIIPVEDAVKYLKDSVVTSYGKKGEKVVNMNNAAIDKGVESIVEIKLPEAWKTAKDEEVAPLKNASEFVKNI
VVPMNRQEGDSLPVSAFVGMEDGTFEAGTAAFEKRGIAVNVPEWDAEKCIQCNQCSLVCPHAAIRPILLNEAEKNAAPAN
ATIVDAKALKSEEKLFYTMAVAPLDCSGCGNCAQICPAPGKALVMKPQESQHNQIEVWDYLVDEVSTKKNPMNKNTVKGS
QFEQPLLEFSGACAGCGETPYAKLITQLFGDRMMIANATGCSSIWGGSAPSTPYTKNKEGHGPAWANSLFEDNAEYGLGM
FLGVRAIRERIQERAEAAIAANDPAKAELQDWLENMNEGAGTRDRAAKLVAALEKSGTEAAKEILAEKDYFVKRSQWIFG
GDGWAYDIGYGGVDHVLASGEDVNVFVFDTEVYSNTGGQSSKSTPTAAIAKFAAAGKRTKKKDLGMMAMTYGYVYVAQIN
MGADRNQVLKAIAEAEAYKGPSLIIAYAPCINHGIKLGMSNSQLEAKRATECGYWAMYRFNPELKGTKNPFTLDSKAPTA
DFKEFLMGEVRYASLAKAFPEAAEALFEKTYTDAMERLEGYKKLAE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed AFJ97267.1 pyruvate ferredoxin Virulence Not named Protein 0.0 59