Gene Information

Name : pyk (CLH_0542)
Accession : YP_001919940.1
Strain : Clostridium botulinum Alaska E43
Genome accession: NC_010723
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 540809 - 542227 bp
Length : 1419 bp
Strand : -
Note : identified by match to protein family HMM PF00224; match to protein family HMM PF02887; match to protein family HMM TIGR01064

DNA sequence :
ATGCAAAAAACTAAAATGATTTTTACTATTGGACCTGCAAGTGATAATGAGGAGATTATAAGAAAGTTTATAGGCATTGG
AATGAATGCAGCAAGATTAAATTTTTCACATGGTACTTATGAATCACACAGAGAGAAAATAGAATTAATTAAAAGAGTAA
GAGAAGATATGGGAACTCCTACTGCAATTATCTTAGATATAAAAGGACCTAAGATAAGAACACATAATTTTGTAAACGAT
GGCGTAGAATTAAAAACTGGTCAAGAATTCTGTTTTGTATGCGGAGATGAAATCCTTGGTGATGAAGAAAGATGTTCTAT
TACTTATGAAGAACTTTACAAAGACATACAAATAGGTGGAGAAATCCTTGTTGATGATGGTCTTTTAAGATTCAAAGTCA
TAGCTGTTGAAGGTAAAGCTATTAGATGTAATGTAACTGTTGGTGGAATGATTAAAAATCACAAAGGTGTTAACGTTCCC
AACGTAATAATAAGACTACCTTCAGTAACAGAAAAAGATATAGCTGATATAAAGTTTGGTTGTGAAATGGGTGTAGACTT
TATTGCAGCATCTTTTATAAGAAAAGCTAGTGATGTAGCTGATGTAAAGAAAATACTTTCTGAAAACAATGGAAAACATA
TTAAAGTAATAGCTAAAATTGAAAATCAAGAAGGCGTAGATAACATAGATTCTATAATAGAACAAACTGATGCAGTAATG
GTAGCTAGAGGAGATATGGGAGTTGAAATTCCGATTCAAAAAGTTCCTATAATTCAAAAAATGATAATTAGAAAATGTAA
TGATGCTGGAAAGTTTGTTGTAACAGCAACTCAAATGCTTGATTCTATGATTAGAAATTCTTTACCAACTAGAGCAGAAG
CAAGTGATATTTGTAATGCTATATTTGATGGAACTGATGCTATCATGTTAAGTGGTGAAAGTGCATCTGGAAATTTCCCA
ATTGAAGCTGCAACAACAATGTCTGAAATCGCACAAGAAGCAGAAGCTCATTTGGATTATGCTCATTTAACAGCAAGATT
AAGAGAACCATCTCTTACTGATTATGCTGGTGCAATAAGTTATACAGCTTGTAGAACTTCAAATTTATTAAATGCATCTG
CAATAGTGGCTGCTACTAAGAGTGGTGCTACTGCTACTATGATTTCAAGATACAGACCAAAGTCACCTGTTATAGCAATA
ACACCTTACGAACAAGTAAGAAGAAGCTTAGCTTTAACTTTTGGTGTATTACCAATGCTTTGCGAAATGTACAATACTAC
TGATGAGATATTAGTAGAAGCTAGAAGATCAGTATTCGAATTAAACATTGCTCTACCAGGCGATGATATTGTAGTTGCTG
CTGGAATGCCAACAACTCAAACTGGTGGAACAAATATGTTAAAAATAGAGAAATTATAA

Protein sequence :
MQKTKMIFTIGPASDNEEIIRKFIGIGMNAARLNFSHGTYESHREKIELIKRVREDMGTPTAIILDIKGPKIRTHNFVND
GVELKTGQEFCFVCGDEILGDEERCSITYEELYKDIQIGGEILVDDGLLRFKVIAVEGKAIRCNVTVGGMIKNHKGVNVP
NVIIRLPSVTEKDIADIKFGCEMGVDFIAASFIRKASDVADVKKILSENNGKHIKVIAKIENQEGVDNIDSIIEQTDAVM
VARGDMGVEIPIQKVPIIQKMIIRKCNDAGKFVVTATQMLDSMIRNSLPTRAEASDICNAIFDGTDAIMLSGESASGNFP
IEAATTMSEIAQEAEAHLDYAHLTARLREPSLTDYAGAISYTACRTSNLLNASAIVAATKSGATATMISRYRPKSPVIAI
TPYEQVRRSLALTFGVLPMLCEMYNTTDEILVEARRSVFELNIALPGDDIVVAAGMPTTQTGGTNMLKIEKL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-90 44
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-89 44
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-90 44
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-90 44

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk YP_001919940.1 pyruvate kinase VFG0479 Protein 6e-91 44